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- W2003114206 abstract "We developed an experimental approach to measure somatic and ciliary Cat2+-signals in the ciliate Didinium under voltage clamp conditions using the dye Fura-2. Intracellular pressure injection of Fura-2 molecules did not alter electrophysiological membrane properties besides an expected buffering effect. The intracellular free Ca2+-concentration was determined at 2 × 10−7 M. During membrane excitation, this resting value increased in the cilia; a quantification was not feasible. Within the cell soma, however, the Ca2+-level was unchanged within the physiological range of the membrane potential (-70 mV to 0 mV). Increasing the driving force for Ca2+-ions via strong hyperpolarization (potentials negative to −200 mV) a centripetal increase in the somatic Ca2+ concentration was found. Our results support the hypothesis that Ca2+ is the intracellular messenger in rapid electromotor coupling in ciliates." @default.
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- W2003114206 title "Fluorometric measurement of the intracellular free Ca2+-concentration in the ciliate Didinium nasutum using Fura-2" @default.
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- W2003114206 doi "https://doi.org/10.1016/0143-4160(95)90011-x" @default.
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