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• W2003334953 abstract "Article17 January 2000free access Identification of an erythroid-enriched endoribonuclease activity involved in specific mRNA cleavage Zuoren Wang Zuoren Wang Rutgers University, Department of Cell Biology and Neuroscience, 604 Allison Road, Piscataway, NJ, 08854-8082 USA Search for more papers by this author Megerditch Kiledjian Corresponding Author Megerditch Kiledjian Rutgers University, Department of Cell Biology and Neuroscience, 604 Allison Road, Piscataway, NJ, 08854-8082 USA Search for more papers by this author Zuoren Wang Zuoren Wang Rutgers University, Department of Cell Biology and Neuroscience, 604 Allison Road, Piscataway, NJ, 08854-8082 USA Search for more papers by this author Megerditch Kiledjian Corresponding Author Megerditch Kiledjian Rutgers University, Department of Cell Biology and Neuroscience, 604 Allison Road, Piscataway, NJ, 08854-8082 USA Search for more papers by this author Author Information Zuoren Wang1 and Megerditch Kiledjian 1 1Rutgers University, Department of Cell Biology and Neuroscience, 604 Allison Road, Piscataway, NJ, 08854-8082 USA *Corresponding author. E-mail: [email protected] The EMBO Journal (2000)19:295-305https://doi.org/10.1093/emboj/19.2.295 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Stability of the human α-globin mRNA is conferred by a ribonucleoprotein complex termed the α-complex, which acts by impeding deadenylation. Using our recently devised in vitro decay assay, we demonstrate that the α-complex also functions by protecting the 3′-untranslated region (3′-UTR) from an erythroid-enriched, sequence-specific endoribonuclease activity. The cleavage site was mapped to a region protected by the α-complex and is regulated by the presence of the α-complex. Similar endoribonuclease cleavage products were also detected in erythroid cells expressing an exogenous α-globin gene. Nucleotide substitution of the target sequence renders the RNA refractory to the endoribonuclease activity. Insertion of the target sequence onto a heterologous RNA confers sequence-specific cleavage on the chimeric RNA, demonstrating the sequence specificity of this activity. We conclude that the α-complex stabilizes the α-globin mRNA in erythroid cells by a multifaceted approach, one aspect of which is to protect the 3′-UTR from specific endoribonuclease cleavage. Introduction The steady-state accumulation of cytoplasmic mRNA is the consequence of the rates of both mRNA synthesis and degradation. Following transcription, the mRNA undergoes various processing events and is transported into the cytoplasm where it is utilized as a substrate for translation. An inherent property of each mRNA is the rate of turnover throughout the individual steps (Jacobson and Peltz, 1996). Eukaryotic mRNAs have a wide range of half-lives and their turnover is an important step in the regulation of eukaryotic gene expression. Short-lived proto-oncogene and cytokine mRNAs have half-lives of 5–30 min (Chen and Shyu, 1995), while the half-lives of some stable mRNAs are several days (Lodish and Small, 1976; Ross and Sullivan, 1985). The general stabilizing elements, which include the 5′ cap and 3′-polyadenylated tail, are found on almost all mRNAs and provide a basal level of stability by protecting the mRNA from exonuclease degradation. Differential stability of mRNAs is determined selectively by the interaction of RNA-binding proteins with cis-elements that commonly localize within the 3′-untranslated region (3′-UTR) (Jackson, 1993; Decker and Parker, 1995). Much of our current understanding of mRNA turnover comes from studies in yeast, where deadenylation followed by decapping and subsequent 5′–3′ exoribonuclease decay appears to be the major mechanism (Larimer et al., 1992; Decker and Parker, 1994; Muhlrad et al., 1995; Beelman et al., 1996; LaGrandeur and Parker, 1998). Another pathway is the 3′–5′ decay pathway, in which the deadenylated mRNA is digested directly in a 3′→5′ direction by a complex of exonucleases termed the exosome (Mitchell et al., 1997; Jacobs et al., 1998). Deadenylation also appears to be the predominant initial step in vertebrate mRNA decay as well, although this