FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003173831> ?p ?o ?g. }

• W2003173831 endingPage "e64139" @default.
• W2003173831 startingPage "e64139" @default.
• W2003173831 abstract "UROtsa is an authentic, immortalized human urothelial cell line that is used to study the effects of metals and other toxic substances, mostly in the context of bladder cancer carcinogenesis. Unusual properties on the molecular level of a provided UROtsa cell line stock prompted us to verify its identity.UROtsa cell line stocks from different sources were tested on several molecular levels and compared with other cell lines. MicroRNA and mRNA expression was determined by Real-Time PCR. Chromosome numbers were checked and PCR of different regions of the large T-antigen was performed. DNA methylation of RARB, PGR, RASSF1, CDH1, FHIT, ESR1, C1QTNF6, PTGS2, SOCS3, MGMT, and LINE1 was analyzed by pyrosequencing and compared with results from the cell lines RT4, T24, HeLa, BEAS-2B, and HepG2. Finally, short tandem repeat (STR) profiling was applied.All tested UROtsa cell line stocks lacked large T-antigen. STR analysis unequivocally identified our main UROtsa stock as the bladder cancer cell line T24, which was different from two authentic UROtsa stocks that served as controls. Analysis of DNA methylation patterns and RNA expression confirmed their differences. Methylation pattern and mRNA expression of the contaminating T24 cell line showed moderate changes even after long-term culture of up to 56 weeks, whereas miRNAs and chromosome numbers varied markedly.It is important to check the identity of cell lines, especially those that are not distributed by major cell banks. However, for some cell lines STR profiles are not available. Therefore, new cell lines should either be submitted to cell banks or at least their STR profile determined and published as part of their initial characterization. Our results should help to improve the identification of UROtsa and other cells on different molecular levels and provide information on the use of urothelial cells for long-term experiments." @default.
• W2003173831 created "2016-06-24" @default.
• W2003173831 creator A5012642399 @default.
• W2003173831 creator A5020251237 @default.
• W2003173831 creator A5023144933 @default.
• W2003173831 creator A5029380251 @default.
• W2003173831 creator A5038994918 @default.
• W2003173831 creator A5043756488 @default.
• W2003173831 creator A5047519172 @default.
• W2003173831 creator A5048814965 @default.
• W2003173831 creator A5059239176 @default.
• W2003173831 creator A5070626446 @default.
• W2003173831 creator A5075945234 @default.
• W2003173831 creator A5089668685 @default.
• W2003173831 date "2013-05-17" @default.
• W2003173831 modified "2023-09-26" @default.
• W2003173831 title "Cross-Contamination of a UROtsa Stock with T24 Cells – Molecular Comparison of Different Cell Lines and Stocks" @default.
• W2003173831 cites W142827737 @default.
• W2003173831 cites W1741126710 @default.
• W2003173831 cites W1869191601 @default.
• W2003173831 cites W1964443736 @default.
• W2003173831 cites W1964639350 @default.
• W2003173831 cites W1971478519 @default.
• W2003173831 cites W1972504105 @default.
• W2003173831 cites W1972944037 @default.
• W2003173831 cites W1976265002 @default.
• W2003173831 cites W1980890490 @default.
• W2003173831 cites W1982328256 @default.
• W2003173831 cites W1986895526 @default.
• W2003173831 cites W1987285533 @default.
• W2003173831 cites W1990148217 @default.
• W2003173831 cites W1996053208 @default.
• W2003173831 cites W2000248995 @default.
• W2003173831 cites W2002195470 @default.
• W2003173831 cites W2002880821 @default.
• W2003173831 cites W2009857031 @default.
• W2003173831 cites W2013624745 @default.
• W2003173831 cites W2016085865 @default.
• W2003173831 cites W2025392244 @default.
• W2003173831 cites W2029209435 @default.
• W2003173831 cites W2036801235 @default.
• W2003173831 cites W2036986058 @default.
• W2003173831 cites W2038099835 @default.
• W2003173831 cites W2038978121 @default.
• W2003173831 cites W2039528775 @default.
• W2003173831 cites W2039599418 @default.
• W2003173831 cites W2042968462 @default.
• W2003173831 cites W2045970082 @default.
• W2003173831 cites W2049658049 @default.
• W2003173831 cites W2050632329 @default.
• W2003173831 cites W2058218706 @default.
• W2003173831 cites W2061994273 @default.
• W2003173831 cites W2062085206 @default.
• W2003173831 cites W2063986226 @default.
• W2003173831 cites W2073353870 @default.
• W2003173831 cites W2074929084 @default.
• W2003173831 cites W2083603631 @default.
• W2003173831 cites W2086201153 @default.
• W2003173831 cites W2087638253 @default.
• W2003173831 cites W2089001033 @default.
• W2003173831 cites W2091273862 @default.
• W2003173831 cites W2097005928 @default.
• W2003173831 cites W2097275308 @default.
• W2003173831 cites W2100475900 @default.
• W2003173831 cites W2106958499 @default.
• W2003173831 cites W2107832763 @default.
• W2003173831 cites W2113001012 @default.
• W2003173831 cites W2127616861 @default.
• W2003173831 cites W2130967251 @default.
• W2003173831 cites W2137519140 @default.
• W2003173831 cites W2137915907 @default.
• W2003173831 cites W2139559241 @default.
• W2003173831 cites W2141764094 @default.
• W2003173831 cites W2145757364 @default.
• W2003173831 cites W2150507760 @default.
• W2003173831 cites W2154891376 @default.
• W2003173831 cites W2155372650 @default.
• W2003173831 cites W2157731375 @default.
• W2003173831 cites W2170650990 @default.
• W2003173831 cites W2170844556 @default.
• W2003173831 cites W2178005554 @default.
• W2003173831 cites W2259938310 @default.
• W2003173831 cites W4361804794 @default.
• W2003173831 cites W3149786887 @default.
• W2003173831 doi "https://doi.org/10.1371/journal.pone.0064139" @default.
• W2003173831 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3656924" @default.
• W2003173831 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23691160" @default.
• W2003173831 hasPublicationYear "2013" @default.
• W2003173831 type Work @default.
• W2003173831 sameAs 2003173831 @default.
• W2003173831 citedByCount "16" @default.
• W2003173831 countsByYear W20031738312013 @default.
• W2003173831 countsByYear W20031738312014 @default.
• W2003173831 countsByYear W20031738312015 @default.
• W2003173831 countsByYear W20031738312016 @default.
• W2003173831 countsByYear W20031738312017 @default.
• W2003173831 countsByYear W20031738312018 @default.
• W2003173831 countsByYear W20031738312021 @default.

• next