FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003167114> ?p ?o ?g. }

• W2003167114 endingPage "S782" @default.
• W2003167114 startingPage "S782" @default.
• W2003167114 abstract "The radiobiology of induced pluripotent stem cells (iPSC) compared to fibroblast progenitors has not been reported. An iPSC cell line was derived from a single individual's fibroblasts (FB) by transfection with 4 genes (OCT4, SOX2, NONOG and Lin28). We hypothesized that iPSCs would be radiosensitive relative to parental FB cells by reduced viability, increased apoptosis, depleted antioxidant stores and greater cell cycle arrest following irradiation. iPSCs and FB cells were irradiated to doses of 0, 0.5, 1.0, 1.5, 2.0 or 2.5 Gy at 600 mu/min using 6 MV photons. Viability was measured over 24 hr by trypan blue exclusion, percent of apoptotic cells by Annexin V and flow cytometer, cell cycle analysis by fixing cells with ice cold 70% ethanol for 24 hr, staining with propidium iodide and analyzing cells by Accun C-6 flow cytometer, and antioxidant stores by trolox-units in a commercial kit. Viability of iPSCs decreased significantly 24 hr following irradiation: 75.3 ± 3.9% at 0 Gy, 67.2 ± 2.4% after 0.5 Gy, 51.0 ± 6.1% following 1.0 Gy (p = 0.0010 compared to 0 Gy), 41.8 ± 3.3 after 1.5 Gy (p = 0.0010), 32.4 ± 5.2% after 2.0 Gy (p = 0.0009), and 32.3 ± 6.6% following 2.5 Gy (p = 0.0020). In contrast FB cells showed no viability loss after the same irradiation doses: 98.6 ± 0.3% at 0 Gy and 98.3 ± 0.4% following 2.5 Gy. iPSCs cells displayed significantly greater apoptosis: 13.6 ± 1.8% after 0 Gy, 16.1 ± 2.8% following 0.5 Gy, 33.5 ± 5.8% after 1.0 Gy (p = 0.0172 compared to 0 Gy), 35.7 ± 3.0% after 1.5 Gy (p = 0.0008), 50.3 ± 4.3% after 2.0 Gy (p = 0.0002) and 41.6 ± 6.9% following 2.5 Gy (p = 0.0078). In contrast, FB cells showed no significant apoptosis: 0 Gy, 0.6 ± 0.4% apoptotic cells, 0.7 ± 0.1% following 2.5 Gy. Antioxidant stores were higher in FB cells before irradiation compared to iPSC (0.051 ± 0.011, and 0.027 ± 0.002 trolox equivalents, respectively, p = 0.0197). However, one hour after 1 Gy, iPSC had higher levels of antioxidants than did FB cells (0.127 ± 0.001 and 0.043 ± 0.003, respectively, p < 0.0001). By 48 hrs FB antioxidant stores were greater than those in iPSCs (0.079 ± 0.011 and 0.0229 ± 0.013, respectively, p = 0.0319). There were no differences in cell cycle distribution in either cell phenotype after these irradiation doses. Human iPSCs are radiosensitive to doses of 0.5-2.5 Gy compared to their parental fibroblasts and may be a valuable platform for analysis of the radiobiologic effects of each of the transgenes inducing the iPSC phenotype." @default.
• W2003167114 created "2016-06-24" @default.
• W2003167114 creator A5016542008 @default.
• W2003167114 creator A5018623684 @default.
• W2003167114 creator A5055843706 @default.
• W2003167114 creator A5063266916 @default.
• W2003167114 creator A5064352152 @default.
• W2003167114 creator A5072619626 @default.
• W2003167114 creator A5078588576 @default.
• W2003167114 creator A5080313123 @default.
• W2003167114 creator A5088260835 @default.
• W2003167114 date "2014-09-01" @default.
• W2003167114 modified "2023-09-30" @default.
• W2003167114 title "Radiosensitivity of Human Induced Pluripotent Stem Cells Compared to Parental Fibroblasts" @default.
• W2003167114 doi "https://doi.org/10.1016/j.ijrobp.2014.05.2262" @default.
