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- W2003264913 abstract "PG9 is the founder member of an expanding family of glycan-dependent human antibodies that preferentially bind the HIV (HIV-1) envelope (Env) glycoprotein (gp) trimer and broadly neutralize the virus. Here, we show that a soluble SOSIP.664 gp140 trimer constructed from the Clade A BG505 sequence binds PG9 with high affinity (∼11 nM), enabling structural and biophysical characterizations of the PG9:Env trimer complex. The BG505 SOSIP.664 gp140 trimer is remarkably stable as assessed by electron microscopy (EM) and differential scanning calorimetry. EM, small angle X-ray scattering, size exclusion chromatography with inline multiangle light scattering and isothermal titration calorimetry all indicate that only a single PG9 fragment antigen-binding (Fab) binds to the Env trimer. An ∼18 Å EM reconstruction demonstrates that PG9 recognizes the trimer asymmetrically at its apex via contact with two of the three gp120 protomers, possibly contributing to its reported preference for a quaternary epitope. Molecular modeling and isothermal titration calorimetry binding experiments with an engineered PG9 mutant suggest that, in addition to the N156 and N160 glycan interactions observed in crystal structures of PG9 with a scaffolded V1/V2 domain, PG9 makes secondary interactions with an N160 glycan from an adjacent gp120 protomer in the antibody–trimer complex. Together, these structural and biophysical findings should facilitate the design of HIV-1 immunogens that possess all elements of the quaternary PG9 epitope required to induce broadly neutralizing antibodies against this region." @default.
- W2003264913 created "2016-06-24" @default.
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- W2003264913 date "2013-02-20" @default.
- W2003264913 modified "2023-10-14" @default.
- W2003264913 title "Asymmetric recognition of the HIV-1 trimer by broadly neutralizing antibody PG9" @default.
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- W2003264913 cites W1984150281 @default.
- W2003264913 cites W1986103819 @default.
- W2003264913 cites W1990745725 @default.
- W2003264913 cites W1991357892 @default.
- W2003264913 cites W1998406600 @default.
- W2003264913 cites W2000475809 @default.
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- W2003264913 cites W2008985095 @default.
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- W2003264913 cites W2015721441 @default.
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- W2003264913 cites W2016453064 @default.
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- W2003264913 cites W2029353650 @default.
- W2003264913 cites W2032140209 @default.
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- W2003264913 doi "https://doi.org/10.1073/pnas.1217537110" @default.
- W2003264913 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3600498" @default.
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