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- W2003033924 abstract "A microtechnique is described for inducing IL-1 activity in vitro from frozen human blood mononuclear cells (BMNC) using the lymphocyte-activating factor (LAF) method. When compared to the conventional culture system, this micromethod offers several advantages: the complete assay requires a total of 1.0 X 10(5) mononuclear cells which is easily obtained from 1 ml of whole blood; the method of isolation and freezing of cells preserves their viability and ability to produce IL-1 for several weeks; when compared to the conventional test using fresh BMNC, frozen cells produce the same amount of IL-1. The microtechnique described here is highly reproducible and provides a useful tool for evaluating the production of IL-1 from BMNC of human subjects in clinical studies including those with immunodeficiencies, malnutrition and malignant diseases." @default.
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- W2003033924 date "1987-05-01" @default.
- W2003033924 modified "2023-09-27" @default.
- W2003033924 title "A new micromethod for determination of interleukin-1 production from frozen human blood mononuclear cells" @default.
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- W2003033924 doi "https://doi.org/10.1016/0022-1759(87)90040-8" @default.
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