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- W2003027402 abstract "Abstract A selective reversed‐phase (RP) liquid chromatographic (LC) method coupled with UV for the determination of tylosin and its related substances is described. The gradient method uses a Capcell pak C18 ACR column (25 cm×4.6 mm id, 5 μm) maintained at a temperature of 60°C. The mobile phases consist of acetonitrile, phosphate buffer pH 5.5 and water: (A; 27.5:10:62.5 v/v/v) and (B; 50:10:40 v/v/v). The flow rate is 1.0 mL/min and UV detection is performed at 280 nm. It allows the separation of all known and 22 other unknown related substances (≥0.02%) from the main compound and from one another. The method shows good precision, sensitivity, linearity (between 0.2 μg/mL and 1.25 mg/mL) and robustness. The limit of quantification is 0.2 μg/mL, corresponding to 0.020%. Seven bulk tylosin samples containing a large number of impurities were examined using this method." @default.
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- W2003027402 date "2011-08-02" @default.
- W2003027402 modified "2023-10-18" @default.
- W2003027402 title "An improved liquid chromatographic method for the analysis of tylosin and its impurities" @default.
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- W2003027402 doi "https://doi.org/10.1002/jssc.201100340" @default.
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