FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003226017> ?p ?o ?g. }

• W2003226017 endingPage "402" @default.
• W2003226017 startingPage "386" @default.
• W2003226017 abstract "Next-generation screening of disease-related metabolomic phenotypes requires monitoring of both metabolite levels and turnover rates. Stable isotope (18)O-assisted (31)P nuclear magnetic resonance (NMR) and mass spectrometry uniquely allows simultaneous measurement of phosphometabolite levels and turnover rates in tissue and blood samples. The (18)O labeling procedure is based on the incorporation of one (18)O into P(i) from [(18)O]H(2)O with each act of ATP hydrolysis and the distribution of (18)O-labeled phosphoryls among phosphate-carrying molecules. This enables simultaneous recording of ATP synthesis and utilization, phosphotransfer fluxes through adenylate kinase, creatine kinase, and glycolytic pathways, as well as mitochondrial substrate shuttle, urea and Krebs cycle activity, glycogen turnover, and intracellular energetic communication. Application of expanded (18)O-labeling procedures has revealed significant differences in the dynamics of G-6-P[(18)O] (glycolysis), G-3-P[(18)O] (substrate shuttle), and G-1-P[(18)O] (glycogenolysis) between human and rat atrial myocardium. In human atria, the turnover of G-3-P[(18)O], which defects are associated with the sudden death syndrome, was significantly higher indicating a greater importance of substrate shuttling to mitochondria. Phosphometabolomic profiling of transgenic hearts deficient in adenylate kinase (AK1-/-), which altered levels and mutations are associated to human diseases, revealed a stress-induced shift in metabolomic profile with increased CrP[(18)O] and decreased G-1-P[(18)O] metabolic dynamics. The metabolomic profile of creatine kinase M-CK/ScCKmit-/--deficient hearts is characterized by a higher G-6-[(18)O]P turnover rate, G-6-P levels, glycolytic capacity, γ/β-phosphoryl of GTP[(18)O] turnover, as well as β-[(18)O]ATP and β-[(18)O]ADP turnover, indicating altered glycolytic, guanine nucleotide, and adenylate kinase metabolic flux. Thus, (18)O-assisted gas chromatography-mass spectrometry and (31)P NMR provide a suitable platform for dynamic phosphometabolomic profiling of the cellular energetic system enabling prediction and diagnosis of metabolic diseases states." @default.
• W2003226017 created "2016-06-24" @default.
• W2003226017 creator A5009525294 @default.
• W2003226017 creator A5018675210 @default.
• W2003226017 creator A5030683638 @default.
• W2003226017 creator A5040355138 @default.
• W2003226017 creator A5042727507 @default.
• W2003226017 creator A5043681590 @default.
• W2003226017 creator A5047902810 @default.
• W2003226017 creator A5082188774 @default.
• W2003226017 date "2012-04-01" @default.
• W2003226017 modified "2023-10-14" @default.
• W2003226017 title "Dynamic phosphometabolomic profiling of human tissues and transgenic models by¹⁸O-assisted³¹P NMR and mass spectrometry" @default.
• W2003226017 cites W1030207711 @default.
• W2003226017 cites W1178260714 @default.
• W2003226017 cites W1483012720 @default.
• W2003226017 cites W1487837909 @default.
• W2003226017 cites W1497454563 @default.
• W2003226017 cites W1497946234 @default.
• W2003226017 cites W1498918598 @default.
• W2003226017 cites W1516840674 @default.
• W2003226017 cites W1550192570 @default.
• W2003226017 cites W1555165800 @default.
• W2003226017 cites W1561315996 @default.
• W2003226017 cites W1575883584 @default.
• W2003226017 cites W1583541555 @default.
• W2003226017 cites W1749560786 @default.
• W2003226017 cites W1832380510 @default.
• W2003226017 cites W187220283 @default.
• W2003226017 cites W1897503571 @default.
• W2003226017 cites W1944999268 @default.
• W2003226017 cites W1964976203 @default.
• W2003226017 cites W1968813633 @default.
• W2003226017 cites W1971754858 @default.
• W2003226017 cites W1975891365 @default.
• W2003226017 cites W1976701283 @default.
• W2003226017 cites W1978461542 @default.
• W2003226017 cites W1979721891 @default.
• W2003226017 cites W1981439819 @default.
• W2003226017 cites W1982185581 @default.
• W2003226017 cites W1983133472 @default.
• W2003226017 cites W1983602928 @default.
• W2003226017 cites W1986375719 @default.
• W2003226017 cites W1987581102 @default.
• W2003226017 cites W1988433903 @default.
• W2003226017 cites W1990712597 @default.
• W2003226017 cites W1991246779 @default.
• W2003226017 cites W1992201093 @default.
• W2003226017 cites W1992483596 @default.
• W2003226017 cites W1995783443 @default.
• W2003226017 cites W1998859334 @default.
• W2003226017 cites W2001607813 @default.
• W2003226017 cites W2005892557 @default.
• W2003226017 cites W2007545368 @default.
• W2003226017 cites W2007892976 @default.
• W2003226017 cites W2008721894 @default.
• W2003226017 cites W2009945101 @default.
• W2003226017 cites W2010449089 @default.
• W2003226017 cites W2016711181 @default.
• W2003226017 cites W2019019085 @default.
• W2003226017 cites W2019024229 @default.
• W2003226017 cites W2020325864 @default.
• W2003226017 cites W2025018219 @default.
• W2003226017 cites W2031496282 @default.
• W2003226017 cites W2033572967 @default.
• W2003226017 cites W2034289839 @default.
• W2003226017 cites W2035490018 @default.
• W2003226017 cites W2035775942 @default.
• W2003226017 cites W2038032807 @default.
• W2003226017 cites W2040195576 @default.
• W2003226017 cites W2041419269 @default.
• W2003226017 cites W2044292165 @default.
• W2003226017 cites W2045089153 @default.
• W2003226017 cites W2045185691 @default.
• W2003226017 cites W2045692760 @default.
• W2003226017 cites W2049196570 @default.
• W2003226017 cites W2051366884 @default.
• W2003226017 cites W2051974797 @default.
• W2003226017 cites W2052224618 @default.
• W2003226017 cites W2053385401 @default.
• W2003226017 cites W2053861243 @default.
• W2003226017 cites W2056781451 @default.
• W2003226017 cites W2058701510 @default.
• W2003226017 cites W2061543580 @default.
• W2003226017 cites W2061548602 @default.
• W2003226017 cites W2063523286 @default.
• W2003226017 cites W2063526545 @default.
• W2003226017 cites W2063763893 @default.
• W2003226017 cites W2068884058 @default.
• W2003226017 cites W2069046807 @default.
• W2003226017 cites W2069998681 @default.
• W2003226017 cites W2070204622 @default.
• W2003226017 cites W2072987194 @default.
• W2003226017 cites W2073039202 @default.
• W2003226017 cites W2077741640 @default.
• W2003226017 cites W2078567325 @default.
• W2003226017 cites W2079106772 @default.
• W2003226017 cites W2079212574 @default.

• next