FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003126032> ?p ?o ?g. }

• W2003126032 endingPage "61" @default.
• W2003126032 startingPage "57" @default.
• W2003126032 abstract "A miniaturized two-dimensional gel electrophoresis (2-DE) system was constructed with a multifilament-supporting (MFS) gel as a first-dimensional gel for isoelectric focusing (IEF). The MFS gel was 1 mm in diameter, had a multifilament yarn of acrylic as a gel-support, and could provide low-concentration (2.5%T) polyacrylamide with sufficient mechanical strength, resulting in fast IEF, and clear 2-D spots due to the relatively large pore and improved transfer efficiency from the first-dimensional gel to the second-dimensional one of especially high-molecular-weight (HMW) proteins. In this study, by using the MFS gel of 4 cm in length as a miniaturized IEF gel, three colored proteins were separated in 8 min, which was about twice faster than using the MFS gel of standard length (7 cm). The second-dimensional gel was also miniaturized to be 55 mm wide, 40 mm long and 0.75 mm thick. The low-concentration MFS gel after IEF was easily transferred onto the top of the miniaturized second-dimensional gel with a tweezers. By using the miniaturized 2-DE system, the HMW size marker was separated in 30 min by IEF and in 25 min by native-polyacrylamide gel electrophoresis (Native-PAGE), which was at least twice faster than by using the standard size 2-DE system using MFS gel. This miniaturized 2-DE system could be expected as a useful separation method for fast protein diagnosis and screening." @default.
• W2003126032 created "2016-06-24" @default.
• W2003126032 creator A5007372143 @default.
• W2003126032 creator A5022244360 @default.
• W2003126032 creator A5022640967 @default.
• W2003126032 creator A5027721037 @default.
• W2003126032 creator A5030004320 @default.
• W2003126032 creator A5042075502 @default.
• W2003126032 creator A5043124263 @default.
• W2003126032 creator A5043265521 @default.
• W2003126032 creator A5061908845 @default.
• W2003126032 creator A5072235259 @default.
• W2003126032 date "2009-01-01" @default.
• W2003126032 modified "2023-10-03" @default.
• W2003126032 title "Miniaturized two-dimensional gel electrophoresis of high-molecular-weight proteins using low-concentration multifilament-supporting gel for isoelectric focusing" @default.
• W2003126032 cites W1964635334 @default.
• W2003126032 cites W1969763766 @default.
• W2003126032 cites W1972957068 @default.
• W2003126032 cites W1976866849 @default.
• W2003126032 cites W1978134082 @default.
• W2003126032 cites W2034676063 @default.
• W2003126032 cites W2051053105 @default.
• W2003126032 cites W2055197835 @default.
• W2003126032 cites W2079485569 @default.
• W2003126032 cites W2113874481 @default.
• W2003126032 cites W2125417135 @default.
• W2003126032 cites W2167518503 @default.
• W2003126032 cites W2478134988 @default.
• W2003126032 doi "https://doi.org/10.2198/jelectroph.53.57" @default.
• W2003126032 hasPublicationYear "2009" @default.
• W2003126032 type Work @default.
• W2003126032 sameAs 2003126032 @default.
• W2003126032 citedByCount "0" @default.
• W2003126032 crossrefType "journal-article" @default.
• W2003126032 hasAuthorship W2003126032A5007372143 @default.
• W2003126032 hasAuthorship W2003126032A5022244360 @default.
• W2003126032 hasAuthorship W2003126032A5022640967 @default.
• W2003126032 hasAuthorship W2003126032A5027721037 @default.
• W2003126032 hasAuthorship W2003126032A5030004320 @default.
• W2003126032 hasAuthorship W2003126032A5042075502 @default.
• W2003126032 hasAuthorship W2003126032A5043124263 @default.
• W2003126032 hasAuthorship W2003126032A5043265521 @default.
• W2003126032 hasAuthorship W2003126032A5061908845 @default.
• W2003126032 hasAuthorship W2003126032A5072235259 @default.
• W2003126032 hasBestOaLocation W20031260321 @default.
• W2003126032 hasConcept C101660505 @default.
• W2003126032 hasConcept C104317684 @default.
• W2003126032 hasConcept C113196181 @default.
• W2003126032 hasConcept C116084860 @default.
• W2003126032 hasConcept C131506403 @default.
• W2003126032 hasConcept C135763542 @default.
• W2003126032 hasConcept C152862880 @default.
• W2003126032 hasConcept C178596410 @default.
• W2003126032 hasConcept C181199279 @default.
• W2003126032 hasConcept C185592680 @default.
• W2003126032 hasConcept C192562407 @default.
• W2003126032 hasConcept C35859425 @default.
• W2003126032 hasConcept C40250595 @default.
• W2003126032 hasConcept C42084846 @default.
• W2003126032 hasConcept C43617362 @default.
• W2003126032 hasConcept C46111723 @default.
• W2003126032 hasConcept C47793336 @default.
• W2003126032 hasConcept C50476208 @default.
• W2003126032 hasConcept C55493867 @default.
• W2003126032 hasConceptScore W2003126032C101660505 @default.
• W2003126032 hasConceptScore W2003126032C104317684 @default.
• W2003126032 hasConceptScore W2003126032C113196181 @default.
• W2003126032 hasConceptScore W2003126032C116084860 @default.
• W2003126032 hasConceptScore W2003126032C131506403 @default.
• W2003126032 hasConceptScore W2003126032C135763542 @default.
• W2003126032 hasConceptScore W2003126032C152862880 @default.
• W2003126032 hasConceptScore W2003126032C178596410 @default.
• W2003126032 hasConceptScore W2003126032C181199279 @default.
• W2003126032 hasConceptScore W2003126032C185592680 @default.
• W2003126032 hasConceptScore W2003126032C192562407 @default.
• W2003126032 hasConceptScore W2003126032C35859425 @default.
• W2003126032 hasConceptScore W2003126032C40250595 @default.
• W2003126032 hasConceptScore W2003126032C42084846 @default.
• W2003126032 hasConceptScore W2003126032C43617362 @default.
• W2003126032 hasConceptScore W2003126032C46111723 @default.
• W2003126032 hasConceptScore W2003126032C47793336 @default.
• W2003126032 hasConceptScore W2003126032C50476208 @default.
• W2003126032 hasConceptScore W2003126032C55493867 @default.
• W2003126032 hasIssue "4" @default.
• W2003126032 hasLocation W20031260321 @default.
• W2003126032 hasOpenAccess W2003126032 @default.
• W2003126032 hasPrimaryLocation W20031260321 @default.
• W2003126032 hasRelatedWork W1555405795 @default.
• W2003126032 hasRelatedWork W1889243003 @default.
• W2003126032 hasRelatedWork W1974280078 @default.
• W2003126032 hasRelatedWork W2016420326 @default.
• W2003126032 hasRelatedWork W2023528486 @default.
• W2003126032 hasRelatedWork W2027963807 @default.
• W2003126032 hasRelatedWork W2034676063 @default.
• W2003126032 hasRelatedWork W2057025663 @default.
• W2003126032 hasRelatedWork W2090417131 @default.
• W2003126032 hasRelatedWork W2099568843 @default.
• W2003126032 hasRelatedWork W2146738896 @default.

• next