pathway is less clear (Shyu et al., 1991; Couttet et al., 1997). Distinct ribonuclease activities have been identified in higher eukaryotic cells (Schoenberg and Chernokalskaya, 1997). A poly(A)-specific 3′–5′ exoribonuclease that was cloned recently encodes a 74 kDa protein termed deadenylating nuclease (DAN) (Korner et al., 1998). The deadenylating activity of DAN could be the major activity involved in the initial phase of vertebrate mRNA turnover. A polysome-associated 33 kDa protein with 3′–5′ exonuclease activity has been identified and purified (Ross et al., 1987), yet the gene encoding this protein still remains elusive. Several endoribonuclease activities have also been identified. mRNAs targeted by endonuclease activity include those for: c-myc, insulin-like growth factor 2 (IGF-II), transferrin receptor (TfR), ApoII, Xlhbox 2B, 9E3 and albumin (Ross, 1995). Among the vertebrate endonucleases characterized, only one, a 120 kDa protein that cleaves the Xlhbox 2B mRNA, has been demonstrated to contain a sequence-specific cleavage activity (Brown et al., 1993), yet the gene remains to be identified. Recently, the gene encoding the endonuclease that cleaves Xenopus albumin mRNA was cloned. This protein, termed PMR-1, is a member of the peroxidase gene family but does not contain peroxidase activity (Chernokalskaya et al., 1998). The stability of α-globin mRNA is conferred by a cytosine-rich element (CRE) in the 3′-UTR that forms an mRNP complex (α-complex) (Wang et al., 1995; Weiss and Liebhaber, 1995). Depending on the in vitro binding conditions, the α-complex consists of either a multiprotein complex that includes the poly(C)-binding αCP1 and αCP2 proteins (Wang et al., 1995; Kiledjian et al., 1997) or the αCP1 and αCP2 proteins alone (Chkheidze et al., 1999). However, it is still unclear whether the αCP proteins alone or the multiprotein complex constitute the functional unit involved in stabilizing mRNA. In addition to a role in stabilizing α-globin mRNA, the αCP proteins have been implicated in the stability of other mRNAs including those for collagen α1(I) and tyrosine hydroxylase (Stefanovic et al., 1995; Holcik and Liebhaber, 1997; Paulding and Czyzyk-Krzeska, 1999). The αCPs (also referred to as PCBP or hnRNP E) have also been implicated in the translational regulation of 15-lipoxygenase, poliovirus, hepatitis A virus and human papillomavirus mRNAs (Blyn et al., 1997; Gamarnik and Andino, 1997; Ostareck et al., 1997; Collier et al., 1998; Graff et al., 1998). They have recently been demonstrated specifically to bind a number of mRNAs that have cytosine-rich regions including the TAPA-1 and cox II mRNAs, although the functional significance of this binding is still unknown (Trifillis et al., 1999). In vitro mRNA decay systems have greatly increased our ability to address the mechanism of vertebrate mRNA turnover (Brewer and Ross, 1990; Ross, 1993; Brewer, 1998; Ford et al., 1999; Wang et al., 1999). Reconstituted systems overcome limitations confronted in vivo that include difficulty in the detection of decay intermediates and the lack of genetic manipulations that are possible in yeast mRNA turnover analysis. We have recently devised an in vitro mRNA decay assay using the stable α-globin mRNA with post-polysomal S130 extract. Using this assay system, we demonstrated that one mechanism by which the α-complex stabilizes mRNA is by an interaction with the poly(A)-binding protein (PABP) to slow the rate of deadenylation (Wang et al., 1999). We now report that the α-complex also contributes to the stabilization of the α-globin mRNA by binding to and protecting the 3′-UTR from cleavage by a sequence-specific, erythroid-enriched endoribonuclease activity. Results Identification of an endonuclease activity in the α-globin 3′-UTR Our previous work using an in vitro decay assay (IVDA) had demonstrated that the α-complex contributes to the stabilization of the α-globin mRNA by an interaction with PABP to slow down deadenylation (Wang et al., 1999). Throughout these studies, we had occasionally detected decay intermediates that corresponded to products within the 3′-UTR. To determine the nature of these intermediates, we undertook an in vitro analysis with unadenylated