Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003237425> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2003237425 endingPage "72" @default.
- W2003237425 startingPage "67" @default.
- W2003237425 abstract "We report the use of methylotrophic yeast Pichia pastoris as a host to efficiently express complement control protein repeats (CCPs) 1-4 of mouse decay accelerating factor (DAF, CD55) as a soluble protein. With this system, the mouse DAF CCP1-4-active-domain-containing module linked to a 6x His tag at its C terminus was secreted into the culture supernatant at 15 mg/L after 24 h of induction with methanol. A mouse DAF CCP1-4 mutant protein in which its two potential N-glycosylation sites were deleted by changing Asn(187) and Asn(262) to Gln was also produced. Using Ni(2+)-immobilized agarose affinity chromatography, the recombinant mouse DAF modules with their 6x His tags could be one-step isolated to SDS-PAGE purity. Polyclonal antibody against native mouse DAF CCP1-4 was raised by immunizing NZW rabbits with the purified product. Measurements of the bioactivities of the wild-type and mutant mouse DAF proteins in C3b uptake assays showed no differences in regulatory activities in either the classical or the alternative pathways. With the use of the mutant DAF protein, small rod-shaped crystals were produced and preliminary data obtained. The production of large quantities of functional recombinant mouse DAF CCP1-4 modules and their antibody offers the opportunity to study DAF structure and DAF function in vivo." @default.
- W2003237425 created "2016-06-24" @default.
- W2003237425 creator A5037641311 @default.
- W2003237425 creator A5054929780 @default.
- W2003237425 creator A5076317045 @default.
- W2003237425 creator A5088808440 @default.
- W2003237425 date "2001-09-01" @default.
- W2003237425 modified "2023-09-26" @default.
- W2003237425 title "Bulk Production and Functional Analyses of Mouse CD55's Native and Deglycosylated Active Domains" @default.
- W2003237425 cites W1537414502 @default.
- W2003237425 cites W1551650180 @default.
- W2003237425 cites W1567784487 @default.
- W2003237425 cites W1602942576 @default.
- W2003237425 cites W1895100616 @default.
- W2003237425 cites W1973605803 @default.
- W2003237425 cites W1980819109 @default.
- W2003237425 cites W2002803492 @default.
- W2003237425 cites W2008158047 @default.
- W2003237425 cites W2008765994 @default.
- W2003237425 cites W2013771339 @default.
- W2003237425 cites W2028782768 @default.
- W2003237425 cites W2042176520 @default.
- W2003237425 cites W2060807817 @default.
- W2003237425 cites W2063554961 @default.
- W2003237425 cites W2071468645 @default.
- W2003237425 cites W2077524929 @default.
- W2003237425 cites W2085974517 @default.
- W2003237425 cites W2104199760 @default.
- W2003237425 cites W2120357935 @default.
- W2003237425 cites W2152109529 @default.
- W2003237425 doi "https://doi.org/10.1006/abbi.2001.2488" @default.
- W2003237425 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11516162" @default.
- W2003237425 hasPublicationYear "2001" @default.
- W2003237425 type Work @default.
- W2003237425 sameAs 2003237425 @default.
- W2003237425 citedByCount "10" @default.
- W2003237425 countsByYear W20032374252012 @default.
- W2003237425 crossrefType "journal-article" @default.
- W2003237425 hasAuthorship W2003237425A5037641311 @default.
- W2003237425 hasAuthorship W2003237425A5054929780 @default.
- W2003237425 hasAuthorship W2003237425A5076317045 @default.
- W2003237425 hasAuthorship W2003237425A5088808440 @default.
- W2003237425 hasConcept C104317684 @default.
- W2003237425 hasConcept C111684460 @default.
- W2003237425 hasConcept C143065580 @default.
- W2003237425 hasConcept C153911025 @default.
- W2003237425 hasConcept C159654299 @default.
- W2003237425 hasConcept C179247698 @default.
- W2003237425 hasConcept C181199279 @default.
- W2003237425 hasConcept C185592680 @default.
- W2003237425 hasConcept C2777313579 @default.
- W2003237425 hasConcept C2781372317 @default.
- W2003237425 hasConcept C40767141 @default.
- W2003237425 hasConcept C54355233 @default.
- W2003237425 hasConcept C55493867 @default.
- W2003237425 hasConcept C8487232 @default.
- W2003237425 hasConcept C86803240 @default.
- W2003237425 hasConcept C956191 @default.
- W2003237425 hasConceptScore W2003237425C104317684 @default.
- W2003237425 hasConceptScore W2003237425C111684460 @default.
- W2003237425 hasConceptScore W2003237425C143065580 @default.
- W2003237425 hasConceptScore W2003237425C153911025 @default.
- W2003237425 hasConceptScore W2003237425C159654299 @default.
- W2003237425 hasConceptScore W2003237425C179247698 @default.
- W2003237425 hasConceptScore W2003237425C181199279 @default.
- W2003237425 hasConceptScore W2003237425C185592680 @default.
- W2003237425 hasConceptScore W2003237425C2777313579 @default.
- W2003237425 hasConceptScore W2003237425C2781372317 @default.
- W2003237425 hasConceptScore W2003237425C40767141 @default.
- W2003237425 hasConceptScore W2003237425C54355233 @default.
- W2003237425 hasConceptScore W2003237425C55493867 @default.
- W2003237425 hasConceptScore W2003237425C8487232 @default.
- W2003237425 hasConceptScore W2003237425C86803240 @default.
- W2003237425 hasConceptScore W2003237425C956191 @default.
- W2003237425 hasIssue "1" @default.
- W2003237425 hasLocation W20032374251 @default.
- W2003237425 hasLocation W20032374252 @default.
- W2003237425 hasOpenAccess W2003237425 @default.
- W2003237425 hasPrimaryLocation W20032374251 @default.
- W2003237425 hasRelatedWork W2374752953 @default.
- W2003237425 hasRelatedWork W2382186309 @default.
- W2003237425 hasRelatedWork W2391446476 @default.
- W2003237425 hasRelatedWork W2392089767 @default.
- W2003237425 hasRelatedWork W2392446120 @default.
- W2003237425 hasRelatedWork W2394454081 @default.
- W2003237425 hasRelatedWork W2404970265 @default.
- W2003237425 hasRelatedWork W3026200651 @default.
- W2003237425 hasRelatedWork W3030131892 @default.
- W2003237425 hasRelatedWork W2360392486 @default.
- W2003237425 hasVolume "393" @default.
- W2003237425 isParatext "false" @default.
- W2003237425 isRetracted "false" @default.
- W2003237425 magId "2003237425" @default.
- W2003237425 workType "article" @default.