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• W2003016263 abstract "Noncoding RNAs (ncRNAs) are important functional RNAs that do not code for proteins. We present a highly efficient computational pipeline for discovering cis-regulatory ncRNA motifs de novo. The pipeline differs from previous methods in that it is structure-oriented, does not require a multiple-sequence alignment as input, and is capable of detecting RNA motifs with low sequence conservation. We also integrate RNA motif prediction with RNA homolog search, which improves the quality of the RNA motifs significantly. Here, we report the results of applying this pipeline to Firmicute bacteria. Our top-ranking motifs include most known Firmicute elements found in the RNA family database (Rfam). Comparing our motif models with Rfam's hand-curated motif models, we achieve high accuracy in both membership prediction and base-pair–level secondary structure prediction (at least 75% average sensitivity and specificity on both tasks). Of the ncRNA candidates not in Rfam, we find compelling evidence that some of them are functional, and analyze several potential ribosomal protein leaders in depth." @default.
• W2003016263 created "2016-06-24" @default.
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• W2003016263 date "2007-07-06" @default.
• W2003016263 modified "2023-10-15" @default.
• W2003016263 title "A Computational Pipeline for High- Throughput Discovery of cis-Regulatory Noncoding RNA in Prokaryotes" @default.
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• W2003016263 doi "https://doi.org/10.1371/journal.pcbi.0030126" @default.
• W2003016263 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1913097" @default.
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