FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003163313> ?p ?o ?g. }

• W2003163313 endingPage "80" @default.
• W2003163313 startingPage "71" @default.
• W2003163313 abstract "A DNA microarray chip of four virulence genes and 16S ribosomal DNA gene conserved region among all Gram negative species, including Yersinia, as a positive control was developed and evaluated using 22 Yersinia enterocolitica isolates. Eight different oligonucleotide probes (oligoprobes) with an average size of 22 bp, complementary to the unique sequences of each gene, were designed and immobilized on the surface of chemically modified slides. Multiplex PCR was used to simultaneously amplify DNA target regions of all five genes, and single stranded DNA (ssDNA) samples for microarray analysis were prepared by using a primer extension of amplicons in the presence of one primer of all genes. The presence of genes in Y. enterocolitica was established by hybridization of the fluorescently labeled ssDNA representing different samples of the microarray gene-specific oligoprobes and confirmed by PCR. Results of the study showed specificity of genotyping Y. enterocolitica using multiple microarray-based assays. Final validation of the chip's ability to identify Y. enterocolitica genes from adulterated pasteurized whole milk was confirmed and successful. The limit of chip detection of virulence genes in pasteurized whole milk was found to be 1000 CFU per hybridization." @default.
• W2003163313 created "2016-06-24" @default.
• W2003163313 creator A5015540601 @default.
• W2003163313 creator A5035537342 @default.
• W2003163313 creator A5072111012 @default.
• W2003163313 date "2006-04-01" @default.
• W2003163313 modified "2023-09-26" @default.
• W2003163313 title "Molecular identification of Yersinia enterocolitica isolated from pasteurized whole milk using DNA microarray chip hybridization" @default.
• W2003163313 cites W1122040095 @default.
• W2003163313 cites W1678792045 @default.
• W2003163313 cites W1995472081 @default.
• W2003163313 cites W2012200656 @default.
• W2003163313 cites W2037360165 @default.
• W2003163313 cites W2050324424 @default.
• W2003163313 cites W2054372867 @default.
• W2003163313 cites W2076765994 @default.
• W2003163313 cites W2102847011 @default.
• W2003163313 cites W2107304140 @default.
• W2003163313 cites W2108957388 @default.
• W2003163313 cites W2113883532 @default.
• W2003163313 cites W2114226204 @default.
• W2003163313 cites W2122096837 @default.
• W2003163313 cites W2122809186 @default.
• W2003163313 cites W2126361348 @default.
• W2003163313 cites W2128367620 @default.
• W2003163313 cites W2129135399 @default.
• W2003163313 cites W2133247102 @default.
• W2003163313 cites W2137621403 @default.
• W2003163313 cites W2143881572 @default.
• W2003163313 cites W2146254807 @default.
• W2003163313 cites W2155203141 @default.
• W2003163313 cites W2155329697 @default.
• W2003163313 cites W2164147412 @default.
• W2003163313 cites W2169898067 @default.
• W2003163313 cites W29042828 @default.
• W2003163313 doi "https://doi.org/10.1016/j.mcp.2005.09.006" @default.
• W2003163313 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16330182" @default.
• W2003163313 hasPublicationYear "2006" @default.
• W2003163313 type Work @default.
• W2003163313 sameAs 2003163313 @default.
• W2003163313 citedByCount "40" @default.
• W2003163313 countsByYear W20031633132012 @default.
• W2003163313 countsByYear W20031633132013 @default.
• W2003163313 countsByYear W20031633132014 @default.
• W2003163313 countsByYear W20031633132015 @default.
• W2003163313 countsByYear W20031633132016 @default.
• W2003163313 countsByYear W20031633132017 @default.
• W2003163313 countsByYear W20031633132018 @default.
• W2003163313 countsByYear W20031633132019 @default.
• W2003163313 countsByYear W20031633132020 @default.
• W2003163313 countsByYear W20031633132021 @default.
• W2003163313 crossrefType "journal-article" @default.
• W2003163313 hasAuthorship W2003163313A5015540601 @default.
• W2003163313 hasAuthorship W2003163313A5035537342 @default.
• W2003163313 hasAuthorship W2003163313A5072111012 @default.
• W2003163313 hasConcept C104317684 @default.
• W2003163313 hasConcept C129312508 @default.
• W2003163313 hasConcept C130197536 @default.
• W2003163313 hasConcept C135763542 @default.
• W2003163313 hasConcept C150194340 @default.
• W2003163313 hasConcept C153911025 @default.
• W2003163313 hasConcept C178790620 @default.
• W2003163313 hasConcept C185592680 @default.
• W2003163313 hasConcept C186836561 @default.
• W2003163313 hasConcept C2776234502 @default.
• W2003163313 hasConcept C2777563447 @default.
• W2003163313 hasConcept C31467283 @default.
• W2003163313 hasConcept C49105822 @default.
• W2003163313 hasConcept C523546767 @default.
• W2003163313 hasConcept C54355233 @default.
• W2003163313 hasConcept C552990157 @default.
• W2003163313 hasConcept C60987743 @default.
• W2003163313 hasConcept C8185291 @default.
• W2003163313 hasConcept C86803240 @default.
• W2003163313 hasConcept C90583042 @default.
• W2003163313 hasConcept C95371953 @default.
• W2003163313 hasConceptScore W2003163313C104317684 @default.
• W2003163313 hasConceptScore W2003163313C129312508 @default.
• W2003163313 hasConceptScore W2003163313C130197536 @default.
• W2003163313 hasConceptScore W2003163313C135763542 @default.
• W2003163313 hasConceptScore W2003163313C150194340 @default.
• W2003163313 hasConceptScore W2003163313C153911025 @default.
• W2003163313 hasConceptScore W2003163313C178790620 @default.
• W2003163313 hasConceptScore W2003163313C185592680 @default.
• W2003163313 hasConceptScore W2003163313C186836561 @default.
• W2003163313 hasConceptScore W2003163313C2776234502 @default.
• W2003163313 hasConceptScore W2003163313C2777563447 @default.
• W2003163313 hasConceptScore W2003163313C31467283 @default.
• W2003163313 hasConceptScore W2003163313C49105822 @default.
• W2003163313 hasConceptScore W2003163313C523546767 @default.
• W2003163313 hasConceptScore W2003163313C54355233 @default.
• W2003163313 hasConceptScore W2003163313C552990157 @default.
• W2003163313 hasConceptScore W2003163313C60987743 @default.
• W2003163313 hasConceptScore W2003163313C8185291 @default.
• W2003163313 hasConceptScore W2003163313C86803240 @default.
• W2003163313 hasConceptScore W2003163313C90583042 @default.
• W2003163313 hasConceptScore W2003163313C95371953 @default.
• W2003163313 hasIssue "2" @default.

• next