Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003014014> ?p ?o ?g. }
- W2003014014 endingPage "e94285" @default.
- W2003014014 startingPage "e94285" @default.
- W2003014014 abstract "Enterohemorrhagic Escherichia coli (EHEC), an emerging food- and water-borne hazard, is highly pathogenic to humans. In the environment, EHEC must survive phosphate (Pi) limitation. The response to such Pi starvation is an induction of the Pho regulon including the Pst system that senses Pi variation. The interplay between the virulence of EHEC, Pho-Pst system and environmental Pi remains unknown. To understand the effects of Pi deprivation on the molecular mechanisms involved in EHEC survival and virulence under Pho regulon control, we undertook transcriptome profiling of the EDL933 wild-type strain grown under high Pi and low Pi conditions and its isogenic ΔphoB mutant grown in low Pi conditions. The differentially expressed genes included 1067 Pi-dependent genes and 603 PhoB-dependent genes. Of these 131 genes were both Pi and PhoB-dependent. Differentially expressed genes that were selected included those involved in Pi homeostasis, cellular metabolism, acid stress, oxidative stress and RpoS-dependent stress responses. Differentially expressed virulence systems included the locus of enterocyte effacement (LEE) encoding the type-3 secretion system (T3SS) and its effectors, as well as BP-933W prophage encoded Shiga toxin 2 genes. Moreover, PhoB directly regulated LEE and stx2 gene expression through binding to specific Pho boxes. However, in Pi-rich medium, constitutive activation of the Pho regulon decreased LEE gene expression and reduced adherence to HeLa cells. Together, these findings reveal that EHEC has evolved a sophisticated response to Pi limitation involving multiple biochemical strategies that contribute to its ability to respond to variations in environmental Pi and to coordinating the virulence response." @default.
- W2003014014 created "2016-06-24" @default.
- W2003014014 creator A5059182343 @default.
- W2003014014 creator A5065155909 @default.
- W2003014014 creator A5070180686 @default.
- W2003014014 creator A5075938981 @default.
- W2003014014 date "2014-04-07" @default.
- W2003014014 modified "2023-10-16" @default.
- W2003014014 title "PhoB Activates Escherichia coli O157:H7 Virulence Factors in Response to Inorganic Phosphate Limitation" @default.
- W2003014014 cites W142507464 @default.
- W2003014014 cites W1572646932 @default.
- W2003014014 cites W1762126222 @default.
- W2003014014 cites W1781531090 @default.
- W2003014014 cites W1786268878 @default.
- W2003014014 cites W1833844910 @default.
- W2003014014 cites W1850389663 @default.
- W2003014014 cites W1852859757 @default.
- W2003014014 cites W1871263052 @default.
- W2003014014 cites W1904815041 @default.
- W2003014014 cites W1958594228 @default.
- W2003014014 cites W1964868893 @default.
- W2003014014 cites W1965642694 @default.
- W2003014014 cites W1971023242 @default.
- W2003014014 cites W1975415672 @default.
- W2003014014 cites W1975818668 @default.
- W2003014014 cites W1976213625 @default.
- W2003014014 cites W1977681329 @default.
- W2003014014 cites W1979723665 @default.
- W2003014014 cites W1981521699 @default.
- W2003014014 cites W1982049511 @default.
- W2003014014 cites W1984136937 @default.
- W2003014014 cites W1985396638 @default.
- W2003014014 cites W1987573660 @default.
- W2003014014 cites W2001130452 @default.
- W2003014014 cites W2002388494 @default.
- W2003014014 cites W2003482560 @default.
- W2003014014 cites W2005500557 @default.
- W2003014014 cites W2006616848 @default.
- W2003014014 cites W2011842731 @default.
- W2003014014 cites W2022951217 @default.
- W2003014014 cites W2029104000 @default.
- W2003014014 cites W2029608133 @default.
- W2003014014 cites W2030640784 @default.
- W2003014014 cites W2031859095 @default.
- W2003014014 cites W2035877310 @default.
- W2003014014 cites W2035890664 @default.
- W2003014014 cites W2045131606 @default.
- W2003014014 cites W2048842389 @default.
- W2003014014 cites W2051347791 @default.
- W2003014014 cites W2057044419 @default.
- W2003014014 cites W2060415998 @default.
- W2003014014 cites W2072094007 @default.
- W2003014014 cites W2072098717 @default.
- W2003014014 cites W2072862102 @default.
- W2003014014 cites W2077672202 @default.
- W2003014014 cites W2083637418 @default.
- W2003014014 cites W2086893286 @default.
- W2003014014 cites W2093021888 @default.
- W2003014014 cites W2096070179 @default.
- W2003014014 cites W2096679244 @default.
- W2003014014 cites W2096786316 @default.
- W2003014014 cites W2102382756 @default.
- W2003014014 cites W2106702513 @default.
- W2003014014 cites W2114108202 @default.
- W2003014014 cites W2121480111 @default.
- W2003014014 cites W2123629406 @default.
- W2003014014 cites W2136309052 @default.
- W2003014014 cites W2139133542 @default.
- W2003014014 cites W2141396945 @default.
- W2003014014 cites W2145336455 @default.
- W2003014014 cites W2147776446 @default.
- W2003014014 cites W2150625556 @default.
- W2003014014 cites W2153531943 @default.
- W2003014014 cites W2154203929 @default.
- W2003014014 cites W2158266769 @default.
- W2003014014 cites W2158408911 @default.
- W2003014014 cites W2159177223 @default.
- W2003014014 cites W2164357021 @default.
- W2003014014 cites W2166761865 @default.
- W2003014014 cites W2169206272 @default.
- W2003014014 cites W2171120339 @default.
- W2003014014 cites W2234170532 @default.
- W2003014014 cites W2304027217 @default.
- W2003014014 cites W4248664566 @default.
- W2003014014 cites W60851034 @default.
- W2003014014 doi "https://doi.org/10.1371/journal.pone.0094285" @default.
- W2003014014 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3978041" @default.
- W2003014014 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24710330" @default.
- W2003014014 hasPublicationYear "2014" @default.
- W2003014014 type Work @default.
- W2003014014 sameAs 2003014014 @default.
- W2003014014 citedByCount "46" @default.
- W2003014014 countsByYear W20030140142014 @default.
- W2003014014 countsByYear W20030140142015 @default.
- W2003014014 countsByYear W20030140142016 @default.
- W2003014014 countsByYear W20030140142017 @default.
- W2003014014 countsByYear W20030140142018 @default.
- W2003014014 countsByYear W20030140142019 @default.