FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003176139> ?p ?o ?g. }

• W2003176139 abstract "Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, ILProgression of tumor size greater than 2-5mm in size requires induction of angiogenesis to supply the tumor with oxygen and nutrients. Angiogenesis occurs due to intra-tumoral cell release of endothelial mitogenic factors in response to hypoxia or genetic mutation. There are currently numerous endogenous proteins in clinical development as therapeutic anti-angiogenesis targets e.g. VEGF and PlGF. Herein, we have investigated a novel anti-angiogenic agent API 31510 currently subject of late stage clinical trials in skin cancers and a Phase I for solid tumors. Human umbilical vein endothelial cell (HUVEC) fate decisions that modulate the angiogenic phenotype were examined in the presence of 100 µM or 1500 µM 31510 or excipient and compared to untreated control cells. Endothelial cell fate assays for apoptosis, proliferation, migration and 3-D tube formation within matrigel were performed. Morphological & flow cytometric analysis of annexin V/propidium iodide positive cells revealed an increase in HUVEC apoptosis in the presence of 1500 µM 31510, compared to excipient or control cells. Concomitant with increased cell death due to 31510, HUVEC cell counts were significantly decreased in the presence of 1500 µM 31510. To assess the potential effects of 31510 on endothelial migration, we examined HUVEC migration 5 hours post-wound generation, in an endothelial scratch assay. Both 31510 and excipient significantly impaired HUVEC migration at both 100 and 1500 µM concentration, demonstrating anti-migratory activity of both the excipient and 31510. In order to determine if the 31510 anti-tumor activity is due to effects on endothelial sprouting angiogenesis, we examined endothelial tube formation in 3-D matrigel cultures over time. Addition of 1500 µM excipient in both the gel and overlying media impaired tube formation compared to control. Moreover, addition of 1500 µM 31510 further impaired HUVEC tube formation compared to both excipient and control untreated cells. These effects were noted as early as 24 hours after seeding and up to 96 hours in culture. Moreover, histological analyses from excised tumors from pre-clinical animal models in melanoma, SCC, liver cancer, and pancreatic cancer confirm a hallmark downregulation in angiogenic signaling and infrastructure. Anti-tumor activity of 31510 is likely due, at least is part, to inhibition of tumor recruitment of local blood supply for neo-vessel formation. Taken together, these studies reveal that 31510 alters endothelial migration, proliferation, apoptosis and tube formation. These studies strongly support a role for API 31510 as a novel anti-angiogenic agent that limits the angiogenic capacity of endothelial cells while modulating tumor angiogenesis.Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2335. doi:1538-7445.AM2012-2335" @default.
• W2003176139 created "2016-06-24" @default.
• W2003176139 creator A5024270531 @default.
• W2003176139 creator A5027320426 @default.
• W2003176139 creator A5058081424 @default.
• W2003176139 creator A5074830972 @default.
• W2003176139 creator A5090366304 @default.
• W2003176139 date "2012-04-15" @default.
• W2003176139 modified "2023-09-25" @default.
• W2003176139 title "Abstract 2335: API 31510 - a novel anti-angiogenic modulator of endothelial cells and the tumor microenvironment" @default.
• W2003176139 doi "https://doi.org/10.1158/1538-7445.am2012-2335" @default.
• W2003176139 hasPublicationYear "2012" @default.
• W2003176139 type Work @default.
• W2003176139 sameAs 2003176139 @default.
• W2003176139 citedByCount "0" @default.
• W2003176139 crossrefType "proceedings-article" @default.
• W2003176139 hasAuthorship W2003176139A5024270531 @default.
• W2003176139 hasAuthorship W2003176139A5027320426 @default.
• W2003176139 hasAuthorship W2003176139A5058081424 @default.
• W2003176139 hasAuthorship W2003176139A5074830972 @default.
• W2003176139 hasAuthorship W2003176139A5090366304 @default.
• W2003176139 hasConcept C123012128 @default.
• W2003176139 hasConcept C137738243 @default.