RNA probe and murine erythroleukemia (MEL) cytosolic S130 extract. MEL cells maintain the α-complex-mediated stabilization of the human α-globin mRNA in vivo (Weiss and Liebhaber, 1995) as well as in vitro (data not shown). Our initial studies where decay intermediates were observed occasionally were carried out at 37°C. We reasoned that potential decay intermediates might be less stable and difficult to detect at this temperature. As shown in Figure 1, a reaction carried out at 25°C using a uniformly labeled, unadenylated α-globin wild-type 3′-UTR (αwt) probe enabled the detection of two prominent intermediate bands (Int-I and Int-II) within 5 min of incubation (lane 2). Int-II appears to be less stable and disappears quickly, while Int-1 persists for longer. Interestingly, addition of oligo(dC), which competes for and removes the α-complex (Wang et al., 1999), accentuates the appearance and persistence of both intermediates (Figure 1, compare lanes 2–5 with lanes 6–9). Therefore, the removal of the α-complex enhances the generation of the intermediates, suggesting that the α-complex might be involved in protecting the RNA from ribonuclease digestion. Interestingly, the sum of the two prominent decay intermediate sizes equals the size of the full-length 3′-UTR input RNA. This is an indication that the intermediates are the product of an endoribonuclease activity and not exoribonuclease pause sites. Figure 1.Two major decay intermediates are detected in the α-globin 3′-UTR. IVDA reactions were carried out with uniformly labeled, capped αwt 3′-UTR in MEL S130 extract at 25°C. The input RNA is shown in lane 1. Lanes 2–4 and 5–7 show the reactions in the absence or presence of oligo(dC), respectively, for the times indicated. Reactions were stopped with ULB buffer, and the resulting RNAs were isolated, resolved on an 8% polyacrylamide–7 M urea gel and visualized by autoradiography. The two major decay intermediates are denoted on the left as intermediate I (Int-I) and intermediate II (Int-II). The DNA size markers are indicated on the right of the figure in nucleotides. The horizontal line below the gel represents the RNA probe used, and the closed circle denotes the 5′ cap. The asterisks represent 32P labeling, and its presence throughout the RNA indicates that it is uniformly labeled. Download figure Download PowerPoint To determine directly whether the intermediates detected in Figure 1 are the product of an endoribonuclease activity, IVDAs were carried out with αwt RNA either uniformly labeled, 5′-end-labeled or 3′-end-labeled with 32P. Oligo(dC) was included in these reactions to detect the intermediates more readily. The RNA products were resolved on a sequencing gel to distinguish single nucleotide differences. As shown in the left panel of Figure 2, the two decay intermediates (Int-I and Int-II) are detected using a uniformly labeled αwt RNA at the initial 5 min time point, and these intermediates persist throughout the duration of the 60 min experiment (Figure 2, lanes 2–7). With increasing time, the decay intermediates accumulate and are chased into smaller products. When an αwt RNA labeled at the 5′ cap was used, only Int-I was detected (Figure 2, lanes 10–15). The use of 3′-end-labeled αwt RNA results in the detection of only Int-II (Figure 2, lanes 18–23). We therefore conclude that Int-I and II are the products of an endoribonuclease cleavage where Int-I corresponds to the 5′ fragment and Int-II to the 3′ fragment. These data demonstrate that a specific endoribonuclease cleaves the αwt RNA. Figure 2.An endoribonuclease activity targets the α-globin 3′-UTR in vitro. IVDAs were carried out in MEL S130 extract in the presence of 20 pmol of oligo(dC) at 25°C using uniformly labeled (lanes 1–7), 5′-end-labeled (lanes 9–15) or 3′-end-labeled (lanes 17–23) αwt probe. The input RNAs are shown in lanes 1, 9 and 17. Reaction times ranged from 5 to 60 min in each panel. An alkaline RNA ladder is shown in lanes 8, 16 and 24. The intermediates are indicated on the left of each panel. The labeling of the probes is indicated schematically by the asterisks under each panel. Uniformly labeled probe is as described in the legend to Figure 1. An asterisk at the left or right end