Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003264895> ?p ?o ?g. }
- W2003264895 endingPage "2655" @default.
- W2003264895 startingPage "2647" @default.
- W2003264895 abstract "Apples contain significant amounts of flavonoids that are potentially cancer risk reducing by acting antioxidative or antiproliferative and by favorably modulating gene expression. The purpose of this study was to investigate whether polyphenols from apples modulate expression of genes related to colon cancer prevention in preneoplastic cells derived from colon adenoma (LT97). For this, LT97 cells were treated with effective concentrations of apple extracts (AEs). RNA was isolated and used for synthesis and labeling of cDNA that was hybridized to cDNA-arrays. Gene expression studies were performed using a commercial cDNA-array from Superarray® that contains a limited number of genes (96 genes) related to drug metabolism, and a custom-made cDNA microarray that contains a higher number of genes (300 genes, including some genes from Superarray) related to mechanisms of carcinogenesis or chemoprevention. Real-time PCR and enzyme activity assays were additionally performed to confirm selected array results. Treatment of cells with AE resulted in 30 and 46 genes expressed over cut-off values (≥1.5- or ≤0.7-fold) in Superarray and custom array, respectively. Of 87 genes spotted on both arrays, 4 genes (CYP3A7, CYP4F3, CHST7, GSTT2) were regulated with similar directional changes. Expression of selected phase II genes (GSTP1, GSTT2, GSTA4, UGT1A1, UGT2B7), regulated on either array, was confirmed by real-time PCR. The enzyme activities of glutathione S-transferases and UDP-glucuronosyltransferases were altered by treatment of LT97 cells with AE. The observed altered gene expression patterns in LT97 cells, resulting from AE treatment, points to a possible protection of the cells against some toxicological insults. © 2008 Wiley-Liss, Inc." @default.
- W2003264895 created "2016-06-24" @default.
- W2003264895 creator A5000799578 @default.
- W2003264895 creator A5012411041 @default.
- W2003264895 creator A5022510637 @default.
- W2003264895 creator A5036137819 @default.
- W2003264895 creator A5045413165 @default.
- W2003264895 creator A5047497411 @default.
- W2003264895 creator A5061319253 @default.
- W2003264895 creator A5073186782 @default.
- W2003264895 creator A5073771270 @default.
- W2003264895 date "2008-01-01" @default.
- W2003264895 modified "2023-09-28" @default.
- W2003264895 title "Apple polyphenols modulate expression of selected genes related to toxicological defence and stress response in human colon adenoma cells" @default.
- W2003264895 cites W144983782 @default.
- W2003264895 cites W1545965226 @default.
- W2003264895 cites W1581414347 @default.
- W2003264895 cites W1654089893 @default.
- W2003264895 cites W1977264422 @default.
- W2003264895 cites W1985491442 @default.
- W2003264895 cites W1993129579 @default.
- W2003264895 cites W1998874387 @default.
- W2003264895 cites W2003877355 @default.
- W2003264895 cites W2014418136 @default.
- W2003264895 cites W2015519364 @default.
- W2003264895 cites W2019275065 @default.
- W2003264895 cites W2019364516 @default.
- W2003264895 cites W2023196187 @default.
- W2003264895 cites W2025071869 @default.
- W2003264895 cites W2029784055 @default.
- W2003264895 cites W2030364043 @default.
- W2003264895 cites W2031980384 @default.
- W2003264895 cites W2038906343 @default.
- W2003264895 cites W2040631816 @default.
- W2003264895 cites W2053619526 @default.
- W2003264895 cites W2060066334 @default.
- W2003264895 cites W2079414582 @default.
- W2003264895 cites W2081187091 @default.
- W2003264895 cites W2084494768 @default.
- W2003264895 cites W2092339733 @default.
- W2003264895 cites W2094613397 @default.
- W2003264895 cites W2105869334 @default.
- W2003264895 cites W2117906220 @default.
- W2003264895 cites W2121793916 @default.
- W2003264895 cites W2127892423 @default.
- W2003264895 cites W2128370109 @default.
- W2003264895 cites W2128878512 @default.
- W2003264895 cites W2132285473 @default.
- W2003264895 cites W2133450689 @default.
- W2003264895 cites W2134536765 @default.
- W2003264895 cites W2139288503 @default.
- W2003264895 cites W2141009231 @default.
- W2003264895 cites W2141956082 @default.
- W2003264895 cites W2158944838 @default.
- W2003264895 cites W2166002104 @default.
- W2003264895 cites W4238581030 @default.
- W2003264895 cites W4293247451 @default.
- W2003264895 doi "https://doi.org/10.1002/ijc.23440" @default.
- W2003264895 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18351577" @default.
- W2003264895 hasPublicationYear "2008" @default.
- W2003264895 type Work @default.
- W2003264895 sameAs 2003264895 @default.
- W2003264895 citedByCount "30" @default.
- W2003264895 countsByYear W20032648952012 @default.
- W2003264895 countsByYear W20032648952013 @default.
- W2003264895 countsByYear W20032648952014 @default.
- W2003264895 countsByYear W20032648952015 @default.
- W2003264895 countsByYear W20032648952016 @default.
- W2003264895 countsByYear W20032648952017 @default.
- W2003264895 countsByYear W20032648952018 @default.
- W2003264895 countsByYear W20032648952019 @default.
- W2003264895 countsByYear W20032648952020 @default.
- W2003264895 countsByYear W20032648952021 @default.
- W2003264895 crossrefType "journal-article" @default.
- W2003264895 hasAuthorship W2003264895A5000799578 @default.
- W2003264895 hasAuthorship W2003264895A5012411041 @default.
- W2003264895 hasAuthorship W2003264895A5022510637 @default.
- W2003264895 hasAuthorship W2003264895A5036137819 @default.
- W2003264895 hasAuthorship W2003264895A5045413165 @default.
- W2003264895 hasAuthorship W2003264895A5047497411 @default.
- W2003264895 hasAuthorship W2003264895A5061319253 @default.
- W2003264895 hasAuthorship W2003264895A5073186782 @default.
- W2003264895 hasAuthorship W2003264895A5073771270 @default.
- W2003264895 hasBestOaLocation W20032648951 @default.
- W2003264895 hasConcept C104317684 @default.
- W2003264895 hasConcept C135763542 @default.
- W2003264895 hasConcept C150194340 @default.
- W2003264895 hasConcept C153911025 @default.
- W2003264895 hasConcept C181199279 @default.
- W2003264895 hasConcept C186836561 @default.
- W2003264895 hasConcept C187882448 @default.
- W2003264895 hasConcept C2776907368 @default.
- W2003264895 hasConcept C2780122483 @default.
- W2003264895 hasConcept C48023723 @default.
- W2003264895 hasConcept C502942594 @default.
- W2003264895 hasConcept C538909803 @default.
- W2003264895 hasConcept C54355233 @default.
- W2003264895 hasConcept C55493867 @default.