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• W2003183224 abstract "The synthesis of a polyhydroxyalkanoate with medium chain length alkyl substituents by Pseudomonas oleovorans was investigated using protonated and deuterated forms of octanoic acid in a minimal salts medium. Cultivation with deuterated octanoic acid resulted in a reduced rate of polymer accumulation compared to that with its protonated counterpart (107 and 207 mg of polymer L-1 of medium h-1 of cultivation, respectively). Nuclear magnetic resonance and gas chromatography coupled mass spectrometry of the derivatized polymer was used to establish the extent and distribution of deuterium in the biopolymer. A partially deuterated heteropolymer with 3-hydroxyoctanoic acid as the main constituent was produced. Deuteration is an important tool for contrast variation studies using neutron scattering, but predicates that the deuterated polymer is otherwise comparable in its physiochemical and material properties to its protonated counterpart. In studies reported here, the deuterated biopolymer exhibited an additional diffraction maximum at 7.55 Å and slight differences in its melting point (60 and 55 °C) and glass transition temperature (−39 and −36 °C) when compared to its protonated equivalent. While significant differences between the protonated and deuterated biopolymers were determined, our results support the use of this deuterated polyhydroxyalkanoate in its application in investigations using analytical neutron scattering techniques." @default.
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• W2003183224 date "2006-02-28" @default.
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• W2003183224 title "Biosynthesis and Characterization of Deuterated Polyhydroxyoctanoate" @default.
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• W2003183224 doi "https://doi.org/10.1021/bm050969l" @default.
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