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- W2003238217 abstract "A HPLC method alternative to labelled or unlabelled procedures was developed for the assay of guanylate cyclase (GC) activity. The substrate (GTP) and the product (cGMP) of the enzymatic reaction were separated in the isocratic mode on a muBondapak C18 column. The activity of GC was linearly dependent on the amount of cGMP produced in the presence of sodium nitroprusside. This approach was applied to follow the purification of GC from bovine lung and to evaluate its stability in different storage conditions." @default.
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- W2003238217 date "1997-03-01" @default.
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- W2003238217 title "Assay of soluble guanylate cyclase activity by isocratic high-performance liquid chromatography" @default.
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- W2003238217 doi "https://doi.org/10.1016/s0378-4347(96)00414-8" @default.
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