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• W2003142196 abstract "Insect cells were exploited to produce bacterial beta-galactosidase by infecting them with a recombinant nuclear polyhedrosis virus (baculovirus) of Autographa californica. The insect cells were cultured in a continuous stirred tank reactor (CSTR) and led to a second CSTR where they were infected with a recombinant virus in which the lacZ gene from Escherichia coli was inserted. In the effluent of the production reactor, maximum activities of 15 units beta-galactosidase per 10(6) cells were measured. For about 25 d beta-galactosidase production remained constant, but then rapidly declined. This drop was due to a decrease in production of active beta-galactosidase rather than to inactivation of this enzyme. It was concluded that the reduced production was due to reduced polyhedrin promoter-driven synthesis." @default.
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• W2003142196 date "1992-02-01" @default.
• W2003142196 modified "2023-09-27" @default.
• W2003142196 title "Continuous β-galactosidase production with a recombinant baculovirus insect-cell system in bioreactors" @default.
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• W2003142196 doi "https://doi.org/10.1016/0168-1656(92)90147-2" @default.
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