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- W2003113607 abstract "The transforming growth factor-β1 (TGF-β1) bioassay developed in this study monitors increased luciferase activity in MCF10A cells containing the matrix metalloproteinase-2 (MMP-2) promoter with a luciferase reporter and treated with increasing TGF-β1 concentrations. The response was linear in the concentration range from 75 to 2,500 pg/mL. The abilities of 3 types of TGF-β in inducing MMP-2 were different. The luciferase activity induced by TGF-β1 was about 2 times more than that by TGF-β2 and TGF-β3. The MMP-2 promoter bioassay showed greater reproducibility (coefficient of variation [CV] 10%) than the previously developed anticell proliferation assay of TF-1 cell (CV 16%) and the MMP-2 zymogram assay (CV 40%)." @default.
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- W2003113607 date "2010-09-01" @default.
- W2003113607 modified "2023-09-24" @default.
- W2003113607 title "Transforming Growth Factor-β1 Bioassay Involving Matrix Metalloproteinase-2 Induction" @default.
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- W2003113607 doi "https://doi.org/10.1089/jir.2009.0058" @default.
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