FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003113535> ?p ?o ?g. }

• W2003113535 endingPage "502" @default.
• W2003113535 startingPage "495" @default.
• W2003113535 abstract "ABSTRACT Nodamura virus (NoV) and Flock House virus (FHV) are members of the family Nodaviridae . The nodavirus genome is composed of two positive-sense RNA segments: RNA1 encodes the viral RNA-dependent RNA polymerase and RNA2 encodes the capsid protein precursor. A small subgenomic RNA3, which encodes nonstructural proteins B1 and B2, is transcribed from RNA1 during RNA replication. Previously, FHV was shown to replicate both of its genomic RNAs and to transcribe RNA3 in transiently transfected yeast cells. FHV RNAs and their derivatives could also be expressed from plasmids containing RNA polymerase II promoters. Here we show that all of these features can be recapitulated for NoV, the only nodavirus that productively infects mammals. Inducible plasmid-based systems were used to characterize the RNA replication requirements for NoV RNA1 and RNA2 in Saccharomyces cerevisiae . Induced NoV RNA1 replication was robust. Three previously described NoV RNA1 mutants behaved in yeast as they had in mammalian cells. Yeast colonies were selected from cells expressing NoV RNA1, and RNA2 replicons that encoded yeast nutritional markers, from plasmids. Unexpectedly, these NoV RNA replication-dependent yeast colonies were recovered at frequencies 10 4 -fold lower than in the analogous FHV system. Molecular analysis revealed that some of the NoV RNA replication-dependent colonies contained mutations in the NoV B2 open reading frame in the replicating viral RNA. In addition, we found that NoV RNA1 could support limited replication of a deletion derivative of the heterologous FHV RNA2 that expressed the yeast HIS3 selectable marker, resulting in formation of HIS + colonies." @default.
• W2003113535 created "2016-06-24" @default.
• W2003113535 creator A5005163092 @default.
• W2003113535 creator A5026819884 @default.
• W2003113535 creator A5064173141 @default.
• W2003113535 creator A5075193538 @default.
• W2003113535 date "2005-01-01" @default.
• W2003113535 modified "2023-09-23" @default.
• W2003113535 title "Nodamura Virus RNA Replication in <i>Saccharomyces cerevisiae</i> : Heterologous Gene Expression Allows Replication-Dependent Colony Formation" @default.
• W2003113535 cites W1487448001 @default.
• W2003113535 cites W1522217335 @default.
• W2003113535 cites W1966323687 @default.
• W2003113535 cites W1967390330 @default.
• W2003113535 cites W1967516118 @default.
• W2003113535 cites W1971317761 @default.
• W2003113535 cites W1992687669 @default.
• W2003113535 cites W1993084380 @default.
• W2003113535 cites W2012288774 @default.
• W2003113535 cites W2018802169 @default.
• W2003113535 cites W2022068951 @default.
• W2003113535 cites W2025095545 @default.
• W2003113535 cites W2035132867 @default.
• W2003113535 cites W2058147071 @default.
• W2003113535 cites W2058426472 @default.
• W2003113535 cites W2061741217 @default.
• W2003113535 cites W2081090611 @default.
• W2003113535 cites W2099013587 @default.
• W2003113535 cites W2103984261 @default.
• W2003113535 cites W2105893559 @default.
• W2003113535 cites W2106783844 @default.
• W2003113535 cites W2115073295 @default.
• W2003113535 cites W2117869267 @default.
• W2003113535 cites W2125119690 @default.
• W2003113535 cites W2135659255 @default.
• W2003113535 cites W2139268538 @default.
• W2003113535 cites W2146503498 @default.
• W2003113535 cites W2165990041 @default.
• W2003113535 cites W2166293484 @default.
• W2003113535 cites W2172168549 @default.
• W2003113535 cites W2196827467 @default.
• W2003113535 cites W2404517092 @default.
• W2003113535 doi "https://doi.org/10.1128/jvi.79.1.495-502.2005" @default.
• W2003113535 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/538723" @default.
• W2003113535 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15596842" @default.
• W2003113535 hasPublicationYear "2005" @default.
• W2003113535 type Work @default.
• W2003113535 sameAs 2003113535 @default.
• W2003113535 citedByCount "28" @default.
• W2003113535 countsByYear W20031135352012 @default.
• W2003113535 countsByYear W20031135352013 @default.
• W2003113535 countsByYear W20031135352014 @default.
• W2003113535 countsByYear W20031135352015 @default.
• W2003113535 countsByYear W20031135352017 @default.
• W2003113535 countsByYear W20031135352018 @default.
• W2003113535 countsByYear W20031135352020 @default.
• W2003113535 countsByYear W20031135352021 @default.
• W2003113535 countsByYear W20031135352022 @default.
• W2003113535 crossrefType "journal-article" @default.
• W2003113535 hasAuthorship W2003113535A5005163092 @default.
• W2003113535 hasAuthorship W2003113535A5026819884 @default.
• W2003113535 hasAuthorship W2003113535A5064173141 @default.
• W2003113535 hasAuthorship W2003113535A5075193538 @default.
• W2003113535 hasBestOaLocation W20031135352 @default.
• W2003113535 hasConcept C104317684 @default.
• W2003113535 hasConcept C140704245 @default.
• W2003113535 hasConcept C153911025 @default.
• W2003113535 hasConcept C159047783 @default.
• W2003113535 hasConcept C18103114 @default.
• W2003113535 hasConcept C22744801 @default.
• W2003113535 hasConcept C2522874641 @default.
• W2003113535 hasConcept C41258723 @default.
• W2003113535 hasConcept C54355233 @default.
• W2003113535 hasConcept C58337764 @default.
• W2003113535 hasConcept C67705224 @default.
• W2003113535 hasConcept C86803240 @default.
• W2003113535 hasConceptScore W2003113535C104317684 @default.
• W2003113535 hasConceptScore W2003113535C140704245 @default.
• W2003113535 hasConceptScore W2003113535C153911025 @default.
• W2003113535 hasConceptScore W2003113535C159047783 @default.
• W2003113535 hasConceptScore W2003113535C18103114 @default.
• W2003113535 hasConceptScore W2003113535C22744801 @default.
• W2003113535 hasConceptScore W2003113535C2522874641 @default.
• W2003113535 hasConceptScore W2003113535C41258723 @default.
• W2003113535 hasConceptScore W2003113535C54355233 @default.
• W2003113535 hasConceptScore W2003113535C58337764 @default.
• W2003113535 hasConceptScore W2003113535C67705224 @default.
• W2003113535 hasConceptScore W2003113535C86803240 @default.
• W2003113535 hasIssue "1" @default.
• W2003113535 hasLocation W20031135351 @default.
• W2003113535 hasLocation W20031135352 @default.
• W2003113535 hasLocation W20031135353 @default.
• W2003113535 hasLocation W20031135354 @default.
• W2003113535 hasOpenAccess W2003113535 @default.
• W2003113535 hasPrimaryLocation W20031135351 @default.
• W2003113535 hasRelatedWork W1956881088 @default.
• W2003113535 hasRelatedWork W1963482194 @default.
• W2003113535 hasRelatedWork W1967266223 @default.
• W2003113535 hasRelatedWork W1969881112 @default.

• next