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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003117360> ?p ?o ?g. }

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• W2003117360 abstract "We describe here the properties of calcium green-5N (CG5N), a novel Ca2+-sensitive fluorescent probe. CG5N binds Ca2+ with an affinity of 4.29 ± 0.67 μM. Ca2+ binding is associated with a 14.7-fold increase in fluorescence. Mg2+ binds to CG5N with an affinity of 5 mM but produces a very small change in fluorescence. Using CG5N [Ca2+]i was recorded in single cultured neurons from fetal rat forebrain. Glutamate together with glycine increased [Ca2+]i by 1.17 ± 0.15 μM. Pretreating cells with 5 mM potassium cyanide significantly potentiated the response to glutamate, resulting in a mean increase of 8.8 ± 2 μM. These results demonstrate that CG5N is a useful indicator for measuring very large changes in [Ca2+]i in neurons." @default.
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• W2003117360 title "Calcium green-5N, a novel fluorescent probe for monitoring high intracellular free Ca2+ concentrations associated with glutamate excitotoxicity in cultured rat brain neurons" @default.
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• W2003117360 doi "https://doi.org/10.1016/0304-3940(93)90582-6" @default.
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