of the horizontal line denotes that the probe is 5′ or 3′ end labeled, respectively. Download figure Download PowerPoint Following cleavage by the endoribonuclease, the two intermediates appear to be degraded by a 3′–5′ exoribonuclease activity. The smaller decay fragments for Int-I are identical for both uniformly labeled and 5′-end-labeled RNA (Figure 2, compare lanes 2–7 with lanes 10–15). This demonstrated that the 5′ end is intact and the decay is occurring from the 3′ end most likely by a 3′–5′ exoribonuclease. This premise is substantiated further by the decay pattern of the 3′-end-labeled Int-II. Unlike the uniformly labeled Int-II in lanes 2–7, which generates smaller fragments with increasing time, only the initial cleavage product is detected with the 3′-end-labeled Int-II (lanes 18–23). This further underscores a 3′–5′ exoribonuclease being involved in clearing the endoribonuclease cleavage products. It should also be noted that the Int-II fragment is not degraded efficiently in the 5′→3′ direction even though it does not contain a 5′ cap. These data demonstrate that both endonuclease cleavage products are cleared predominantly by a 3′–5′ exoribonuclease activity in this assay system. The endoribonuclease cleavage site was mapped using a 5′-end-labeled probe that would only detect the Int-I fragment (Figure 3A). An alkaline RNA ladder was included to identify each nucleotide, and a partial digestion of the 5′-end-labeled αwt probe with RNases U2 and T1, which cleave after adenosine and guanine residues, respectively, was also included to enable sequence identification. In agreement with the data presented in Figure 1, incubation of the 3′-UTR with S130 extract results in the accumulation of Int-I, which becomes more intense with the addition of oligo(dC) (Figure 3A, compare lanes 2 and 3 with lanes 4 and 5). These data also indicate that the cleavage product was not altered by the addition of oligo(dC). Interestingly, addition of EDTA to the reaction did not significantly affect the activity of the endoribonuclease but minimized formation of the subsequent exoribonuclease products (Figure 3A, lanes 6 and 7), which demonstrates that the endoribonuclease is less sensitive to EDTA relative to the exoribonuclease activity. A comparison of the major initial cleavage product with the alkaline RNA ladder reveals the prominent cleavage site to be 63 nucleotides downstream of the stop codon (Figure 3C). The cleavage site on the Int-II 3′ fragment was mapped using a 3′-end-labeled RNA probe. As shown in Figure 3B, the corresponding 47 nucleotide 3′ fragment is detected (lanes 2 and 3). Consistent with a role for the α-complex in protecting the target sequence, the site is contained within the region previously mapped by Holick and Liebhaber (1997) as a region protected by the α-complex (Figure 3C). Figure 3.The endoribonucleolytic cleavage site is within a region protected by the α-complex. (A) An IVDA of 5′-end-labeled RNA in MEL S130 extract is shown. Oligo(dC) was added in lanes 4 and 5, and both oligo(dC) and EDTA were added in lanes 6 and 7. The alkaline RNA, RNase U2 and RNase T1 ladders are shown in lanes 8, 9 and 10, respectively, and the corresponding RNA sizes are denoted on the right. The 5′ intermediate (Int-I) is indicated on the left. The 5′-end-labeled RNA schematic is shown at the bottom. (B) In vitro decay of 3′-end-labeled RNA in MEL S130 extract. The 3′ intermediate (Int-II) is indicated on the left and labeling is as described above. (C) The α-globin 3′-UTR sequence. The endoribonuclease cleavage site at nucleotide 63 is represented by the arrow. The shaded box represents the region protected by the α-complex as reported by Holcik and Liebhaber (1997). Download figure Download PowerPoint An endoribonuclease activity is involved in the turnover of α-globin mRNA Having identified the in vitro endoribonuclease cleavage site, we next determined whether these products could also be detected in vivo, which would indicate that the endoribonuclease can target α-globin mRNA in cells. If the in vitro pathway of α-globin mRNA turnover is representative of the in vivo pathway, fragments of 63 and 47 nucleotides, as illustrated at the bottom of Figure 4, should be