Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003254757> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2003254757 endingPage "1157" @default.
- W2003254757 startingPage "1156" @default.
- W2003254757 abstract "Objective: The objective was to compare fluorochrome Hoechst 33342 (Ho342) with combined Ho342/propidium iodide (PI) stains for assessment of sperm quality. Study Design: Washed donor sperm cells were incubated in either 0, 0.15, or 15 μmol/L camptothecin (CAM) or 0.37 or 3.7 mmol/L genistein (GEN) for 4 hours at 37°C. The sperm cells were analyzed for cycle-independent apoptosis and necrosis by single- compared with dual-stained fluorescence microscopy to contrast the relative effectiveness of these two approaches. Results: The single-stain procedure did not detect viability differences (overall 76.1% ± 2.2% live). In contrast, the dual-stain procedure identified a dose-dependent decrease in viability and increased necrozoospermia for CAM and GEN treatments. Apoptosis was 2-fold higher with topoisomerase inhibitor treatment. Conclusion: The two topoisomerase inhibitors were associated with increased apoptosis and dosedependent necrosis. The data suggested that the dual-stain combination Ho342/PI was more sensitive than the single Ho342 stain analysis and permitted quantifying the apoptosis and necrosis events in sperm. (Am J Obstet Gynecol 2003;188:1156-7.)" @default.
- W2003254757 created "2016-06-24" @default.
- W2003254757 creator A5000477350 @default.
- W2003254757 creator A5017052789 @default.
- W2003254757 creator A5019331336 @default.
- W2003254757 creator A5033714520 @default.
- W2003254757 creator A5069716598 @default.
- W2003254757 date "2003-05-01" @default.
- W2003254757 modified "2023-09-26" @default.
- W2003254757 title "Dual fluorescence analysis of DNA apoptosis in sperm" @default.
- W2003254757 cites W1674816506 @default.
- W2003254757 cites W1964421187 @default.
- W2003254757 cites W1985028563 @default.
- W2003254757 cites W2074423815 @default.
- W2003254757 cites W2145352726 @default.
- W2003254757 cites W2148911589 @default.
- W2003254757 cites W2157786523 @default.
- W2003254757 cites W2162767305 @default.
- W2003254757 cites W2339361287 @default.
- W2003254757 cites W2398030213 @default.
- W2003254757 doi "https://doi.org/10.1067/mob.2003.295" @default.
- W2003254757 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12748462" @default.
- W2003254757 hasPublicationYear "2003" @default.
- W2003254757 type Work @default.
- W2003254757 sameAs 2003254757 @default.
- W2003254757 citedByCount "12" @default.
- W2003254757 countsByYear W20032547572013 @default.
- W2003254757 countsByYear W20032547572014 @default.
- W2003254757 countsByYear W20032547572015 @default.
- W2003254757 crossrefType "journal-article" @default.
- W2003254757 hasAuthorship W2003254757A5000477350 @default.
- W2003254757 hasAuthorship W2003254757A5017052789 @default.
- W2003254757 hasAuthorship W2003254757A5019331336 @default.
- W2003254757 hasAuthorship W2003254757A5033714520 @default.
- W2003254757 hasAuthorship W2003254757A5069716598 @default.
- W2003254757 hasConcept C142724271 @default.
- W2003254757 hasConcept C153911025 @default.
- W2003254757 hasConcept C16685009 @default.
- W2003254757 hasConcept C190283241 @default.
- W2003254757 hasConcept C2775934118 @default.
- W2003254757 hasConcept C2779682216 @default.
- W2003254757 hasConcept C2781087480 @default.
- W2003254757 hasConcept C2781294515 @default.
- W2003254757 hasConcept C31573885 @default.
- W2003254757 hasConcept C503630168 @default.
- W2003254757 hasConcept C55493867 @default.
- W2003254757 hasConcept C71924100 @default.
- W2003254757 hasConcept C74864618 @default.
- W2003254757 hasConcept C86803240 @default.
- W2003254757 hasConceptScore W2003254757C142724271 @default.
- W2003254757 hasConceptScore W2003254757C153911025 @default.
- W2003254757 hasConceptScore W2003254757C16685009 @default.
- W2003254757 hasConceptScore W2003254757C190283241 @default.
- W2003254757 hasConceptScore W2003254757C2775934118 @default.
- W2003254757 hasConceptScore W2003254757C2779682216 @default.
- W2003254757 hasConceptScore W2003254757C2781087480 @default.
- W2003254757 hasConceptScore W2003254757C2781294515 @default.
- W2003254757 hasConceptScore W2003254757C31573885 @default.
- W2003254757 hasConceptScore W2003254757C503630168 @default.
- W2003254757 hasConceptScore W2003254757C55493867 @default.
- W2003254757 hasConceptScore W2003254757C71924100 @default.
- W2003254757 hasConceptScore W2003254757C74864618 @default.
- W2003254757 hasConceptScore W2003254757C86803240 @default.
- W2003254757 hasIssue "5" @default.
- W2003254757 hasLocation W20032547571 @default.
- W2003254757 hasLocation W20032547572 @default.
- W2003254757 hasOpenAccess W2003254757 @default.
- W2003254757 hasPrimaryLocation W20032547571 @default.
- W2003254757 hasRelatedWork W2003254757 @default.
- W2003254757 hasRelatedWork W2014030933 @default.
- W2003254757 hasRelatedWork W2044152887 @default.
- W2003254757 hasRelatedWork W2044789787 @default.
- W2003254757 hasRelatedWork W2055586490 @default.
- W2003254757 hasRelatedWork W2097997168 @default.
- W2003254757 hasRelatedWork W2139623236 @default.
- W2003254757 hasRelatedWork W2163033081 @default.
- W2003254757 hasRelatedWork W2328524531 @default.
- W2003254757 hasRelatedWork W2386969419 @default.
- W2003254757 hasVolume "188" @default.
- W2003254757 isParatext "false" @default.
- W2003254757 isRetracted "false" @default.
- W2003254757 magId "2003254757" @default.
- W2003254757 workType "article" @default.