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- W2003180322 abstract "Abstract We present three generations of microchip‐based “in‐space” sample fractionators and collectors for use in proteomics. The basic chip design consisted of a single channel for CE separation of analytes that then intersects a fractionation zone feed into multiple high aspect ratio microchannels for fractionation of separated components. Achievements of each generation are discussed in relation to important design criteria. CE‐separated samples were electrokinetically driven to multiple collection channels in sequence without cross‐contamination under the protection of sheath streams. A 36‐channel fractionator demonstrated the efficacy of a high‐throughput fractionator with no observed cross‐contamination. A mixture of IgG and BSA was used to test the efficiency of the fractionator and collector. CE of the fractionated samples was performed on the same device to verify their purity. Our demonstration proved to be efficient and reproducible in obtaining non‐contaminated samples over 15 sample injections. Experimental results were found to be in close agreement with PSpice simulation in terms of flow behavior, contamination control and device performance. The design presented here has a great potential to be integrated in proteomic platforms." @default.
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- W2003180322 date "2010-07-23" @default.
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- W2003180322 title "Microfluidic devices for electrokinetic sample fractionation" @default.
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- W2003180322 doi "https://doi.org/10.1002/elps.201000098" @default.
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