FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003202642> ?p ?o ?g. }

• W2003202642 endingPage "4308" @default.
• W2003202642 startingPage "4295" @default.
• W2003202642 abstract "The latent transforming growth factor-beta-binding protein-1 (LTBP-1) belongs to a family of extracellular glycoproteins that includes three additional isoforms (LTBP-2, -3, and -4) and the matrix proteins fibrillin-1 and -2. Originally described as a TGF-beta-masking protein, LTBP-1 is involved both in the sequestration of latent TGF-beta in the extracellular matrix and the regulation of its activation in the extracellular environment. Whereas the expression of LTBP-1 has been analyzed in normal and malignant cells and rodent and human tissues, little is known about LTBP-1 in embryonic development. To address this question, we used murine embryonic stem (ES) cells to analyze the appearance and role of LTBP-1 during ES cell differentiation. In vitro, ES cells aggregate to form embryoid bodies (EBs), which differentiate into multiple cell lineages. We analyzed LTBP-1 gene expression and LTBP-1 fiber appearance with respect to the emergence and distribution of cell types in differentiating EBs. LTBP-1 expression increased during the first 12 d in culture, appeared to remain constant between d 12 and 24, and declined thereafter. By immunostaining, fibrillar LTBP-1 was observed in those regions of the culture containing endothelial, smooth muscle, and epithelial cells. We found that inclusion of a polyclonal antibody to LTBP-1 during EB differentiation suppressed the expression of the endothelial specific genes ICAM-2 and von Willebrand factor and delayed the organization of differentiated endothelial cells into cord-like structures within the growing EBs. The same effect was observed when cultures were treated with either antibodies to TGF-beta or the latency associated peptide, which neutralize TGF-beta. Conversely, the organization of endothelial cells was enhanced by incubation with TGF-beta 1. These results suggest that during differentiation of ES cells LTBP-1 facilitates endothelial cell organization via a TGF-beta-dependent mechanism." @default.
• W2003202642 created "2016-06-24" @default.
• W2003202642 creator A5000271359 @default.
• W2003202642 creator A5034002854 @default.
• W2003202642 creator A5044889803 @default.
• W2003202642 creator A5046149280 @default.
• W2003202642 creator A5058493262 @default.
• W2003202642 creator A5020452310 @default.
• W2003202642 date "2000-12-01" @default.
• W2003202642 modified "2023-09-26" @default.
• W2003202642 title "The Latent Transforming Growth Factor-β–binding Protein-1 Promotes In Vitro Differentiation of Embryonic Stem Cells into Endothelium" @default.
• W2003202642 cites W120380984 @default.
• W2003202642 cites W1484624969 @default.
• W2003202642 cites W1491691148 @default.
• W2003202642 cites W1516075858 @default.
• W2003202642 cites W1576487484 @default.
• W2003202642 cites W1581398592 @default.
• W2003202642 cites W1606473945 @default.
• W2003202642 cites W1884021146 @default.
• W2003202642 cites W1944374750 @default.
• W2003202642 cites W1966170741 @default.
• W2003202642 cites W1966845665 @default.
• W2003202642 cites W1966908061 @default.
• W2003202642 cites W1972934993 @default.
• W2003202642 cites W1977550483 @default.
• W2003202642 cites W1978408457 @default.
• W2003202642 cites W1998975199 @default.
• W2003202642 cites W2000927180 @default.
• W2003202642 cites W2006264351 @default.
• W2003202642 cites W2006918199 @default.
• W2003202642 cites W2010814775 @default.
• W2003202642 cites W2011505677 @default.
• W2003202642 cites W2011513405 @default.
• W2003202642 cites W2011520291 @default.
• W2003202642 cites W2013701595 @default.
• W2003202642 cites W2021494076 @default.
• W2003202642 cites W2024886013 @default.
• W2003202642 cites W2025406290 @default.
• W2003202642 cites W2028528159 @default.
• W2003202642 cites W2028902486 @default.
• W2003202642 cites W2029050180 @default.
• W2003202642 cites W2033260397 @default.
• W2003202642 cites W2033926629 @default.
• W2003202642 cites W2041027285 @default.
• W2003202642 cites W2056081886 @default.
• W2003202642 cites W2060999075 @default.
• W2003202642 cites W2062457751 @default.
• W2003202642 cites W2064050439 @default.
• W2003202642 cites W2064881656 @default.
• W2003202642 cites W2070963108 @default.
• W2003202642 cites W2073903637 @default.
• W2003202642 cites W2078772718 @default.
• W2003202642 cites W2084434864 @default.
• W2003202642 cites W2085890671 @default.
• W2003202642 cites W2086833378 @default.
• W2003202642 cites W2087182361 @default.
• W2003202642 cites W2092044860 @default.
• W2003202642 cites W2093188976 @default.
• W2003202642 cites W2094165314 @default.
• W2003202642 cites W2094479420 @default.
• W2003202642 cites W2097839537 @default.
• W2003202642 cites W2098329515 @default.
• W2003202642 cites W2101314011 @default.
• W2003202642 cites W2103478108 @default.
• W2003202642 cites W2104206376 @default.
• W2003202642 cites W2116695601 @default.
• W2003202642 cites W2118199431 @default.
• W2003202642 cites W2118981873 @default.
• W2003202642 cites W2121530025 @default.
• W2003202642 cites W2135668873 @default.
• W2003202642 cites W2140036654 @default.
• W2003202642 cites W2151371344 @default.
• W2003202642 cites W2155437551 @default.
• W2003202642 cites W2158219530 @default.
• W2003202642 cites W2159228111 @default.
• W2003202642 cites W2161454032 @default.
• W2003202642 cites W2163220652 @default.
• W2003202642 cites W2294185515 @default.
• W2003202642 cites W2401772146 @default.
• W2003202642 cites W2409316477 @default.
• W2003202642 cites W2415819933 @default.
• W2003202642 cites W4229906000 @default.
• W2003202642 cites W4249215891 @default.
• W2003202642 cites W4256495668 @default.
• W2003202642 doi "https://doi.org/10.1091/mbc.11.12.4295" @default.
• W2003202642 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/15073" @default.
• W2003202642 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11102524" @default.
• W2003202642 hasPublicationYear "2000" @default.
• W2003202642 type Work @default.
• W2003202642 sameAs 2003202642 @default.
• W2003202642 citedByCount "71" @default.
• W2003202642 countsByYear W20032026422012 @default.
• W2003202642 countsByYear W20032026422013 @default.
• W2003202642 countsByYear W20032026422014 @default.
• W2003202642 countsByYear W20032026422015 @default.
• W2003202642 countsByYear W20032026422016 @default.
• W2003202642 countsByYear W20032026422017 @default.
• W2003202642 countsByYear W20032026422018 @default.

• next