FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003137060> ?p ?o ?g. }

• W2003137060 endingPage "2297" @default.
• W2003137060 startingPage "2285" @default.
• W2003137060 abstract "The introduction of cloned T cell receptor (TCR) genes into bone marrow cells could provide a way to increase the frequency of tumor- or pathogen-specific cytotoxic T lymphocyte (CTL) precursors. We demonstrate here the ability of a retroviral vector to direct expression of a Vα15/Vβ13 MHC class I-restricted TCR in lethally irradiated mice reconstituted with transduced bone marrow cells. We have detected retroviral-mediated TCR expression by flow cytometry 6–19 weeks after transplantation in C57L (Vβ13¯/¯) and Rag1¯/¯ bone marrow-reconstituted mice, and in C57BL/6 hosts reconstituted with transduced C57BL/6-Rag1¯/¯bone marrow. Southern analysis confirmed the presence of integrated provirus and revealed that the frequency of transduction is greater than the frequency of cell surface TCR expression. Although TCR expression on Vβ13+ transduced cells is lower than endogenous TCR levels, it is largely confined to CD4+CD8+ (thymus) and CD8+ (thymus and spleen) T cells. In Rag1¯/¯ mice, which display a developmental arrest of thymocytes at the immature CD4¯CD8¯ stage, retrovirus-mediated TCR expression selectively rescues CD4+CD8+ and CD8+ populations. These results indicate that the ectopically expressed TCR is functional during T cell development. Furthermore, we have observed Vβ13+ TCR expression by up to 13% of peripheral CD8+ T cells in C57L and C57BL/6 hosts. This represents a substantial increase relative to total Vβ13 frequency in normal C57BL/6 mice (3–5%), and an even greater increase over the estimated frequency of CTL precursors of a defined specificity (10¯5–10¯4). Our findings indicate that TCR gene transfer can be used to develop new approaches to immunotherapy, and provide the basis for further studies examining the contribution of retrovirus-mediated TCR expression to an antigen-specific CTL response. Adoptive transfer of tumor-specific CTL contributes to tumor regression in a number of model systems. The transfer of genes encoding tumor-specific α/β TCR into hematopoietic stem cells could provide a valuable extension of this approach, particularly for malignancies treated with bone marrow transplantation and myeloablative doses of radiation or chemotherapy. We have previously constructed a retroviral vector that contains α- and β-chain genes encoding a mouse class 1 MHC-restricted TCR and demonstrated its ability to direct TCR expression in T hybridomas. We describe here the use of this vector to express the cloned TCR in T lymphoid cells of lethally irradiated mice reconstituted with transduced bone marrow cells. We also provide evidence that the ectopically expressed TCR is functional during T cell ontogeny, resulting in its selective expression by CD8+ T cells. The cassette design of our vector system allows insertion of PCR-generated V region cDNA fragments from any TCR, offering significant potential for the development of mouse models to evaluate TCR gene transfer into hematopoietic stem cells as a potential immunotherapeutic strategy." @default.
• W2003137060 created "2016-06-24" @default.
• W2003137060 creator A5028069544 @default.
• W2003137060 creator A5060299347 @default.
• W2003137060 creator A5069819571 @default.
• W2003137060 date "1998-10-10" @default.
• W2003137060 modified "2023-10-16" @default.
• W2003137060 title "Retroviral-Mediated Expression of an MHC Class I-Restricted T Cell Receptor in the CD8 T Cell Compartment of Bone Marrow-Reconstituted Mice" @default.
• W2003137060 cites W1516358214 @default.
• W2003137060 cites W1606253967 @default.
• W2003137060 cites W1964964271 @default.
• W2003137060 cites W1983514779 @default.
• W2003137060 cites W1992880129 @default.
• W2003137060 cites W1993702378 @default.
• W2003137060 cites W1996247810 @default.
• W2003137060 cites W2007038853 @default.
• W2003137060 cites W2007118925 @default.
• W2003137060 cites W2008634652 @default.
• W2003137060 cites W2016120395 @default.
• W2003137060 cites W2016520959 @default.
• W2003137060 cites W2033488512 @default.
• W2003137060 cites W2038558255 @default.
• W2003137060 cites W2045027597 @default.
• W2003137060 cites W2053627414 @default.
• W2003137060 cites W2058682689 @default.
