Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003295354> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2003295354 endingPage "1165" @default.
- W2003295354 startingPage "1160" @default.
- W2003295354 abstract "In a previous study, we developed a novel cationic lipid consisting of polyamidoamine dendron of third generation and two dodecyl chains, designated as DL-G3, which in combination with a fusogenic lipid dioleoylphosphatidylethanolamine (DOPE) achieves efficient transfection of CV1 cells by synergetic action of the proton sponge effect and membrane fusion. This study examines the effect of serum on the transfection activity of the DL-G3-DOPE-plasmid DNA lipoplexes. The transfection activity of a lipoplex with a composition optimized in the absence of serum decreased markedly in the presence of serum. However, the lipoplexes that induce efficient transfection in the presence of serum were obtainable by controlling the charge ratio of the primary amine of the DL-G3 to the phosphate group (N/P ratio) and DOPE content. The complex, which exhibited the highest transfection activity in the presence of serum, has a lower N/P ratio and higher DOPE content than that optimized in the absence of serum. Whereas disintegration of these complexes was induced by addition of heparin, which is a polysaccharide with negatively charged groups, the complex that retained transfection activity in the presence of serum required more negative charges of heparin for complex disintegration. That result implies its higher stability against negatively charged serum proteins. Comparison of the serum-resistant complex with some commercially available transfection reagents, such as Lipofectamine and SuperFect, indicates that the DL-G3 complex achieved more efficient transfection of these cells in the presence of serum." @default.
- W2003295354 created "2016-06-24" @default.
- W2003295354 creator A5029199190 @default.
- W2003295354 creator A5031924275 @default.
- W2003295354 creator A5040666669 @default.
- W2003295354 creator A5087299748 @default.
- W2003295354 date "2005-08-27" @default.
- W2003295354 modified "2023-09-27" @default.
- W2003295354 title "Preparation of Efficient Gene Carriers Using a Polyamidoamine Dendron-Bearing Lipid: Improvement of Serum Resistance" @default.
- W2003295354 cites W1992557609 @default.
- W2003295354 cites W1997818739 @default.
- W2003295354 cites W2009508594 @default.
- W2003295354 cites W2034342858 @default.
- W2003295354 cites W2036732463 @default.
- W2003295354 cites W2044637168 @default.
- W2003295354 cites W2062412252 @default.
- W2003295354 cites W2063682682 @default.
- W2003295354 cites W2126201141 @default.
- W2003295354 cites W2315572179 @default.
- W2003295354 doi "https://doi.org/10.1021/bc050012f" @default.
- W2003295354 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16173793" @default.
- W2003295354 hasPublicationYear "2005" @default.
- W2003295354 type Work @default.
- W2003295354 sameAs 2003295354 @default.
- W2003295354 citedByCount "48" @default.
- W2003295354 countsByYear W20032953542012 @default.
- W2003295354 countsByYear W20032953542013 @default.
- W2003295354 countsByYear W20032953542014 @default.
- W2003295354 countsByYear W20032953542015 @default.
- W2003295354 countsByYear W20032953542016 @default.
- W2003295354 countsByYear W20032953542017 @default.
- W2003295354 countsByYear W20032953542020 @default.
- W2003295354 countsByYear W20032953542022 @default.
- W2003295354 crossrefType "journal-article" @default.
- W2003295354 hasAuthorship W2003295354A5029199190 @default.
- W2003295354 hasAuthorship W2003295354A5031924275 @default.
- W2003295354 hasAuthorship W2003295354A5040666669 @default.
- W2003295354 hasAuthorship W2003295354A5087299748 @default.
- W2003295354 hasConcept C104317684 @default.
- W2003295354 hasConcept C12554922 @default.
- W2003295354 hasConcept C153911025 @default.
- W2003295354 hasConcept C155887181 @default.
- W2003295354 hasConcept C183882617 @default.
- W2003295354 hasConcept C185592680 @default.
- W2003295354 hasConcept C188027245 @default.
- W2003295354 hasConcept C40767141 @default.
- W2003295354 hasConcept C49923114 @default.
- W2003295354 hasConcept C54009773 @default.
- W2003295354 hasConcept C55493867 @default.
- W2003295354 hasConcept C55637122 @default.
- W2003295354 hasConcept C86803240 @default.
- W2003295354 hasConcept C92087593 @default.
- W2003295354 hasConceptScore W2003295354C104317684 @default.
- W2003295354 hasConceptScore W2003295354C12554922 @default.
- W2003295354 hasConceptScore W2003295354C153911025 @default.
- W2003295354 hasConceptScore W2003295354C155887181 @default.
- W2003295354 hasConceptScore W2003295354C183882617 @default.
- W2003295354 hasConceptScore W2003295354C185592680 @default.
- W2003295354 hasConceptScore W2003295354C188027245 @default.
- W2003295354 hasConceptScore W2003295354C40767141 @default.
- W2003295354 hasConceptScore W2003295354C49923114 @default.
- W2003295354 hasConceptScore W2003295354C54009773 @default.
- W2003295354 hasConceptScore W2003295354C55493867 @default.
- W2003295354 hasConceptScore W2003295354C55637122 @default.
- W2003295354 hasConceptScore W2003295354C86803240 @default.
- W2003295354 hasConceptScore W2003295354C92087593 @default.
- W2003295354 hasIssue "5" @default.
- W2003295354 hasLocation W20032953541 @default.
- W2003295354 hasLocation W20032953542 @default.
- W2003295354 hasOpenAccess W2003295354 @default.
- W2003295354 hasPrimaryLocation W20032953541 @default.
- W2003295354 hasRelatedWork W2003295354 @default.
- W2003295354 hasRelatedWork W2048897010 @default.
- W2003295354 hasRelatedWork W2066939729 @default.
- W2003295354 hasRelatedWork W2073581953 @default.
- W2003295354 hasRelatedWork W2124393213 @default.
- W2003295354 hasRelatedWork W2165398062 @default.
- W2003295354 hasRelatedWork W2310041894 @default.
- W2003295354 hasRelatedWork W2360482012 @default.
- W2003295354 hasRelatedWork W2386742839 @default.
- W2003295354 hasRelatedWork W2393933126 @default.
- W2003295354 hasVolume "16" @default.
- W2003295354 isParatext "false" @default.
- W2003295354 isRetracted "false" @default.
- W2003295354 magId "2003295354" @default.
- W2003295354 workType "article" @default.