FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003326994> ?p ?o ?g. }

• W2003326994 endingPage "1142" @default.
• W2003326994 startingPage "1134" @default.
• W2003326994 abstract "The reduction of the aflatoxin B1 (AFB1) dialdehyde metabolite to its corresponding mono and dialcohols, catalyzed by aflatoxin B1-aldehyde reductase (AFAR, rat AKR7A1, and human AKR7A3), is greatly increased in livers of rats treated with numerous chemoprotective agents. Recombinant human AKR7A3 has been shown to reduce the AFB1-dialdehyde at rates greater than those of the rat AKR7A1. The activity of AKR7A1 or AKR7A3 may detoxify the AFB1-dialdehyde, which reacts with proteins, and thereby inhibits AFB1-induced toxicity; however, direct experimental evidence of this hypothesis was lacking. Two human B lymphoblastoid cell lines, designated pMF6/1A2/AKR7A1 and pMF6/1A2, were genetically engineered to stably express AKR7A1 and/or cytochrome P4501A2 (1A2). The pMF6/1A2/AKR7A1 cells were refractory to the cytotoxic effects of 3 ng/mL AFB1, in comparison to pM6/1A2 cells, which were more sensitive. Diminished protection occurred at higher concentrations of AFB1 in pMF6/1A2/AKR7A1 cells, suggesting that additional factors were influencing cell survival. COS-7 cells were transfected with either vector control, rat AKR7A1, or human AKR7A3, and the cells were treated with AFB1-dialdehyde. There was a 6-fold increase in the dialdehyde LC50, from 66 µM in vector-transfected cells to 400 µM in AKR7A1-transfected cells, and an 8.5-fold increase from 35 µM in vector-transfected cells to 300 µM in AKR7A3-transfected cells. In both cases, this protective effect of the AFAR enzyme was accompanied by a marked decrease in protein adducts. Fractionation of the cellular protein showed that the mitochondria/nuclei and microsomal fractions contained the highest concentration of protein adducts. The levels of human AKR7A3 and AKR7A2 were measured in 12 human liver samples. The expression of AKR7A3 was detectable in all livers and lower than those of AKR7A2 in 11 of the 12 samples. Overall, these results provide the first direct evidence of a role for rat AKR7A1 and human AKR7A3 in protection against AFB1-induced cytotoxicity and protein adduct formation." @default.
• W2003326994 created "2016-06-24" @default.
• W2003326994 creator A5013703078 @default.
• W2003326994 creator A5018650467 @default.
• W2003326994 creator A5030684056 @default.
• W2003326994 creator A5058389506 @default.
• W2003326994 creator A5058407840 @default.
• W2003326994 creator A5064617617 @default.
• W2003326994 creator A5072089972 @default.
• W2003326994 creator A5078418860 @default.
• W2003326994 creator A5084365721 @default.
• W2003326994 date "2008-04-15" @default.
• W2003326994 modified "2023-09-23" @default.
• W2003326994 title "Protection Against Aflatoxin B₁-Induced Cytotoxicity by Expression of the Cloned Aflatoxin B₁-Aldehyde Reductases Rat AKR7A1 and Human AKR7A3" @default.
• W2003326994 cites W102494646 @default.
• W2003326994 cites W1846205077 @default.
• W2003326994 cites W1964580600 @default.
• W2003326994 cites W1989901743 @default.
• W2003326994 cites W1990928842 @default.
• W2003326994 cites W1998483947 @default.
• W2003326994 cites W1999057078 @default.
• W2003326994 cites W2002502104 @default.
• W2003326994 cites W2012690460 @default.
• W2003326994 cites W2016849729 @default.
• W2003326994 cites W2035827113 @default.
• W2003326994 cites W2038124483 @default.
• W2003326994 cites W2040057136 @default.
• W2003326994 cites W2044717712 @default.
• W2003326994 cites W2053293903 @default.
• W2003326994 cites W2063140886 @default.