• W2003137060 cites W2059809122 @default.
• W2003137060 cites W2068752450 @default.
• W2003137060 cites W2074327759 @default.
• W2003137060 cites W2094657382 @default.
• W2003137060 cites W2104785707 @default.
• W2003137060 cites W2107975432 @default.
• W2003137060 cites W2111759041 @default.
• W2003137060 cites W2118803516 @default.
• W2003137060 cites W2138403036 @default.
• W2003137060 cites W2140489687 @default.
• W2003137060 cites W2162508156 @default.
• W2003137060 cites W310472318 @default.
• W2003137060 cites W4239482503 @default.
• W2003137060 doi "https://doi.org/10.1089/hum.1998.9.15-2285" @default.
• W2003137060 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9794212" @default.
• W2003137060 hasPublicationYear "1998" @default.
• W2003137060 type Work @default.
• W2003137060 sameAs 2003137060 @default.
• W2003137060 citedByCount "7" @default.
• W2003137060 countsByYear W20031370602013 @default.
• W2003137060 crossrefType "journal-article" @default.
• W2003137060 hasAuthorship W2003137060A5028069544 @default.
• W2003137060 hasAuthorship W2003137060A5060299347 @default.
• W2003137060 hasAuthorship W2003137060A5069819571 @default.
• W2003137060 hasConcept C104317684 @default.
• W2003137060 hasConcept C113646796 @default.
• W2003137060 hasConcept C147969180 @default.
• W2003137060 hasConcept C153911025 @default.
• W2003137060 hasConcept C154317977 @default.
• W2003137060 hasConcept C156695909 @default.
• W2003137060 hasConcept C167672396 @default.
• W2003137060 hasConcept C19317047 @default.
• W2003137060 hasConcept C202751555 @default.
• W2003137060 hasConcept C203014093 @default.
• W2003137060 hasConcept C2776090121 @default.
• W2003137060 hasConcept C2780007613 @default.
• W2003137060 hasConcept C54355233 @default.
• W2003137060 hasConcept C86803240 @default.
• W2003137060 hasConcept C8891405 @default.
• W2003137060 hasConcept C95444343 @default.
• W2003137060 hasConceptScore W2003137060C104317684 @default.
• W2003137060 hasConceptScore W2003137060C113646796 @default.
• W2003137060 hasConceptScore W2003137060C147969180 @default.
• W2003137060 hasConceptScore W2003137060C153911025 @default.
• W2003137060 hasConceptScore W2003137060C154317977 @default.
• W2003137060 hasConceptScore W2003137060C156695909 @default.
• W2003137060 hasConceptScore W2003137060C167672396 @default.
• W2003137060 hasConceptScore W2003137060C19317047 @default.
• W2003137060 hasConceptScore W2003137060C202751555 @default.
• W2003137060 hasConceptScore W2003137060C203014093 @default.
• W2003137060 hasConceptScore W2003137060C2776090121 @default.
• W2003137060 hasConceptScore W2003137060C2780007613 @default.
• W2003137060 hasConceptScore W2003137060C54355233 @default.
• W2003137060 hasConceptScore W2003137060C86803240 @default.
• W2003137060 hasConceptScore W2003137060C8891405 @default.
• W2003137060 hasConceptScore W2003137060C95444343 @default.
• W2003137060 hasIssue "15" @default.
• W2003137060 hasLocation W20031370601 @default.
• W2003137060 hasLocation W20031370602 @default.
• W2003137060 hasOpenAccess W2003137060 @default.
• W2003137060 hasPrimaryLocation W20031370601 @default.
• W2003137060 hasRelatedWork W1513178816 @default.
• W2003137060 hasRelatedWork W1808357240 @default.
• W2003137060 hasRelatedWork W1976062079 @default.
• W2003137060 hasRelatedWork W2018916009 @default.
• W2003137060 hasRelatedWork W2033962007 @default.
• W2003137060 hasRelatedWork W2050790509 @default.
• W2003137060 hasRelatedWork W2054101033 @default.
• W2003137060 hasRelatedWork W2057419972 @default.
• W2003137060 hasRelatedWork W2125066454 @default.
• W2003137060 hasRelatedWork W2153028546 @default.
• W2003137060 hasVolume "9" @default.
• W2003137060 isParatext "false" @default.

• next