Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003175590> ?p ?o ?g. }
- W2003175590 endingPage "175" @default.
- W2003175590 startingPage "166" @default.
- W2003175590 abstract "There is an increasing need to develop improved systems for predicting the safety of xenobiotics. However, to move beyond hazard identification the available concentration of the test compounds needs to be incorporated. In this study cyclosporine A (CsA) was used as a model compound to assess the kinetic profiles in two rodent brain cell cultures after single and repeated exposures. CsA induced-cyclophilin B (Cyp-B) secretion was also determined as CsA-specific pharmacodynamic endpoint. Since CsA is a potent p-glycoprotein substrate, the ability of this compound to cross the blood–brain barrier (BBB) was also investigated using an in vitro bovine model with repeated exposures up to 14 days. Finally, CsA uptake mechanisms were studied using a parallel artificial membrane assay (PAMPA) in combination with a Caco-2 model. Kinetic results indicate a low intracellular CsA uptake, with no marked bioaccumulation or biotransformation. In addition, only low CsA amounts crossed the BBB. PAMPA and Caco-2 experiments revealed that CsA is mostly trapped to lipophilic compartments and exits the cell apically via active transport. Thus, although CsA is unlikely to enter the brain at cytotoxic concentrations, it may cause alterations in electrical activity and is likely to increase the CNS concentration of other compounds by occupying the BBBs extrusion capacity. Such an integrated testing system, incorporating BBB, brain culture models and kinetics could be applied for assessing neurotoxicity potential of compounds." @default.
- W2003175590 created "2016-06-24" @default.
- W2003175590 creator A5009000656 @default.
- W2003175590 creator A5016801110 @default.
- W2003175590 creator A5021308779 @default.
- W2003175590 creator A5021813622 @default.
- W2003175590 creator A5022092991 @default.
- W2003175590 creator A5023447475 @default.
- W2003175590 creator A5028987042 @default.
- W2003175590 creator A5031256421 @default.
- W2003175590 creator A5040695300 @default.
- W2003175590 creator A5044461786 @default.
- W2003175590 creator A5048155890 @default.
- W2003175590 creator A5058157647 @default.
- W2003175590 creator A5088509681 @default.
- W2003175590 date "2015-12-01" @default.
- W2003175590 modified "2023-10-18" @default.
- W2003175590 title "Cyclosporine A kinetics in brain cell cultures and its potential of crossing the blood–brain barrier" @default.
- W2003175590 cites W1558311166 @default.
- W2003175590 cites W1588361932 @default.
- W2003175590 cites W1872613740 @default.
- W2003175590 cites W1890478772 @default.
- W2003175590 cites W1923525646 @default.
- W2003175590 cites W1968779484 @default.
- W2003175590 cites W1972344503 @default.
- W2003175590 cites W1977540882 @default.
- W2003175590 cites W1979409628 @default.
- W2003175590 cites W1979770296 @default.
- W2003175590 cites W1988499440 @default.
- W2003175590 cites W1989347679 @default.
- W2003175590 cites W1997211805 @default.
- W2003175590 cites W1997866449 @default.
- W2003175590 cites W2004783028 @default.
- W2003175590 cites W2007541245 @default.
- W2003175590 cites W2007880547 @default.
- W2003175590 cites W2015757448 @default.
- W2003175590 cites W2017411996 @default.
- W2003175590 cites W2018626384 @default.
- W2003175590 cites W2022072609 @default.
- W2003175590 cites W2028303959 @default.
- W2003175590 cites W2032779863 @default.
- W2003175590 cites W2032865438 @default.
- W2003175590 cites W2034999905 @default.
- W2003175590 cites W2038054558 @default.
- W2003175590 cites W2041798348 @default.
- W2003175590 cites W2042021237 @default.
- W2003175590 cites W2042096520 @default.
- W2003175590 cites W2042436328 @default.
- W2003175590 cites W2045312970 @default.
- W2003175590 cites W2049439993 @default.
- W2003175590 cites W2049520769 @default.
- W2003175590 cites W2052907531 @default.
- W2003175590 cites W2056188359 @default.
- W2003175590 cites W2059076872 @default.
- W2003175590 cites W2065812641 @default.
- W2003175590 cites W2065892759 @default.
- W2003175590 cites W2066609845 @default.
- W2003175590 cites W2067111489 @default.
- W2003175590 cites W2069335698 @default.
- W2003175590 cites W2069388748 @default.
- W2003175590 cites W2082043748 @default.
- W2003175590 cites W2083673453 @default.
- W2003175590 cites W2084148692 @default.
- W2003175590 cites W2087508160 @default.
- W2003175590 cites W2091509247 @default.
- W2003175590 cites W2096503469 @default.
- W2003175590 cites W2111001050 @default.
- W2003175590 cites W2113656727 @default.
- W2003175590 cites W2116882304 @default.
- W2003175590 cites W2118745556 @default.
- W2003175590 cites W2133475198 @default.
- W2003175590 cites W2145975509 @default.
- W2003175590 cites W2154008536 @default.
- W2003175590 cites W2154406308 @default.
- W2003175590 cites W2160917955 @default.
- W2003175590 cites W2165821159 @default.
- W2003175590 cites W2170271130 @default.
- W2003175590 cites W2201097810 @default.
- W2003175590 cites W2409359475 @default.
- W2003175590 cites W4233053008 @default.
- W2003175590 cites W4248107770 @default.
- W2003175590 cites W66351836 @default.
- W2003175590 cites W876388741 @default.
- W2003175590 doi "https://doi.org/10.1016/j.tiv.2015.01.003" @default.
- W2003175590 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25683621" @default.
- W2003175590 hasPublicationYear "2015" @default.
- W2003175590 type Work @default.
- W2003175590 sameAs 2003175590 @default.
- W2003175590 citedByCount "21" @default.
- W2003175590 countsByYear W20031755902015 @default.
- W2003175590 countsByYear W20031755902017 @default.
- W2003175590 countsByYear W20031755902018 @default.
- W2003175590 countsByYear W20031755902019 @default.
- W2003175590 countsByYear W20031755902020 @default.
- W2003175590 countsByYear W20031755902021 @default.
- W2003175590 countsByYear W20031755902022 @default.
- W2003175590 countsByYear W20031755902023 @default.
- W2003175590 crossrefType "journal-article" @default.