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- W2003162521 abstract "Endogenous 1α,25-dihydroxyvitamin D3 [1,25-(OH)2D3] in normal rat skin was identified by using thermal isomerization to convert the metabolite into its pre-isomer, high-performance liquid chromatography (HPLC) and displacement potenty with a chick intestinal cytosol receptor. When the metabolite in normal rat skin was determined by a radioreceptor assay after purification by Sep-Pak silica cartridge column chromatography and HPLC, the concentration was 71.0±6.6 pg/g of wet tissue (mean±S.D.). It is also shown that [3H]-1,25-(OH)2D3 and [3H]-25-hydroxyvitamin D3 administered intravenously in the mouse are located in the skin. These results suggest that the metabolite may play an important role in the skin." @default.
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- W2003162521 date "1989-01-01" @default.
- W2003162521 modified "2023-10-18" @default.
- W2003162521 title "Identification and determination of 1α,25-dihydroxyvitamin D3 in rat skin by high-performance liquid chromatography and radioreceptor assay" @default.
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- W2003162521 doi "https://doi.org/10.1016/s0378-4347(00)82709-7" @default.
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