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- W2003024923 abstract "Synthetic Escherichia coli-type lipid A formed hexagonal plate crystals when it was precipitated by the addition of 2 volumes of 95% ethanol containing 375 mM MgCl2 and incubated in 70% ethanol containing 250 mM MgCl2 at 4 C for 10 days. Analyses of crystals by electron diffraction and synchrotron X-ray diffraction showed that crystals consist of hexagonal lattices with the lattice constant (a side of the lozenge as a unit cell on the basal plane) of 4.62 angstrom and the longitudinal axis (perpendicular to the basal plane) of 49.3 +/- 1.3 angstrom. Results suggest that the previous finding that various kinds of R-form lipopolysaccharides crystallized but free lipid A isolated by acid hydrolysis from Re lipopolysaccharide did not crystallize under the same experimental conditions (Kato et al, J. Bacteriol., 172:1516-1528, 1990) is due to structural changes of lipid A occurring during the procedure of isolation of free lipid A." @default.
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- W2003024923 date "1996-01-01" @default.
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- W2003024923 title "Crystallization of Synthetic<i>Escherichia coli</i>-Type Lipid A" @default.
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- W2003024923 doi "https://doi.org/10.1111/j.1348-0421.1996.tb03314.x" @default.
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