FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003242070> ?p ?o ?g. }

• W2003242070 endingPage "509" @default.
• W2003242070 startingPage "495" @default.
• W2003242070 abstract "The process of cell seeding on biocompatible scaffolds has a major impact on the morphological evolution of an engineered tissue because it involves all the key factors of tissue formation: cells, matrix, and their mutual interactions. In order to characterize the efficiency of cell seeding techniques, mainly static parameters are used such as cell density, cell distribution, and cell viability. Here, we present an experimental model that incorporates an optical density meter providing real-time information on the cell seeding velocity, a relevant dynamic parameter of cell–matrix interaction. Our setup may be adapted to fit various cell seeding protocols. A modified fluorimetric cuvette is used as bioreactor culture flask. The optical density of the magnetically stirred cell suspension is recorded by a digital optoelectronic device. We performed calibration experiments in order to prove that, in our experimental conditions, optical density depends linearly on the number of cells in the unit volume of suspension. Control studies showed that, during the time course of a typical experiment (up to 10 h), the cells (murine 3T3 fibroblasts) neither aggregated nor adhered significantly to the walls of the cuvette. Hence, our setup yields the number of cells attached to the scaffold as a function of time. In order to analyze the experimental seeding curves, we built a kinetic model based on Langmuir’s adsorption theory, which was extended to include a preliminary step of integrin function recovery. We illustrate the proposed approach by two sets of experiments that involved trypsin–EDTA or only EDTA treatment (no trypsin) used to detach the cells from the culture flasks. The data indicate that in both cases cell–matrix adhesion has a sequential, two-step dynamics, but kinetic parameters and attachment site availability depend on the experimental protocol." @default.
• W2003242070 created "2016-06-24" @default.
• W2003242070 creator A5032263096 @default.
• W2003242070 creator A5034398180 @default.
• W2003242070 creator A5039149012 @default.
• W2003242070 creator A5062009159 @default.
• W2003242070 creator A5089677576 @default.
• W2003242070 date "2008-09-02" @default.
• W2003242070 modified "2023-09-26" @default.
• W2003242070 title "The Kinetics of Cell Adhesion to Solid Scaffolds: An Experimental and Theoretical Approach" @default.
• W2003242070 cites W1540740268 @default.
• W2003242070 cites W1968799745 @default.
• W2003242070 cites W1970127412 @default.
• W2003242070 cites W1976841040 @default.
• W2003242070 cites W2018448036 @default.
• W2003242070 cites W2022825927 @default.
• W2003242070 cites W2041558541 @default.
• W2003242070 cites W2044173810 @default.
• W2003242070 cites W2060094993 @default.
• W2003242070 cites W2081312806 @default.
• W2003242070 cites W2100801711 @default.
• W2003242070 cites W2144144709 @default.
• W2003242070 cites W2145486795 @default.
• W2003242070 cites W2161118621 @default.
• W2003242070 cites W2171074980 @default.
• W2003242070 cites W88893299 @default.
• W2003242070 doi "https://doi.org/10.1007/s10867-008-9108-x" @default.
• W2003242070 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2652547" @default.
• W2003242070 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19669509" @default.
• W2003242070 hasPublicationYear "2008" @default.
• W2003242070 type Work @default.
• W2003242070 sameAs 2003242070 @default.
• W2003242070 citedByCount "19" @default.
• W2003242070 countsByYear W20032420702012 @default.
• W2003242070 countsByYear W20032420702013 @default.
• W2003242070 countsByYear W20032420702014 @default.
• W2003242070 countsByYear W20032420702016 @default.
• W2003242070 countsByYear W20032420702017 @default.
• W2003242070 countsByYear W20032420702018 @default.
• W2003242070 countsByYear W20032420702019 @default.
• W2003242070 countsByYear W20032420702021 @default.
• W2003242070 countsByYear W20032420702023 @default.
• W2003242070 crossrefType "journal-article" @default.
• W2003242070 hasAuthorship W2003242070A5032263096 @default.
• W2003242070 hasAuthorship W2003242070A5034398180 @default.
• W2003242070 hasAuthorship W2003242070A5039149012 @default.
• W2003242070 hasAuthorship W2003242070A5062009159 @default.
• W2003242070 hasAuthorship W2003242070A5089677576 @default.
• W2003242070 hasBestOaLocation W20032420702 @default.
• W2003242070 hasConcept C105341887 @default.
• W2003242070 hasConcept C106487976 @default.
• W2003242070 hasConcept C120665830 @default.
• W2003242070 hasConcept C121332964 @default.
• W2003242070 hasConcept C12554922 @default.
• W2003242070 hasConcept C136229726 @default.
• W2003242070 hasConcept C159985019 @default.
• W2003242070 hasConcept C168170006 @default.
• W2003242070 hasConcept C171250308 @default.
• W2003242070 hasConcept C178790620 @default.
• W2003242070 hasConcept C185592680 @default.
• W2003242070 hasConcept C186060115 @default.
• W2003242070 hasConcept C192562407 @default.
• W2003242070 hasConcept C202444582 @default.
• W2003242070 hasConcept C3020486325 @default.
• W2003242070 hasConcept C33923547 @default.
• W2003242070 hasConcept C36248471 @default.
• W2003242070 hasConcept C37160838 @default.
• W2003242070 hasConcept C43617362 @default.
• W2003242070 hasConcept C5961521 @default.
• W2003242070 hasConcept C71924100 @default.
• W2003242070 hasConcept C84416704 @default.
• W2003242070 hasConcept C85789140 @default.
• W2003242070 hasConcept C86803240 @default.
• W2003242070 hasConcept C97355855 @default.
• W2003242070 hasConceptScore W2003242070C105341887 @default.
• W2003242070 hasConceptScore W2003242070C106487976 @default.
• W2003242070 hasConceptScore W2003242070C120665830 @default.
• W2003242070 hasConceptScore W2003242070C121332964 @default.
• W2003242070 hasConceptScore W2003242070C12554922 @default.
• W2003242070 hasConceptScore W2003242070C136229726 @default.
• W2003242070 hasConceptScore W2003242070C159985019 @default.
• W2003242070 hasConceptScore W2003242070C168170006 @default.
• W2003242070 hasConceptScore W2003242070C171250308 @default.
• W2003242070 hasConceptScore W2003242070C178790620 @default.
• W2003242070 hasConceptScore W2003242070C185592680 @default.
• W2003242070 hasConceptScore W2003242070C186060115 @default.
• W2003242070 hasConceptScore W2003242070C192562407 @default.
• W2003242070 hasConceptScore W2003242070C202444582 @default.
• W2003242070 hasConceptScore W2003242070C3020486325 @default.
• W2003242070 hasConceptScore W2003242070C33923547 @default.
• W2003242070 hasConceptScore W2003242070C36248471 @default.
• W2003242070 hasConceptScore W2003242070C37160838 @default.
• W2003242070 hasConceptScore W2003242070C43617362 @default.
• W2003242070 hasConceptScore W2003242070C5961521 @default.
• W2003242070 hasConceptScore W2003242070C71924100 @default.
• W2003242070 hasConceptScore W2003242070C84416704 @default.
• W2003242070 hasConceptScore W2003242070C85789140 @default.
• W2003242070 hasConceptScore W2003242070C86803240 @default.

• next