produced when using an RNase protection assay. MEL cells were transfected with a plasmid expressing the human α2-globin gene and stably transformed cells (MEL-α2) were isolated. Expression of the α-globin gene in the MEL-α2 cells was shown by the presence of the protected full-length 3′-UTR in lanes 3 and 4 of Figure 4 but not in untransfected MEL cells (lane 2). In addition to the full-length intact 3′-UTR, two prominent bands corresponding to the expected sizes were also detected in MEL cells expressing the α-globin transgene (Figure 4, lanes 3 and 4). No bands were detected when RNA from untransfected MEL cells was used (lane 2). Consistent with the in vitro results, the intermediates were more stable when the cells were grown at 25°C compared with 37°C (Figure 4, compare lanes 3 and 4; see Materials and methods). The identity of the isolated Int-I and II fragments was confirmed by sequencing the reverse-transcribed and PCR-amplified RNA isolated from the gel (data not shown). These data demonstrate that the endoribonuclease activity detected in vitro is also involved in the natural turnover of α-globin mRNA in erythroid cells. Figure 4.An endoribonuclease cleaves α-globin mRNA in cells. Total RNA from MEL cells (lane 2), MEL-α2 cells grown at 37°C (lane 3) or at 25°C (lane 4) was analyzed by an RNase protection assay. A schematic diagram of the riboprobe and expected intermediate bands is shown at the bottom. The probe is antisense to the 3′-UTR (filled region) and extends 60 nucleotides downstream of the poly(A) addition site (unfilled region). The resulting RNase-resistant RNAs corresponding to the full-length 3′-UTR (110 nucleotides), Int-I (63 nucleotides) and Int-II (47 nucleotides) are indicated on the right. Download figure Download PowerPoint Sequence specificity of the endoribonuclease The sequence requirement at the cleavage site was tested by mutational analysis. Three nucleotides on either side of the cleavage site were substituted with alternating guanine and adenine dinucleotides (αmt-GA). The αmt-GA substitution prevents formation of the α-complex on the 3′-UTR (data not shown) and renders the RNA refractory to the endoribonuclease activity (Figure 5, lanes 5–8). Under the same assay conditions, the wild-type RNA is an efficient substrate (Figure 5, lanes 1–4) for this activity. Figure 5.Sequences at the endoribonuclease site are necessary. IVDA reactions were carried out with αwt RNA and αmt-GA RNA in which the six nucleotides around the endoribonucleolytic cleavage site were substituted with GAGAGA. αwt (lanes 1–4) and αmt-GA RNA (lanes 5–8) were incubated with MEL S130 extract containing oligo(dC) and EDTA for the times indicated. The arrow within the RNA schematic represents the site of cleavage and the ‘X’ depicts a block of the cleavage activity. The filled box denotes the six nucleotide substitution mutation. The intermediates and DNA size markers are as described in the legend to Figure 1. Download figure Download PowerPoint To determine whether the endoribonuclease cleavage could be conferred on a heterologous RNA, a segment of the α-globin 3′-UTR (nucleotides 37–81) that includes the endoribonuclease cleavage site was inserted into a random sequence (pGEM4 polylinker). The ability of the endoribonuclease to recognize and cleave the chimeric RNA was determined. As shown in Figure 6A, a 45 nucleotide region of the α-globin 3′-UTR was recognized and cleaved efficiently by the endoribonuclease activity (lane 2). The two resulting prominent intermediate products (Int-A and Int-B) correspond to the expected 73 and 51 nucleotide fragments. Additional background bands larger than Int-A and one smaller than Int-B are also detected. These appear to be unrelated to the α-globin sequences since comparable bands are also detected in the pGEM4 polylinker alone (Figure 6A, lane 4). Further confirmation that the intermediates are endoribonuclease products is presented in Figure 6B where 5′-end-labeled RNA was used in the assays and only the 5′ fragment (Int-A) was detected (lanes 2 and 3). The cleavage site was mapped in the chimeric RNA to the same site as that in the wild-type α-globin 3′-UTR shown in Figure 3A (data not shown). These data demonstrate that