FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003258294> ?p ?o ?g. }

• W2003258294 endingPage "28525" @default.
• W2003258294 startingPage "28518" @default.
• W2003258294 abstract "Accurate translation of mRNA into protein is a fundamental biological process critical for maintaining normal cellular functions. To ensure translational fidelity, aminoacyl-tRNA synthetases (aaRSs) employ pre-transfer and post-transfer editing activities to hydrolyze misactivated and mischarged amino acids, respectively. Whereas post-transfer editing, which requires either a specialized domain in aaRS or a trans-protein factor, is well described, the mechanism of pre-transfer editing is less understood. Here, we show that yeast mitochondrial threonyl-tRNA synthetase (MST1), which lacks an editing domain, utilizes pre-transfer editing to discriminate against serine. MST1 misactivates serine and edits seryl adenylate (Ser-AMP) in a tRNA-independent manner. MST1 hydrolyzes 80% of misactivated Ser-AMP at a rate 4-fold higher than that for the cognate threonyl adenylate (Thr-AMP) while releasing 20% of Ser-AMP into the solution. To understand the mechanism of pre-transfer editing, we solved the crystal structure of MST1 complexed with an analog of Ser-AMP. The binding of the Ser-AMP analog to MST1 induces conformational changes in the aminoacylation active site, and it positions a potential hydrolytic water molecule more favorably for nucleophilic attack. In addition, inhibition results reveal that the Ser-AMP analog binds the active site 100-fold less tightly than the Thr-AMP analog. In conclusion, we propose that the plasticity of the aminoacylation site in MST1 allows binding of Ser-AMP and the appropriate positioning of the hydrolytic water molecule." @default.
• W2003258294 created "2016-06-24" @default.
• W2003258294 creator A5001421773 @default.
• W2003258294 creator A5013517453 @default.
• W2003258294 creator A5047797998 @default.
• W2003258294 creator A5063450287 @default.
• W2003258294 creator A5087115387 @default.
• W2003258294 date "2012-08-01" @default.
• W2003258294 modified "2023-10-05" @default.
• W2003258294 title "The Mechanism of Pre-transfer Editing in Yeast Mitochondrial Threonyl-tRNA Synthetase" @default.
• W2003258294 cites W1480195325 @default.
• W2003258294 cites W1539796472 @default.
• W2003258294 cites W1549833690 @default.
• W2003258294 cites W1965294448 @default.
• W2003258294 cites W1967286437 @default.
• W2003258294 cites W1976678081 @default.
• W2003258294 cites W1978453601 @default.
• W2003258294 cites W1982177211 @default.
• W2003258294 cites W2002895409 @default.
• W2003258294 cites W2002944601 @default.
• W2003258294 cites W2007239250 @default.
• W2003258294 cites W2008676983 @default.
• W2003258294 cites W2010512527 @default.
• W2003258294 cites W2013165826 @default.
• W2003258294 cites W2016059768 @default.
• W2003258294 cites W2016485550 @default.
• W2003258294 cites W2018793220 @default.
• W2003258294 cites W2019785852 @default.
• W2003258294 cites W2031465355 @default.
• W2003258294 cites W2034108516 @default.
• W2003258294 cites W2039790548 @default.
• W2003258294 cites W2041034033 @default.
• W2003258294 cites W2043938667 @default.
• W2003258294 cites W2053811117 @default.
• W2003258294 cites W2063309485 @default.
• W2003258294 cites W2064580738 @default.
• W2003258294 cites W2066392481 @default.
• W2003258294 cites W2077454499 @default.
• W2003258294 cites W2078707325 @default.
• W2003258294 cites W2082957812 @default.
• W2003258294 cites W2085863852 @default.
• W2003258294 cites W2089143068 @default.
• W2003258294 cites W2091067056 @default.
• W2003258294 cites W2120744038 @default.
• W2003258294 cites W2124026197 @default.
• W2003258294 cites W2125768570 @default.
• W2003258294 cites W2137532652 @default.
• W2003258294 cites W2140885472 @default.
• W2003258294 cites W2144081223 @default.
• W2003258294 cites W2147488317 @default.
• W2003258294 cites W2163341755 @default.
• W2003258294 cites W2180229411 @default.
• W2003258294 cites W2315564388 @default.
• W2003258294 doi "https://doi.org/10.1074/jbc.m112.372920" @default.
• W2003258294 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3436575" @default.
• W2003258294 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22773845" @default.
• W2003258294 hasPublicationYear "2012" @default.
• W2003258294 type Work @default.
• W2003258294 sameAs 2003258294 @default.
• W2003258294 citedByCount "31" @default.
• W2003258294 countsByYear W20032582942013 @default.
• W2003258294 countsByYear W20032582942014 @default.
• W2003258294 countsByYear W20032582942015 @default.
• W2003258294 countsByYear W20032582942016 @default.
• W2003258294 countsByYear W20032582942017 @default.
• W2003258294 countsByYear W20032582942020 @default.
• W2003258294 countsByYear W20032582942022 @default.
• W2003258294 countsByYear W20032582942023 @default.
• W2003258294 crossrefType "journal-article" @default.
• W2003258294 hasAuthorship W2003258294A5001421773 @default.
• W2003258294 hasAuthorship W2003258294A5013517453 @default.
• W2003258294 hasAuthorship W2003258294A5047797998 @default.
• W2003258294 hasAuthorship W2003258294A5063450287 @default.
• W2003258294 hasAuthorship W2003258294A5087115387 @default.
• W2003258294 hasBestOaLocation W20032582941 @default.
• W2003258294 hasConcept C104317684 @default.
• W2003258294 hasConcept C105580179 @default.
• W2003258294 hasConcept C11960822 @default.
• W2003258294 hasConcept C149364088 @default.
• W2003258294 hasConcept C151238385 @default.
• W2003258294 hasConcept C153957851 @default.
• W2003258294 hasConcept C180526460 @default.
• W2003258294 hasConcept C181199279 @default.
• W2003258294 hasConcept C185592680 @default.
• W2003258294 hasConcept C2776414213 @default.
• W2003258294 hasConcept C2779315393 @default.
• W2003258294 hasConcept C2910418148 @default.
• W2003258294 hasConcept C55493867 @default.
• W2003258294 hasConcept C67705224 @default.
• W2003258294 hasConcept C86803240 @default.
• W2003258294 hasConceptScore W2003258294C104317684 @default.
• W2003258294 hasConceptScore W2003258294C105580179 @default.
• W2003258294 hasConceptScore W2003258294C11960822 @default.
• W2003258294 hasConceptScore W2003258294C149364088 @default.
• W2003258294 hasConceptScore W2003258294C151238385 @default.
• W2003258294 hasConceptScore W2003258294C153957851 @default.
• W2003258294 hasConceptScore W2003258294C180526460 @default.
• W2003258294 hasConceptScore W2003258294C181199279 @default.

• next