Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003245836> ?p ?o ?g. }
- W2003245836 endingPage "3682" @default.
- W2003245836 startingPage "3668" @default.
- W2003245836 abstract "A systematic forward genetic Drosophila screen for electroretinogram mutants lacking synaptic transients identified the fuseless ( fusl ) gene, which encodes a predicted eight-pass transmembrane protein in the presynaptic membrane. Null fusl mutants display >75% reduction in evoked synaptic transmission but, conversely, an approximately threefold increase in the frequency and amplitude of spontaneous synaptic vesicle fusion events. These neurotransmission defects are rescued by a wild-type fusl transgene targeted only to the presynaptic cell, demonstrating a strictly presynaptic requirement for Fusl function. Defects in FM dye turnover at the synapse show a severely impaired exo-endo synaptic vesicle cycling pool. Consistently, ultrastructural analyses reveal accumulated vesicles arrested in clustered and docked pools at presynaptic active zones. In the absence of Fusl, calcium-dependent neurotransmitter release is dramatically compromised and there is little enhancement of synaptic efficacy with elevated external Ca 2+ concentrations. These defects are causally linked with severe loss of the Cacophony voltage-gated Ca 2+ channels, which fail to localize normally at presynaptic active zone domains in the absence of Fusl. These data indicate that Fusl regulates assembly of the presynaptic active zone Ca 2+ channel domains required for efficient coupling of the Ca 2+ influx and synaptic vesicle exocytosis during neurotransmission." @default.
- W2003245836 created "2016-06-24" @default.
- W2003245836 creator A5005496092 @default.
- W2003245836 creator A5007380594 @default.
- W2003245836 creator A5009315988 @default.
- W2003245836 creator A5014905602 @default.
- W2003245836 creator A5020524918 @default.
- W2003245836 creator A5026862562 @default.
- W2003245836 creator A5042050677 @default.
- W2003245836 creator A5072015188 @default.
- W2003245836 date "2008-04-02" @default.
- W2003245836 modified "2023-09-30" @default.
- W2003245836 title "Presynaptic Calcium Channel Localization and Calcium-Dependent Synaptic Vesicle Exocytosis Regulated by the Fuseless Protein" @default.
- W2003245836 cites W1570845414 @default.
- W2003245836 cites W1583245386 @default.
- W2003245836 cites W1593633620 @default.
- W2003245836 cites W1594082692 @default.
- W2003245836 cites W1625894348 @default.
- W2003245836 cites W1656523230 @default.
- W2003245836 cites W184718354 @default.
- W2003245836 cites W1883653169 @default.
- W2003245836 cites W1913041806 @default.
- W2003245836 cites W1945477801 @default.
- W2003245836 cites W1955231649 @default.
- W2003245836 cites W1963551844 @default.
- W2003245836 cites W1967563915 @default.
- W2003245836 cites W1969901709 @default.
- W2003245836 cites W1972484164 @default.
- W2003245836 cites W1985992145 @default.
- W2003245836 cites W1988007470 @default.
- W2003245836 cites W1990630751 @default.
- W2003245836 cites W1991650407 @default.
- W2003245836 cites W1994140164 @default.
- W2003245836 cites W1997657739 @default.
- W2003245836 cites W1999474474 @default.
- W2003245836 cites W1999772445 @default.
- W2003245836 cites W2003022978 @default.
- W2003245836 cites W2004915459 @default.
- W2003245836 cites W2008560589 @default.
- W2003245836 cites W2015651475 @default.
- W2003245836 cites W2016719838 @default.
- W2003245836 cites W2017506170 @default.
- W2003245836 cites W2023693875 @default.
- W2003245836 cites W2024881682 @default.
- W2003245836 cites W2032562864 @default.
- W2003245836 cites W2037299733 @default.
- W2003245836 cites W2040368827 @default.
- W2003245836 cites W2040866860 @default.
- W2003245836 cites W2042535445 @default.
- W2003245836 cites W2043867460 @default.
- W2003245836 cites W2047545995 @default.
- W2003245836 cites W2053050328 @default.
- W2003245836 cites W2053434084 @default.
- W2003245836 cites W2055010534 @default.
- W2003245836 cites W2057761743 @default.
- W2003245836 cites W2061340970 @default.
- W2003245836 cites W2061768568 @default.
- W2003245836 cites W2064171961 @default.
- W2003245836 cites W2064297124 @default.
- W2003245836 cites W2072847935 @default.
- W2003245836 cites W2073338554 @default.
- W2003245836 cites W2076801049 @default.
- W2003245836 cites W2078068750 @default.
- W2003245836 cites W2081834818 @default.
- W2003245836 cites W2083522206 @default.
- W2003245836 cites W2086317178 @default.
- W2003245836 cites W2089116223 @default.
- W2003245836 cites W2089689599 @default.
- W2003245836 cites W2090662268 @default.
- W2003245836 cites W2093687422 @default.
- W2003245836 cites W2096347718 @default.
- W2003245836 cites W2097012391 @default.
- W2003245836 cites W2102548112 @default.
- W2003245836 cites W2104801664 @default.
- W2003245836 cites W2110643873 @default.
- W2003245836 cites W2126084294 @default.
- W2003245836 cites W2130655302 @default.
- W2003245836 cites W2131589799 @default.
- W2003245836 cites W2133411939 @default.
- W2003245836 cites W2135710940 @default.
- W2003245836 cites W2137220579 @default.
- W2003245836 cites W2139840527 @default.
- W2003245836 cites W2144447780 @default.
- W2003245836 cites W2150764108 @default.
- W2003245836 cites W2152254886 @default.
- W2003245836 cites W2165302477 @default.
- W2003245836 cites W2166549355 @default.
- W2003245836 cites W2310340686 @default.
- W2003245836 cites W2406319787 @default.
- W2003245836 cites W4230505222 @default.
- W2003245836 cites W4243523221 @default.
- W2003245836 cites W4321317618 @default.
- W2003245836 doi "https://doi.org/10.1523/jneurosci.5553-07.2008" @default.
- W2003245836 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2769928" @default.
- W2003245836 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18385325" @default.
- W2003245836 hasPublicationYear "2008" @default.
- W2003245836 type Work @default.
- W2003245836 sameAs 2003245836 @default.