Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003161092> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2003161092 abstract "Large-scale analysis of proteins, which can be regarded as functional biomolecule, assumes an important role in the life science. A MALDI using an ultraviolet laser (UV-MALDI) is one of ionization methods without fragmentation and has achieved conformation analysis of proteins. Recently, protein analysis has shifted from conformation analysis to functional and direct one that reserves posttranslational modifications such as the sugar chain addition and phosphorylation. We have proposed a MALDI using a mid-infrared tunable laser (IR-MALDI) as a new ionization method. IR-MALDI is promising because most biomolecules have a specific absorption in mid-infrared range, and IR-MALDI is expected to offer; (1) use of various matrices, (2) use of biomolecules such as water and lipid as the matrix, and (3) super-soft ionization. First, we evaluated the wavelength dependence of ionization of different matrices using a difference frequency generation (DFG) laser, which can tune the wavelength within a range from 5.5 to 10.0 &mgr;m. As results, ionization was specifically occurred at 5.8 &mgr;m which the C=O vibration stretching bond in matrix material and mass spectrum was observed. Next, protein mass spectrum was observed in the culture cells, MIN6, which secrete insulin, without the conventional cell-preparation processes. We demonstrate that the IR-MALDI has an advantage over the conventional method (UV-MALDI) in direct analysis of intracellular proteins." @default.
- W2003161092 created "2016-06-24" @default.
- W2003161092 creator A5001762466 @default.
- W2003161092 creator A5030105420 @default.
- W2003161092 date "2007-07-05" @default.
- W2003161092 modified "2023-09-23" @default.
- W2003161092 title "Intracellular protein mass spectroscopy using mid-infrared laser ionization" @default.
- W2003161092 doi "https://doi.org/10.1117/12.727753" @default.
- W2003161092 hasPublicationYear "2007" @default.
- W2003161092 type Work @default.
- W2003161092 sameAs 2003161092 @default.
- W2003161092 citedByCount "0" @default.
- W2003161092 crossrefType "proceedings-article" @default.
- W2003161092 hasAuthorship W2003161092A5001762466 @default.
- W2003161092 hasAuthorship W2003161092A5030105420 @default.
- W2003161092 hasConcept C113196181 @default.
- W2003161092 hasConcept C120665830 @default.
- W2003161092 hasConcept C121332964 @default.
- W2003161092 hasConcept C133033976 @default.
- W2003161092 hasConcept C145148216 @default.
- W2003161092 hasConcept C147789679 @default.
- W2003161092 hasConcept C150394285 @default.
- W2003161092 hasConcept C153642686 @default.
- W2003161092 hasConcept C158355884 @default.
- W2003161092 hasConcept C162356407 @default.
- W2003161092 hasConcept C162711632 @default.
- W2003161092 hasConcept C178790620 @default.
- W2003161092 hasConcept C185592680 @default.
- W2003161092 hasConcept C18903297 @default.
- W2003161092 hasConcept C191015642 @default.
- W2003161092 hasConcept C192562407 @default.
- W2003161092 hasConcept C198291218 @default.
- W2003161092 hasConcept C43617362 @default.
- W2003161092 hasConcept C49853544 @default.
- W2003161092 hasConcept C520434653 @default.
- W2003161092 hasConcept C55493867 @default.
- W2003161092 hasConcept C75280812 @default.
- W2003161092 hasConcept C86803240 @default.
- W2003161092 hasConceptScore W2003161092C113196181 @default.
- W2003161092 hasConceptScore W2003161092C120665830 @default.
- W2003161092 hasConceptScore W2003161092C121332964 @default.
- W2003161092 hasConceptScore W2003161092C133033976 @default.
- W2003161092 hasConceptScore W2003161092C145148216 @default.
- W2003161092 hasConceptScore W2003161092C147789679 @default.
- W2003161092 hasConceptScore W2003161092C150394285 @default.
- W2003161092 hasConceptScore W2003161092C153642686 @default.
- W2003161092 hasConceptScore W2003161092C158355884 @default.
- W2003161092 hasConceptScore W2003161092C162356407 @default.
- W2003161092 hasConceptScore W2003161092C162711632 @default.
- W2003161092 hasConceptScore W2003161092C178790620 @default.
- W2003161092 hasConceptScore W2003161092C185592680 @default.
- W2003161092 hasConceptScore W2003161092C18903297 @default.
- W2003161092 hasConceptScore W2003161092C191015642 @default.
- W2003161092 hasConceptScore W2003161092C192562407 @default.
- W2003161092 hasConceptScore W2003161092C198291218 @default.
- W2003161092 hasConceptScore W2003161092C43617362 @default.
- W2003161092 hasConceptScore W2003161092C49853544 @default.
- W2003161092 hasConceptScore W2003161092C520434653 @default.
- W2003161092 hasConceptScore W2003161092C55493867 @default.
- W2003161092 hasConceptScore W2003161092C75280812 @default.
- W2003161092 hasConceptScore W2003161092C86803240 @default.
- W2003161092 hasLocation W20031610921 @default.
- W2003161092 hasOpenAccess W2003161092 @default.
- W2003161092 hasPrimaryLocation W20031610921 @default.
- W2003161092 hasRelatedWork W1485340381 @default.
- W2003161092 hasRelatedWork W1971563831 @default.
- W2003161092 hasRelatedWork W2003161092 @default.
- W2003161092 hasRelatedWork W2051545406 @default.
- W2003161092 hasRelatedWork W2059420989 @default.
- W2003161092 hasRelatedWork W2096150676 @default.
- W2003161092 hasRelatedWork W2137528709 @default.
- W2003161092 hasRelatedWork W2143740707 @default.
- W2003161092 hasRelatedWork W2152045378 @default.
- W2003161092 hasRelatedWork W2473202220 @default.
- W2003161092 isParatext "false" @default.
- W2003161092 isRetracted "false" @default.
- W2003161092 magId "2003161092" @default.
- W2003161092 workType "article" @default.