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• W2003247910 abstract "Although confocal microscopy has typically been utilized in studies of fixed specimens, its potential for exploring dynamic processes in living cells is rapidly being realized. In this report, confocal laser scanning microscopy is used to analyze the calcium wave that occurs following fertilization in living sea urchin eggs microinjected with the calcium-sensitive fluorescent probes fluo-3 or calcium green. Time-lapse recordings of optical sections depicting calcium dynamics within the eggs are also subjected to volumetric reconstructions. Such analyses indicate that (1) cytoplasmic free calcium levels become elevated throughout the fertilized egg, (2) fertilization also causes the egg nucleus to undergo a transient increase in free calcium, and (3) normal cleavage can be obtained following time-lapse imaging of the calcium waves." @default.
• W2003247910 created "2016-06-24" @default.
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• W2003247910 date "1992-02-01" @default.
• W2003247910 modified "2023-09-26" @default.
• W2003247910 title "Confocal microscopy of fertilization-induced calcium dynamics in sea urchin eggs" @default.
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• W2003247910 doi "https://doi.org/10.1016/0012-1606(92)90292-o" @default.
• W2003247910 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1730391" @default.
• W2003247910 hasPublicationYear "1992" @default.
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