FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003075395> ?p ?o ?g. }

• W2003075395 endingPage "212" @default.
• W2003075395 startingPage "205" @default.
• W2003075395 abstract "Recombinant DNA plasmids coding for transforming growth factor beta 2 (TGF-beta 2) precursor and a hybrid TGF-beta 1(NH2)/beta 2(COOH) molecule consisting of the amino-terminal precursor portion of transforming growth factor-beta 1 (TGF-beta 1) linked in phase to the carboxyl terminus of mature TGF-beta 2 were constructed and transfected into COS cells. Both plasmids directed the synthesis of active TGF-beta 2 which was secreted into the supernatants of transfected cells. The TGF-beta 2 was secreted in a latent form, as an acidification step was required to demonstrate optimal biological activity. Using site-specific anti-peptide antibodies, we show that precursor and mature forms of TGF-beta 2 are produced. A stable Chinese hamster ovary (CHO) cell line expressing the hybrid TGF-beta 1(NH2)/beta 2(COOH) protein was isolated. This cell line secreted both precursor and mature forms of TGF-beta 1(NH2)/beta 2(COOH); acidification was required to demonstrate biological activity. Protein sequence analysis of recombinant TGF-beta 2 produced by this CHO clone demonstrated that correct proteolytic cleavage had occurred, suggesting that the processing signals contained within the TGF-beta 1 amino portion can function in producing mature TGF-beta 2. Receptor binding studies showed that TGF-beta 2 specifically bound predominantly to type III receptors on the surface of human palatal mesenchymal cells. The availability of active TGF-beta 2 should aid in determining its potential therapeutic use as a growth modulator." @default.
• W2003075395 created "2016-06-24" @default.
• W2003075395 creator A5010767401 @default.
• W2003075395 creator A5014258445 @default.
• W2003075395 creator A5059191263 @default.
• W2003075395 creator A5071112218 @default.
• W2003075395 creator A5071978239 @default.
• W2003075395 creator A5077754616 @default.
• W2003075395 creator A5082058844 @default.
• W2003075395 creator A5086083160 @default.
• W2003075395 date "1989-04-01" @default.
• W2003075395 modified "2023-09-23" @default.
• W2003075395 title "Expression and Characterization of Recombinant TGF-β2 Proteins Produced in Mammalian Cells" @default.
• W2003075395 cites W143417350 @default.
• W2003075395 cites W1480727199 @default.
• W2003075395 cites W1488959490 @default.
• W2003075395 cites W1495519011 @default.
• W2003075395 cites W1500321338 @default.
• W2003075395 cites W1500595986 @default.
• W2003075395 cites W1505978562 @default.
• W2003075395 cites W1515313654 @default.
• W2003075395 cites W1524114372 @default.
• W2003075395 cites W1528585209 @default.
• W2003075395 cites W1595226323 @default.
• W2003075395 cites W1600868878 @default.
• W2003075395 cites W1608791497 @default.
• W2003075395 cites W1660743658 @default.
• W2003075395 cites W1894312995 @default.
• W2003075395 cites W1963932192 @default.
• W2003075395 cites W1968997237 @default.
• W2003075395 cites W1969713014 @default.
• W2003075395 cites W1973644988 @default.
• W2003075395 cites W1974967880 @default.
• W2003075395 cites W1979877257 @default.
• W2003075395 cites W1987408592 @default.
• W2003075395 cites W1990311250 @default.
• W2003075395 cites W1992172901 @default.
• W2003075395 cites W1996623331 @default.
• W2003075395 cites W2002946136 @default.
• W2003075395 cites W2007768242 @default.
• W2003075395 cites W2017141333 @default.
• W2003075395 cites W2050620572 @default.
• W2003075395 cites W2050687675 @default.
• W2003075395 cites W2052589062 @default.
• W2003075395 cites W2055296386 @default.
• W2003075395 cites W2066525457 @default.
• W2003075395 cites W2073473087 @default.
• W2003075395 cites W2081689397 @default.
• W2003075395 cites W2083093986 @default.
• W2003075395 cites W2084099638 @default.
• W2003075395 cites W2087863947 @default.
• W2003075395 cites W2094064497 @default.
• W2003075395 cites W2111817213 @default.
• W2003075395 cites W2118857294 @default.
• W2003075395 cites W2119209023 @default.
• W2003075395 cites W2144464927 @default.
• W2003075395 cites W2161454032 @default.
• W2003075395 cites W2170395027 @default.
• W2003075395 cites W2336518283 @default.
• W2003075395 cites W4229906000 @default.
• W2003075395 cites W4239324416 @default.
• W2003075395 doi "https://doi.org/10.1089/dna.1.1989.8.205" @default.
• W2003075395 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2721369" @default.
• W2003075395 hasPublicationYear "1989" @default.
• W2003075395 type Work @default.
• W2003075395 sameAs 2003075395 @default.
• W2003075395 citedByCount "25" @default.
• W2003075395 countsByYear W20030753952017 @default.
• W2003075395 countsByYear W20030753952019 @default.
• W2003075395 crossrefType "journal-article" @default.
• W2003075395 hasAuthorship W2003075395A5010767401 @default.
• W2003075395 hasAuthorship W2003075395A5014258445 @default.
• W2003075395 hasAuthorship W2003075395A5059191263 @default.
• W2003075395 hasAuthorship W2003075395A5071112218 @default.
• W2003075395 hasAuthorship W2003075395A5071978239 @default.
• W2003075395 hasAuthorship W2003075395A5077754616 @default.
• W2003075395 hasAuthorship W2003075395A5082058844 @default.
• W2003075395 hasAuthorship W2003075395A5086083160 @default.
• W2003075395 hasConcept C10205521 @default.
• W2003075395 hasConcept C104317684 @default.
• W2003075395 hasConcept C111001777 @default.
• W2003075395 hasConcept C118131993 @default.
• W2003075395 hasConcept C125555471 @default.
• W2003075395 hasConcept C153911025 @default.
• W2003075395 hasConcept C167625842 @default.
• W2003075395 hasConcept C170493617 @default.
• W2003075395 hasConcept C171020997 @default.
• W2003075395 hasConcept C175656101 @default.
• W2003075395 hasConcept C22744801 @default.
• W2003075395 hasConcept C2775960820 @default.
• W2003075395 hasConcept C2778229498 @default.
• W2003075395 hasConcept C2781381406 @default.
• W2003075395 hasConcept C28328180 @default.
• W2003075395 hasConcept C40767141 @default.
• W2003075395 hasConcept C54009773 @default.
• W2003075395 hasConcept C54355233 @default.
• W2003075395 hasConcept C55493867 @default.
• W2003075395 hasConcept C6061869 @default.

• next