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• W2003294189 abstract "Glycolytic gene expression is mediated by the Gcr1p‐Gcr2p transcriptional activation complex. A screen for multicopy suppressors of gcr2 yielded SGC1 . SGC1 's suppression activity was specific to gcr2 , it did not extend to gcr1 . Disruption of SGC1 moderately affected glycolytic enzyme activities, although no growth defect was evident. Sgc1p exhibits a bHLH motif which is characteristic of E‐box DNA‐binding proteins. DNA footprinting experiments demonstrated Sgc1p's ability to bind at an E‐box. However, its binding specificity was less than 10‐fold, which is also characteristic of E‐box binding proteins. LexA fusion experiments demonstrated that Sgc1p has weak intrinsic activating activity independent of GCR1 and GCR2 . We propose that Sgc1p binds at E‐boxes of glycolytic genes and contributes to their activation." @default.
• W2003294189 created "2016-06-24" @default.
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• W2003294189 date "1999-12-17" @default.
• W2003294189 modified "2023-10-15" @default.
• W2003294189 title "The E‐box DNA binding protein Sgc1p suppresses the <i>gcr2</i> mutation, which is involved in transcriptional activation of glycolytic genes in <i>Saccharomyces cerevisiae</i>" @default.
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• W2003294189 doi "https://doi.org/10.1016/s0014-5793(99)01654-3" @default.
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