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• W2003012816 startingPage "245" @default.
• W2003012816 abstract "There is an increasing need for tissue-specific gene expression regulatory elements to study normal and disease development in the mouse. However, the cloning and characterization of these elements are time-consuming and costly. Thus, there is a particular need to be able to identify gene expression patterns without having to clone the promoter elements. Gene-trap strategies identify expression patterns assigned for endogenous genes using reporters, such as LacZ (Gossler et al., 1989; Skarnes, 1990) or green fluorescent protein (GFP) (Ishida and Leder, 1999; Zheng and Hughes, 1999). The gene-trap vector randomly inserts into the genome and steals regulatory elements for the reporter. Here we describe an improved gene-trap strategy, which allows an efficient Cre recombinase-mediated insertion of any transgene into the trapped loci as a post-integrational modification and links the expression of the transgene to that of the reporter." @default.
• W2003012816 created "2016-06-24" @default.
• W2003012816 creator A5033645768 @default.
• W2003012816 creator A5088539750 @default.
• W2003012816 date "2000-04-01" @default.
• W2003012816 modified "2023-09-26" @default.
• W2003012816 title "Gene-trap-based target site for Cre-mediated transgenic insertion" @default.
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• W2003012816 doi "https://doi.org/10.1002/(sici)1526-968x(200004)26:4<245::aid-gene50>3.0.co;2-9" @default.
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