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• W2002902680 abstract "The inflexibility of double-stranded DNA with respect to bending and twisting is well established in vitro. Understanding apparent DNA physical properties in vivo is a greater challenge. Here, we exploit repression looping with components of the Escherichia coli lac operon to monitor DNA flexibility in living cells. We create a minimal system for testing the shortest possible DNA repression loops that contain an E. coli promoter, and compare the results to prior experiments. Our data reveal that loop-independent repression occurs for certain tight operator/promoter spacings. When only loop-dependent repression is considered, fits to a thermodynamic model show that DNA twisting limits looping in vivo, although the apparent DNA twist flexibility is 2- to 4-fold higher than in vitro. In contrast, length-dependent resistance to DNA bending is not observed in these experiments, even for the shortest loops constraining <0.4 persistence lengths of DNA. As observed previously for other looping configurations, loss of the nucleoid protein heat unstable (HU) markedly disables DNA looping in vivo. Length-independent DNA bending energy may reflect the activities of architectural proteins and the structure of the DNA topological domain. We suggest that the shortest loops are formed in apical loops rather than along the DNA plectonemic superhelix." @default.
• W2002902680 created "2016-06-24" @default.
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• W2002902680 creator A5089535217 @default.
• W2002902680 date "2010-12-01" @default.
• W2002902680 modified "2023-09-28" @default.
• W2002902680 title "Gene repression by minimal lac loops in vivo" @default.
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• W2002902680 doi "https://doi.org/10.1093/nar/gkq755" @default.
• W2002902680 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3001091" @default.
• W2002902680 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21149272" @default.
• W2002902680 hasPublicationYear "2010" @default.
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