• W2003167114 hasPublicationYear "2014" @default.
• W2003167114 type Work @default.
• W2003167114 sameAs 2003167114 @default.
• W2003167114 citedByCount "0" @default.
• W2003167114 crossrefType "journal-article" @default.
• W2003167114 hasAuthorship W2003167114A5016542008 @default.
• W2003167114 hasAuthorship W2003167114A5018623684 @default.
• W2003167114 hasAuthorship W2003167114A5055843706 @default.
• W2003167114 hasAuthorship W2003167114A5063266916 @default.
• W2003167114 hasAuthorship W2003167114A5064352152 @default.
• W2003167114 hasAuthorship W2003167114A5072619626 @default.
• W2003167114 hasAuthorship W2003167114A5078588576 @default.
• W2003167114 hasAuthorship W2003167114A5080313123 @default.
• W2003167114 hasAuthorship W2003167114A5088260835 @default.
• W2003167114 hasBestOaLocation W20031671141 @default.
• W2003167114 hasConcept C104317684 @default.
• W2003167114 hasConcept C107459253 @default.
• W2003167114 hasConcept C126322002 @default.
• W2003167114 hasConcept C145103041 @default.
• W2003167114 hasConcept C153911025 @default.
• W2003167114 hasConcept C16685009 @default.
• W2003167114 hasConcept C190283241 @default.
• W2003167114 hasConcept C206684579 @default.
• W2003167114 hasConcept C2775934118 @default.
• W2003167114 hasConcept C2780070996 @default.
• W2003167114 hasConcept C31573885 @default.
• W2003167114 hasConcept C509974204 @default.
• W2003167114 hasConcept C53227056 @default.
• W2003167114 hasConcept C55493867 @default.
• W2003167114 hasConcept C71924100 @default.
• W2003167114 hasConcept C86803240 @default.
• W2003167114 hasConceptScore W2003167114C104317684 @default.
• W2003167114 hasConceptScore W2003167114C107459253 @default.
• W2003167114 hasConceptScore W2003167114C126322002 @default.
• W2003167114 hasConceptScore W2003167114C145103041 @default.
• W2003167114 hasConceptScore W2003167114C153911025 @default.
• W2003167114 hasConceptScore W2003167114C16685009 @default.
• W2003167114 hasConceptScore W2003167114C190283241 @default.
• W2003167114 hasConceptScore W2003167114C206684579 @default.
• W2003167114 hasConceptScore W2003167114C2775934118 @default.
• W2003167114 hasConceptScore W2003167114C2780070996 @default.
• W2003167114 hasConceptScore W2003167114C31573885 @default.
• W2003167114 hasConceptScore W2003167114C509974204 @default.
• W2003167114 hasConceptScore W2003167114C53227056 @default.
• W2003167114 hasConceptScore W2003167114C55493867 @default.
• W2003167114 hasConceptScore W2003167114C71924100 @default.
• W2003167114 hasConceptScore W2003167114C86803240 @default.
• W2003167114 hasIssue "1" @default.
• W2003167114 hasLocation W20031671141 @default.
• W2003167114 hasOpenAccess W2003167114 @default.
• W2003167114 hasPrimaryLocation W20031671141 @default.
• W2003167114 hasRelatedWork W1558034455 @default.
• W2003167114 hasRelatedWork W197592292 @default.
• W2003167114 hasRelatedWork W1991369368 @default.
• W2003167114 hasRelatedWork W2057959589 @default.
• W2003167114 hasRelatedWork W2151975024 @default.
• W2003167114 hasRelatedWork W2158060338 @default.
• W2003167114 hasRelatedWork W2220797277 @default.
• W2003167114 hasRelatedWork W2337084509 @default.
• W2003167114 hasRelatedWork W2396265220 @default.
• W2003167114 hasRelatedWork W3165528361 @default.
• W2003167114 hasVolume "90" @default.
• W2003167114 isParatext "false" @default.
• W2003167114 isRetracted "false" @default.
• W2003167114 magId "2003167114" @default.
• W2003167114 workType "article" @default.