FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003193484> ?p ?o ?g. }

• W2003193484 endingPage "875" @default.
• W2003193484 startingPage "868" @default.
• W2003193484 abstract "OBJECTIVE To study the involvement of extracellular Ca 2+ and the properties of the intracellular Ca 2+ ([Ca 2+ ] i ) stores on the carbachol‐induced contraction of mammalian urinary bladder smooth muscle strips under polarized and depolarized conditions. MATERIALS AND METHODS Strips of bladder were suspended between platinum ring electrodes in a cylindrical organ bath (0.2 mL) and continuously superfused with Krebs’ solution at 1 mL/min. The effect of nifedipine, cyclopiazonic acid (CPA), thapsigargin, procaine, ryanodine and caffeine before and during a 10‐s application of 100 µ m carbachol under polarized conditions were studied. The effect of these drugs was also assessed under depolarized conditions using a protocol that allowed a more detailed assessment of the role of [Ca 2+ ] i stores, consisting of emptying the stores by exposure to Ca 2+ ‐free solution, rapidly refilling them by a 10‐s application of 81.5 m m Ca 2+ (priming), returning to the Ca 2+ ‐free solution for 3 min and then applying 100 µ m carbachol (10 s) in Ca 2+ ‐free solution (store release). RESULTS Under polarized conditions, nifedipine and Ca 2+ removal almost completely inhibited the carbachol‐induced contractions. CPA increased the amplitude and duration of both carbachol‐ and electrical field stimulation‐induced contractions. Although ryanodine had no inhibitory effect, caffeine and procaine significantly inhibited the carbachol‐induced contraction. Under depolarized conditions nifedipine blocked both priming and store release contractions. CPA, thapsigargin, procaine and ryanodine significantly increased the priming and inhibited the store release contractions. However, caffeine virtually abolished both priming and store release contractions. CONCLUSION These results suggest that in guinea‐pig urinary bladder smooth muscle the Ca 2+ necessary for contraction enters the cell through voltage‐dependent dihydropiridine‐sensitive Ca 2+ channels and is pumped into an intracellular store that is released by carbachol. Under polarized conditions, the blockade of sarco‐endoplasmic reticulum calcium ATP‐ase (SERCA) with CPA increases [Ca 2+ ] i and carbachol‐induced contractions. The effects of caffeine and procaine suggest that store release involves ryanodine receptors and calcium‐induced calcium release. Under depolarized conditions, Ca 2+ entry is blocked by nifedipine and the stores diminish. Stored Ca 2+ is also greatly reduced by the blockade of SERCA with either CPA or thapsigargin. Procaine, ryanodine and caffeine blocked the store release contractions, suggesting that this involves ryanodine receptors and calcium‐induced calcium release." @default.
• W2003193484 created "2016-06-24" @default.
• W2003193484 creator A5040864474 @default.
• W2003193484 creator A5089050384 @default.
• W2003193484 date "2006-09-05" @default.
• W2003193484 modified "2023-10-16" @default.
• W2003193484 title "The role of Ca²⁺influx and intracellular Ca²⁺release in the muscarinic-mediated contraction of mammalian urinary bladder smooth muscle" @default.
• W2003193484 cites W1831556795 @default.
• W2003193484 cites W1970270099 @default.
• W2003193484 cites W1973158090 @default.
• W2003193484 cites W1974360309 @default.
• W2003193484 cites W1975700762 @default.
• W2003193484 cites W1975747269 @default.
• W2003193484 cites W1980166477 @default.
• W2003193484 cites W1989235608 @default.
• W2003193484 cites W1991410513 @default.
• W2003193484 cites W1996461970 @default.
• W2003193484 cites W2003700491 @default.
• W2003193484 cites W2010517082 @default.
• W2003193484 cites W2017353450 @default.
• W2003193484 cites W2017570722 @default.
• W2003193484 cites W2020423458 @default.
• W2003193484 cites W2024378782 @default.
• W2003193484 cites W2030700436 @default.
• W2003193484 cites W2031547337 @default.
• W2003193484 cites W2037506960 @default.
• W2003193484 cites W2060055554 @default.
• W2003193484 cites W2063627038 @default.
