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- W2003211060 abstract "Two physical methods are routinely used to determine the base composition of DNA. One measures the temperature corresponding to the midpoint of the absorbance rise (Tm) and relates it to base composition with the equation, Tm = 41 (dG + dC) + 69, the other measures buoyant density (ϱ) and relates it to base composition ϱ = 0.098(dG + dC) + 1.6535. The base composition of DNA from various sources was first determined by a chemical method and these values compared to those determined by the physical methods. Higher plants contained up to 7 mol% 5-methyldeoxycytidine in their DNA and in all cases tested deoxyguanosine = deoxycytidine + 5-methyldeoxycytidine. After determining that Tm was unaffected by the amount of 5-methyldeoxycytidine in DNA, the mol% of dA, dT, dG, and the total of dC plus 5-methyldeoxycytidine for any DNA could be calculated. Buoyant density on the other hand, was lowered 0.004 g · cm−3 for every 6.3 mol% 5-methyldeoxycytidine. Therefore, both physical parameters were related to the mole fraction of 5-methyldeoxycytidine by the following equation: 5-methyldeoxycytidine = 1.6535 − ϱ + 0.098(Tm − 6941)0.064 With a value for 5-methyldeoxycytidine an estimation of deoxycytidine was made. The resultant values agreed with the chromatographic determinations." @default.
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- W2003211060 date "1976-03-01" @default.
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- W2003211060 title "A chemical and physical method for determining the complete base composition of plant DNA" @default.
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- W2003211060 doi "https://doi.org/10.1016/0005-2787(76)90020-4" @default.
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