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- W2003228029 abstract "In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. This paper describes the development of DNA markers to discriminate between Japanese Black and Fl (Japanese BlackxHolstein) breeds. The amplified fragment length polymorphism method was employed to detect candidate markers absent in Japanese Black but present in Holstein. The 1,754 primer combinations yielded eleven markers that were converted into single nucleotide polymorphism markers for high-throughput genotyping. The allele frequencies in both breeds were investigated for discrimination ability using PCR-RFLP. The probability of identifying Fl was 0.9168 and probability of misjudgment was 0.0066 using four selected markers. The markers could be useful for discriminating between Japanese Black and Fl and would contribute to the prevention of falsified breed labeling of meat." @default.
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- W2003228029 date "2006-05-25" @default.
- W2003228029 modified "2023-10-01" @default.
- W2003228029 title "Breed Discrimination Using DNA Markers Derived from AFLP in Japanese Beef Cattle" @default.
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- W2003228029 doi "https://doi.org/10.5713/ajas.2006.1106" @default.
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