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- W2003015181 abstract "Abstract Based upon their resistance to irreversible denaturation, covalently closed circular (CCC) DNA (non-nicked, double-stranded circular molecules) can be purified by alkaline denaturation, neutralization, and filtration through a nitrocellulose membrane. This procedure offers a simple means of isolating in vitro synthesized CCC DNA molecules. The preparations of molecules obtained by this method consisted of 91-97% CCC DNA and contained no detectable inhibitors of biological activity or enzymatic digestion." @default.
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- W2003015181 title "Isolation of in Vitro Synthesized Covalently Closed Circular Double-Stranded DNA by Selective Denaturation and Filtration" @default.
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- W2003015181 doi "https://doi.org/10.1080/00327488408061782" @default.
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