• W2003193484 cites W2080591875 @default.
• W2003193484 cites W2081992046 @default.
• W2003193484 cites W2084003591 @default.
• W2003193484 cites W2095325662 @default.
• W2003193484 cites W2100235197 @default.
• W2003193484 cites W2101108032 @default.
• W2003193484 cites W2128270335 @default.
• W2003193484 cites W2134696085 @default.
• W2003193484 cites W2137258759 @default.
• W2003193484 cites W2142367588 @default.
• W2003193484 cites W2150361967 @default.
• W2003193484 cites W2410802890 @default.
• W2003193484 cites W2416446817 @default.
• W2003193484 cites W2433934894 @default.
• W2003193484 doi "https://doi.org/10.1111/j.1464-410x.2006.06431.x" @default.
• W2003193484 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16978287" @default.
• W2003193484 hasPublicationYear "2006" @default.
• W2003193484 type Work @default.
• W2003193484 sameAs 2003193484 @default.
• W2003193484 citedByCount "31" @default.
• W2003193484 countsByYear W20031934842012 @default.
• W2003193484 countsByYear W20031934842013 @default.
• W2003193484 countsByYear W20031934842014 @default.
• W2003193484 countsByYear W20031934842015 @default.
• W2003193484 countsByYear W20031934842016 @default.
• W2003193484 countsByYear W20031934842018 @default.
• W2003193484 countsByYear W20031934842021 @default.
• W2003193484 countsByYear W20031934842023 @default.
• W2003193484 crossrefType "journal-article" @default.
• W2003193484 hasAuthorship W2003193484A5040864474 @default.
• W2003193484 hasAuthorship W2003193484A5089050384 @default.
• W2003193484 hasConcept C113217602 @default.
• W2003193484 hasConcept C12554922 @default.
• W2003193484 hasConcept C126322002 @default.
• W2003193484 hasConcept C134018914 @default.
• W2003193484 hasConcept C163415756 @default.
• W2003193484 hasConcept C185592680 @default.
• W2003193484 hasConcept C24998067 @default.
• W2003193484 hasConcept C2776050358 @default.
• W2003193484 hasConcept C2777146706 @default.
• W2003193484 hasConcept C2778500429 @default.
• W2003193484 hasConcept C2778988552 @default.
• W2003193484 hasConcept C2779066535 @default.
• W2003193484 hasConcept C2779762690 @default.
• W2003193484 hasConcept C2779828710 @default.
• W2003193484 hasConcept C2779996123 @default.
• W2003193484 hasConcept C2780616540 @default.
• W2003193484 hasConcept C519063684 @default.
• W2003193484 hasConcept C71924100 @default.
• W2003193484 hasConcept C86803240 @default.
• W2003193484 hasConcept C98274493 @default.
• W2003193484 hasConceptScore W2003193484C113217602 @default.
• W2003193484 hasConceptScore W2003193484C12554922 @default.
• W2003193484 hasConceptScore W2003193484C126322002 @default.
• W2003193484 hasConceptScore W2003193484C134018914 @default.
• W2003193484 hasConceptScore W2003193484C163415756 @default.
• W2003193484 hasConceptScore W2003193484C185592680 @default.
• W2003193484 hasConceptScore W2003193484C24998067 @default.
• W2003193484 hasConceptScore W2003193484C2776050358 @default.
• W2003193484 hasConceptScore W2003193484C2777146706 @default.
• W2003193484 hasConceptScore W2003193484C2778500429 @default.
• W2003193484 hasConceptScore W2003193484C2778988552 @default.
• W2003193484 hasConceptScore W2003193484C2779066535 @default.
• W2003193484 hasConceptScore W2003193484C2779762690 @default.
• W2003193484 hasConceptScore W2003193484C2779828710 @default.
• W2003193484 hasConceptScore W2003193484C2779996123 @default.
• W2003193484 hasConceptScore W2003193484C2780616540 @default.
• W2003193484 hasConceptScore W2003193484C519063684 @default.
• W2003193484 hasConceptScore W2003193484C71924100 @default.
• W2003193484 hasConceptScore W2003193484C86803240 @default.

• next