FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003011323> ?p ?o ?g. }

• W2003011323 endingPage "335" @default.
• W2003011323 startingPage "327" @default.
• W2003011323 abstract "MONO-MAC-1 is a human cell line with properties of blood monocytes, which can be used as a model system to study monocytic functions in vitro. In the present study, we prepared a karyotype of MONO-MAC-1, analysed the growth behaviour, determined the presence of differentiation-associated antigens and studied the expression and secretion of several cytokines upon stimulation with 12-O-tetradecanoyl phorbol 13-acetate (TPA) and lipopolysaccharide (IPS). The MONO-MAC-1 cells have a near diploid karyotype and contain several recurrent chromosomal rearrangements, in particular the translocation (9;11) commonly found in AML-M5. Stimulation with TPA or LPS induced changes in morphology and gene expression, especially an increase in the level of the differentiation marker CD14 and the production of monocyte-related cytokines. Both biomodulators alone were sufficient to promote TNFα release; however, the combination of TPA and LPS resulted in a synergistic increase of TNFα secretion. Northern blot analysis indicated that upregulated production of TNFα was due to induced synthesis of mRNA. The mRNA accumulation peaked approximately 2 h after stimulation and maximum levels of TNFα were found in the supernatants after 4–8 h of culture. The MONO-MAC-1 cells could not be restimulated with the same inducer to release TNFα when a 48 h pre-treatment was carried out with LPS or TPA. LPS induced the release of granulocyte colony-stimulating factor (G-CSF), while TPA failed to do so. Vice versa, secretion of macrophage CSF (M-CSF) could be induced by TPA, but not by LPS. However, LPS enhanced the TPA-induced M-CSF production. Similarly, incubation of MONO-MAC-1, simultaneously with TPA and LPS, led to granulocyte macrophage CSF (GM-CSF) and interleukin-1β (IL-1β)secretion, while both stimulators alone had almost no (TPA) or only a weak (LPS) effect on the secretion of GM-CSF and IL-1β. Our results demonstrate that MONO-MAC-1 is a unique cell line with distinct monocytic features; certain monocytic properties can be upregulated by activation of intracellular signalling pathway(s). We suggest that, besides the LPS receptor CD14, activation of PKC participates in these process, especially in the production and secretion of cytokines by MONO-MAC-1 cells." @default.
• W2003011323 created "2016-06-24" @default.
• W2003011323 creator A5011231389 @default.
• W2003011323 creator A5018689307 @default.
• W2003011323 creator A5058240887 @default.
• W2003011323 creator A5072676390 @default.
• W2003011323 creator A5084799901 @default.
• W2003011323 date "1997-04-01" @default.
• W2003011323 modified "2023-09-23" @default.
• W2003011323 title "A model system in haematology and immunology: The human monocytic cell line MONO-MAC-1" @default.
• W2003011323 cites W1519301821 @default.
• W2003011323 cites W1539675729 @default.
• W2003011323 cites W1542029261 @default.
• W2003011323 cites W1556949853 @default.
• W2003011323 cites W1594758412 @default.
• W2003011323 cites W1602759787 @default.
• W2003011323 cites W1666992496 @default.
• W2003011323 cites W1776880984 @default.
• W2003011323 cites W1880660609 @default.
• W2003011323 cites W1932535232 @default.
• W2003011323 cites W1944945266 @default.
• W2003011323 cites W1972122119 @default.
• W2003011323 cites W1978993962 @default.
• W2003011323 cites W1981943315 @default.
• W2003011323 cites W2004116077 @default.
• W2003011323 cites W2013994536 @default.
• W2003011323 cites W2029157767 @default.
• W2003011323 cites W2039081691 @default.
• W2003011323 cites W2041233605 @default.
• W2003011323 cites W2053350170 @default.
• W2003011323 cites W2055090334 @default.
• W2003011323 cites W2056334753 @default.
• W2003011323 cites W2059929270 @default.
• W2003011323 cites W2062784484 @default.
• W2003011323 cites W2064626913 @default.
• W2003011323 cites W2067299054 @default.
• W2003011323 cites W2080831004 @default.
• W2003011323 cites W2101835477 @default.
• W2003011323 cites W2105415988 @default.
• W2003011323 cites W2110902399 @default.
• W2003011323 cites W2133764756 @default.
• W2003011323 cites W2141001643 @default.
• W2003011323 cites W2174322658 @default.
• W2003011323 cites W2421037982 @default.
• W2003011323 cites W4237822095 @default.
• W2003011323 cites W4248460106 @default.
• W2003011323 cites W95673823 @default.
• W2003011323 doi "https://doi.org/10.1016/s0145-2126(96)00129-4" @default.
• W2003011323 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9150350" @default.
• W2003011323 hasPublicationYear "1997" @default.
• W2003011323 type Work @default.
• W2003011323 sameAs 2003011323 @default.
• W2003011323 citedByCount "26" @default.
• W2003011323 countsByYear W20030113232014 @default.
• W2003011323 countsByYear W20030113232015 @default.
• W2003011323 countsByYear W20030113232016 @default.
• W2003011323 countsByYear W20030113232019 @default.
• W2003011323 countsByYear W20030113232020 @default.
• W2003011323 crossrefType "journal-article" @default.
• W2003011323 hasAuthorship W2003011323A5011231389 @default.
• W2003011323 hasAuthorship W2003011323A5018689307 @default.
• W2003011323 hasAuthorship W2003011323A5058240887 @default.
• W2003011323 hasAuthorship W2003011323A5072676390 @default.
• W2003011323 hasAuthorship W2003011323A5084799901 @default.
• W2003011323 hasConcept C104317684 @default.
• W2003011323 hasConcept C108634530 @default.
• W2003011323 hasConcept C109159458 @default.
• W2003011323 hasConcept C134018914 @default.
• W2003011323 hasConcept C150194340 @default.
• W2003011323 hasConcept C153911025 @default.
• W2003011323 hasConcept C177707886 @default.
• W2003011323 hasConcept C17991360 @default.
• W2003011323 hasConcept C185592680 @default.
• W2003011323 hasConcept C195794163 @default.
• W2003011323 hasConcept C203014093 @default.
• W2003011323 hasConcept C24998067 @default.
• W2003011323 hasConcept C2778513237 @default.
• W2003011323 hasConcept C2778754761 @default.
• W2003011323 hasConcept C2780043322 @default.
• W2003011323 hasConcept C2781184567 @default.
• W2003011323 hasConcept C28328180 @default.
• W2003011323 hasConcept C49039625 @default.
• W2003011323 hasConcept C54355233 @default.
• W2003011323 hasConcept C553184892 @default.
• W2003011323 hasConcept C55493867 @default.
• W2003011323 hasConcept C62478195 @default.
• W2003011323 hasConcept C81885089 @default.
• W2003011323 hasConcept C86803240 @default.
• W2003011323 hasConcept C880424 @default.
• W2003011323 hasConcept C95444343 @default.
• W2003011323 hasConceptScore W2003011323C104317684 @default.
• W2003011323 hasConceptScore W2003011323C108634530 @default.
• W2003011323 hasConceptScore W2003011323C109159458 @default.
• W2003011323 hasConceptScore W2003011323C134018914 @default.
• W2003011323 hasConceptScore W2003011323C150194340 @default.
• W2003011323 hasConceptScore W2003011323C153911025 @default.
• W2003011323 hasConceptScore W2003011323C177707886 @default.
• W2003011323 hasConceptScore W2003011323C17991360 @default.

• next