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- W2003245717 abstract "Diol-bonded porous glass packing materials with average particle diameters of 5 μm and pore sizes of 170 Å and 500 Å were packed into a 25 cm × 7.2 mm I.D. column by a high-viscosity slurry-packing procedure. The number of theoretical plates ( N ) was 13 000 per column. The slurry solvent was methanol—ethylene glycol (70:30, v/v) and the packing solvent was n -heptane. The reason for using a viscous slurry solvent (2.8 cSt at 20°C) was explained by using the equation for the Reynolds number. The viscosity of the slurry solvent should be increased in proportion to the column diameter when porous glass materials are packed in a wider bore column. The value of N increased with decreasing injection volume and the optimum injection volume was 25 μl. The value of N also increased with increasing mobile phase velocity from 0.3 ml/min and the maximum was obtained around 1—2 mm/s ( N 14 500 per column and height equivalent to a theoretical plate 17.2 μm). Chromatograms and calibration graphs for polyethylene oxides, pullulans and proteins were obtained." @default.
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- W2003245717 date "1986-01-01" @default.
- W2003245717 modified "2023-09-30" @default.
- W2003245717 title "High-performance aqueous size-exclusion chromatography with diol-bonded porous glass packing materials" @default.
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- W2003245717 doi "https://doi.org/10.1016/s0021-9673(01)83740-7" @default.
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