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• W2002877066 abstract "Objective. The purpose of this study was to determine whether oligoclonally expanding tumor-infiltrating lymphocytes (TIL) were tumor-specific. Study design. Peripheral blood lymphocytes (PBL) from an ovarian tumor-bearing patient were stimulated in vitro with an autologous cancer cell line (SMOV-2). Then genes coding for the third complementarity-determining region of the T cell receptor (TCR) β chain were amplified by reverse transcription-polymerase chain reaction and separated by single-strand conformation polymorphism. Accumulated TCR clonotypes in vitro and in vivo in TIL were compared. Results. Clonal expansion of T cells was generated from PBL by stimulation with SMOV-2. A portion of the proliferated clonotypes was found to be identical to those accumulated in TIL in vivo. Conclusion. This is the first demonstration that accumulating T cell clones in TIL recognize antigen(s) on an autologous tumor. Further characterization of such T cell clonotypes may lead to tumor antigen-specific immunotherapy." @default.
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• W2002877066 date "1999-07-01" @default.
• W2002877066 modified "2023-09-26" @default.
• W2002877066 title "Clonal Expansion of T Cells That Are Specific for Autologous Ovarian Tumor among Tumor-Infiltrating T Cells in Humans1" @default.
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• W2002877066 doi "https://doi.org/10.1006/gyno.1999.5430" @default.
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