• W2003176139 hasConcept C1491633281 @default.
• W2003176139 hasConcept C190283241 @default.
• W2003176139 hasConcept C202751555 @default.
• W2003176139 hasConcept C203014093 @default.
• W2003176139 hasConcept C2775934118 @default.
• W2003176139 hasConcept C2776107976 @default.
• W2003176139 hasConcept C2777411675 @default.
• W2003176139 hasConcept C2777834122 @default.
• W2003176139 hasConcept C2778608917 @default.
• W2003176139 hasConcept C2780394083 @default.
• W2003176139 hasConcept C3020616263 @default.
• W2003176139 hasConcept C31573885 @default.
• W2003176139 hasConcept C502942594 @default.
• W2003176139 hasConcept C553184892 @default.
• W2003176139 hasConcept C55493867 @default.
• W2003176139 hasConcept C71924100 @default.
• W2003176139 hasConcept C86803240 @default.
• W2003176139 hasConcept C88634738 @default.
• W2003176139 hasConceptScore W2003176139C123012128 @default.
• W2003176139 hasConceptScore W2003176139C137738243 @default.
• W2003176139 hasConceptScore W2003176139C1491633281 @default.
• W2003176139 hasConceptScore W2003176139C190283241 @default.
• W2003176139 hasConceptScore W2003176139C202751555 @default.
• W2003176139 hasConceptScore W2003176139C203014093 @default.
• W2003176139 hasConceptScore W2003176139C2775934118 @default.
• W2003176139 hasConceptScore W2003176139C2776107976 @default.
• W2003176139 hasConceptScore W2003176139C2777411675 @default.
• W2003176139 hasConceptScore W2003176139C2777834122 @default.
• W2003176139 hasConceptScore W2003176139C2778608917 @default.
• W2003176139 hasConceptScore W2003176139C2780394083 @default.
• W2003176139 hasConceptScore W2003176139C3020616263 @default.
• W2003176139 hasConceptScore W2003176139C31573885 @default.
• W2003176139 hasConceptScore W2003176139C502942594 @default.
• W2003176139 hasConceptScore W2003176139C553184892 @default.
• W2003176139 hasConceptScore W2003176139C55493867 @default.
• W2003176139 hasConceptScore W2003176139C71924100 @default.
• W2003176139 hasConceptScore W2003176139C86803240 @default.
• W2003176139 hasConceptScore W2003176139C88634738 @default.
• W2003176139 hasLocation W20031761391 @default.
• W2003176139 hasOpenAccess W2003176139 @default.
• W2003176139 hasPrimaryLocation W20031761391 @default.
• W2003176139 hasRelatedWork W1996467898 @default.
• W2003176139 hasRelatedWork W2006950015 @default.
• W2003176139 hasRelatedWork W2015652242 @default.
• W2003176139 hasRelatedWork W2032680197 @default.
• W2003176139 hasRelatedWork W2036760060 @default.
• W2003176139 hasRelatedWork W2056549984 @default.
• W2003176139 hasRelatedWork W2063454608 @default.
• W2003176139 hasRelatedWork W2065103997 @default.
• W2003176139 hasRelatedWork W2093488145 @default.
• W2003176139 hasRelatedWork W2100361141 @default.
• W2003176139 hasRelatedWork W2130499442 @default.
• W2003176139 hasRelatedWork W2226803679 @default.
• W2003176139 hasRelatedWork W2317804157 @default.
• W2003176139 hasRelatedWork W2334676145 @default.
• W2003176139 hasRelatedWork W2382945073 @default.
• W2003176139 hasRelatedWork W2415365722 @default.
• W2003176139 hasRelatedWork W2530443903 @default.
• W2003176139 hasRelatedWork W2559817919 @default.
• W2003176139 hasRelatedWork W2739617526 @default.
• W2003176139 hasRelatedWork W2893750600 @default.
• W2003176139 isParatext "false" @default.
• W2003176139 isRetracted "false" @default.
• W2003176139 magId "2003176139" @default.
• W2003176139 workType "article" @default.