• W2003326994 cites W2064752311 @default.
• W2003326994 cites W2067549443 @default.
• W2003326994 cites W2070195393 @default.
• W2003326994 cites W2071417758 @default.
• W2003326994 cites W2078936452 @default.
• W2003326994 cites W2079531334 @default.
• W2003326994 cites W2079750936 @default.
• W2003326994 cites W2082293555 @default.
• W2003326994 cites W2083810475 @default.
• W2003326994 cites W2084193227 @default.
• W2003326994 cites W2086174788 @default.
• W2003326994 cites W2100837269 @default.
• W2003326994 cites W2105494972 @default.
• W2003326994 cites W2114169043 @default.
• W2003326994 cites W2152554398 @default.
• W2003326994 cites W2336487486 @default.
• W2003326994 cites W4294216491 @default.
• W2003326994 doi "https://doi.org/10.1021/tx7004458" @default.
• W2003326994 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3838790" @default.
• W2003326994 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18416522" @default.
• W2003326994 hasPublicationYear "2008" @default.
• W2003326994 type Work @default.
• W2003326994 sameAs 2003326994 @default.
• W2003326994 citedByCount "31" @default.
• W2003326994 countsByYear W20033269942012 @default.
• W2003326994 countsByYear W20033269942013 @default.
• W2003326994 countsByYear W20033269942014 @default.
• W2003326994 countsByYear W20033269942015 @default.
• W2003326994 countsByYear W20033269942016 @default.
• W2003326994 countsByYear W20033269942017 @default.
• W2003326994 countsByYear W20033269942018 @default.
• W2003326994 countsByYear W20033269942019 @default.
• W2003326994 countsByYear W20033269942020 @default.
• W2003326994 countsByYear W20033269942021 @default.
• W2003326994 countsByYear W20033269942023 @default.
• W2003326994 crossrefType "journal-article" @default.
• W2003326994 hasAuthorship W2003326994A5013703078 @default.
• W2003326994 hasAuthorship W2003326994A5018650467 @default.
• W2003326994 hasAuthorship W2003326994A5030684056 @default.
• W2003326994 hasAuthorship W2003326994A5058389506 @default.
• W2003326994 hasAuthorship W2003326994A5058407840 @default.
• W2003326994 hasAuthorship W2003326994A5064617617 @default.
• W2003326994 hasAuthorship W2003326994A5072089972 @default.
• W2003326994 hasAuthorship W2003326994A5078418860 @default.
• W2003326994 hasAuthorship W2003326994A5084365721 @default.
• W2003326994 hasBestOaLocation W20033269942 @default.
• W2003326994 hasConcept C104317684 @default.
• W2003326994 hasConcept C109316439 @default.
• W2003326994 hasConcept C153911025 @default.
• W2003326994 hasConcept C185592680 @default.
• W2003326994 hasConcept C202751555 @default.
• W2003326994 hasConcept C2777477808 @default.
• W2003326994 hasConcept C31903555 @default.
• W2003326994 hasConcept C40758303 @default.
• W2003326994 hasConcept C54009773 @default.
• W2003326994 hasConcept C54355233 @default.
• W2003326994 hasConcept C55493867 @default.
• W2003326994 hasConcept C81885089 @default.
• W2003326994 hasConcept C86803240 @default.
• W2003326994 hasConceptScore W2003326994C104317684 @default.
• W2003326994 hasConceptScore W2003326994C109316439 @default.
• W2003326994 hasConceptScore W2003326994C153911025 @default.
• W2003326994 hasConceptScore W2003326994C185592680 @default.
• W2003326994 hasConceptScore W2003326994C202751555 @default.
• W2003326994 hasConceptScore W2003326994C2777477808 @default.
• W2003326994 hasConceptScore W2003326994C31903555 @default.
• W2003326994 hasConceptScore W2003326994C40758303 @default.
• W2003326994 hasConceptScore W2003326994C54009773 @default.

• next