FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003067503> ?p ?o ?g. }

• W2003067503 endingPage "5958" @default.
• W2003067503 startingPage "5947" @default.
• W2003067503 abstract "Immunodepletion of abundant plasma proteins increases the depth of proteome penetration by mass spectrometry. However, the nature and extent of immunodepletion and the effect of off-target depletion on the quantitative comparison of the residual proteins have not been critically addressed. We performed mass spectrometry label-free quantitation to determine which proteins were immunodepleted and by how much. Two immunodepletion resins were compared: Qproteome (Qiagen) which removes albumin + immunoglobulins and Seppro IgY14 + SuperMix (Sigma-Aldrich) which removes 14 target proteins plus a number of unidentified proteins. Plasma collected by P100 proteomic plasma collection tubes (BD) from 20 human subjects was individually immunodepleted to minimize potential variability, prior to pooling. The abundant proteins were quantified better when using only albumin + immunoglobulins removal (Qproteome), while lower abundance proteins were evaluated better using exhaustive immunodepletion (Seppro IgY14 + SuperMix). The latter resin removed at least 155 proteins, 38% of the plasma proteome in protein number and 94% of plasma protein in mass. The depth of immunodepletion likely accounts for the effectiveness of this resin in revealing low abundance proteins. However, the more profound immunodepletion achieved with the IgY14 + SuperMix may lead to false-positive fold-changes between comparison groups if the reproducibility and efficiency of the depletion of a given protein are not considered." @default.
• W2003067503 created "2016-06-24" @default.
• W2003067503 creator A5000922837 @default.
• W2003067503 creator A5004331456 @default.
• W2003067503 creator A5017665414 @default.
• W2003067503 creator A5033355631 @default.
• W2003067503 creator A5036224463 @default.
• W2003067503 creator A5042236983 @default.
• W2003067503 creator A5057312365 @default.
• W2003067503 creator A5069206173 @default.
• W2003067503 creator A5069273632 @default.
• W2003067503 creator A5074783469 @default.
• W2003067503 date "2012-10-29" @default.
• W2003067503 modified "2023-09-28" @default.
• W2003067503 title "Assessment of Two Immunodepletion Methods: Off-Target Effects and Variations in Immunodepletion Efficiency May Confound Plasma Proteomics" @default.
• W2003067503 cites W1969917966 @default.
• W2003067503 cites W1975586699 @default.
• W2003067503 cites W1980311420 @default.
• W2003067503 cites W1983335555 @default.
• W2003067503 cites W1983470047 @default.
• W2003067503 cites W1987204908 @default.
• W2003067503 cites W1998126392 @default.
• W2003067503 cites W2017632081 @default.
• W2003067503 cites W2020707691 @default.
• W2003067503 cites W2021273025 @default.
• W2003067503 cites W2027776285 @default.
• W2003067503 cites W2032894305 @default.
• W2003067503 cites W2045511831 @default.
• W2003067503 cites W2045964900 @default.
• W2003067503 cites W2046095066 @default.
• W2003067503 cites W2053988331 @default.
• W2003067503 cites W2067677548 @default.
• W2003067503 cites W2076248459 @default.
• W2003067503 cites W2082119649 @default.
• W2003067503 cites W2082907106 @default.
• W2003067503 cites W2087746647 @default.
• W2003067503 cites W2115634458 @default.
• W2003067503 cites W2121719876 @default.
• W2003067503 cites W2146514656 @default.
• W2003067503 cites W2147786371 @default.
• W2003067503 cites W2148490163 @default.
• W2003067503 cites W2162655753 @default.
• W2003067503 doi "https://doi.org/10.1021/pr300686k" @default.
• W2003067503 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3518753" @default.
• W2003067503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23082855" @default.
• W2003067503 hasPublicationYear "2012" @default.
• W2003067503 type Work @default.
• W2003067503 sameAs 2003067503 @default.
• W2003067503 citedByCount "44" @default.
• W2003067503 countsByYear W20030675032013 @default.
• W2003067503 countsByYear W20030675032014 @default.
• W2003067503 countsByYear W20030675032015 @default.
• W2003067503 countsByYear W20030675032016 @default.
• W2003067503 countsByYear W20030675032017 @default.
• W2003067503 countsByYear W20030675032018 @default.
• W2003067503 countsByYear W20030675032019 @default.
• W2003067503 countsByYear W20030675032020 @default.
• W2003067503 countsByYear W20030675032021 @default.
• W2003067503 countsByYear W20030675032022 @default.
• W2003067503 countsByYear W20030675032023 @default.
• W2003067503 crossrefType "journal-article" @default.
• W2003067503 hasAuthorship W2003067503A5000922837 @default.
• W2003067503 hasAuthorship W2003067503A5004331456 @default.
• W2003067503 hasAuthorship W2003067503A5017665414 @default.
• W2003067503 hasAuthorship W2003067503A5033355631 @default.
• W2003067503 hasAuthorship W2003067503A5036224463 @default.
• W2003067503 hasAuthorship W2003067503A5042236983 @default.
• W2003067503 hasAuthorship W2003067503A5057312365 @default.
• W2003067503 hasAuthorship W2003067503A5069206173 @default.
• W2003067503 hasAuthorship W2003067503A5069273632 @default.
• W2003067503 hasAuthorship W2003067503A5074783469 @default.
• W2003067503 hasBestOaLocation W20030675032 @default.
• W2003067503 hasConcept C104317684 @default.
• W2003067503 hasConcept C104397665 @default.
• W2003067503 hasConcept C146543888 @default.
• W2003067503 hasConcept C153911025 @default.
• W2003067503 hasConcept C162356407 @default.
• W2003067503 hasConcept C185592680 @default.
• W2003067503 hasConcept C2776125364 @default.
• W2003067503 hasConcept C3020134792 @default.
• W2003067503 hasConcept C43617362 @default.
• W2003067503 hasConcept C46111723 @default.
• W2003067503 hasConcept C55493867 @default.
• W2003067503 hasConcept C80311884 @default.
• W2003067503 hasConcept C86803240 @default.
• W2003067503 hasConceptScore W2003067503C104317684 @default.
• W2003067503 hasConceptScore W2003067503C104397665 @default.
• W2003067503 hasConceptScore W2003067503C146543888 @default.
• W2003067503 hasConceptScore W2003067503C153911025 @default.
• W2003067503 hasConceptScore W2003067503C162356407 @default.
• W2003067503 hasConceptScore W2003067503C185592680 @default.
• W2003067503 hasConceptScore W2003067503C2776125364 @default.
• W2003067503 hasConceptScore W2003067503C3020134792 @default.
• W2003067503 hasConceptScore W2003067503C43617362 @default.
• W2003067503 hasConceptScore W2003067503C46111723 @default.
• W2003067503 hasConceptScore W2003067503C55493867 @default.
• W2003067503 hasConceptScore W2003067503C80311884 @default.
• W2003067503 hasConceptScore W2003067503C86803240 @default.

• next