the 45 nucleotide fragment that we will refer to as the endoribonuclease recognition sequence (ERS) is an autonomous element that can be recognized and cleaved by a sequence-specific endoribonuclease activity. Figure 6.The ERS can function as an autonomous element on a heterologous RNA. (A) IVDAs were carried out in MEL S130 extract containing EDTA with uniformly labeled pGEM4 polylinker RNA (lanes 3 and 4) or uniformly labeled pGEM4 polylinker containing the 45 nucleotide ERS (lanes 1 and 2). The two endoribonuclease decay intermediates are indicated as Int-A and Int-B. A schematic of the RNA is shown at the bottom as described in the legend to Figure 1, with the open box denoting the ERS. (B) An IVDA of 5′-end-labeled pGEM4-ERS chimeric RNA (shown on the bottom) with MEL S130 extract and EDTA is shown in lanes 2 and 3. The alkaline RNA, RNase U2 and RNase T1 ladders are shown in lanes 4, 5 and 6, respectively, and the corresponding RNA size marker on the right. The 5′ endoribonuclease cleavage product is indicated as Int-A on the left. Download figure Download PowerPoint The endoribonuclease activity is ATP independent To determine whether the endoribonuclease activity requires an energy source, the decay reactions were carried out under conditions lacking ATP. As shown in Figure 7A, ATP was not required for the endoribonuclease activity since decay intermediates were detected in the absence of ATP (compare lanes 2 and 3). To ensure that the activity was not a result of residual ATP present in the extract, apyrase was also added to remove endogenous ATP (Frassetto et al., 1993). Removal of endogenous ATP also had a minimal effect on the endoribonuclease activity (Figure 7A, lane 4), although a 93 nucleotide band was detected periodically when apyrase was included. The nature and significance of this band is unknown. Furthermore, the endoribonuclease activity is present mostly in the soluble S130 extract (Figure 7B, lanes 2–4) and only residual amounts are detected in the polysomal fraction (lanes 5–7) or the ribosomal high salt wash (RSW) fraction (lanes 8–10). Therefore, the endoribonuclease activity is ATP independent and localized predominantly in the soluble cytosolic fraction, as determined by the in vitro decay conditions employed. Figure 7.The endoribonuclease activity is ATP independent and not polysome associated. (A) Uniformly labeled αwt probe (shown schematically at the bottom) was incubated with MEL S130 extract for 15 min in the presence of EDTA. ATP was included in lane 2 and excluded in lanes 3 and 4. Apyrase was also included in lane 4 to sequester endogenous ATP in the extract. The intermediates are indicated on the left. (B) IVDAs with 5′-end-labeled αwt RNA were carried out with MEL S130 extract (lanes 2–4), MEL polysomal fraction (lanes 5–7) or MEL RSW fraction (lanes 8–10) for the times indicated. The 5′ intermediate is denoted on the left, and the DNA size markers are in nucleotides. Download figure Download PowerPoint The endoribonuclease activity is enriched in erythroid cells To address the tissue specificity of the endoribonuclease activity, the IVDAs were carried out with extracts from non-erythroid cells. Extract from MEL cells (Figure 8, lanes 2 and 3) was compared with extracts from mouse hybridoma cells (SP2/0; lanes 4 and 5), macrophage cells (Raw246.7; lanes 6 and 7) and fibroblast cells (NIH 3T3; lanes 8 and 9). Under identical assay conditions, the activity of the erythroid cell extract was dramatically higher than that of the non-erythroid cell extracts. Similar results were obtained from human K562 erythroid cell extract (Figure 8B, lane 2) and non-erythroid cell extracts from B cells (Ramos; lanes 4 and 5), T cells (Jurkat; lanes 6 and 7) and fibroblasts (HeLa; lanes 8 and 9). The cleavage products are the result of a protein activity as indicated by the sensitivity of this activity to proteinase K treatment (compare lanes 2 and 3 in Figure 8A and B). Although the activity is enriched in erythroid cell extract, it is not exclusively erythroid specific since the appropriate size intermediate products are detected in the non-erythroid cells upon overexposure of the gel (data not shown). We will refer to this activity as the erythroid-enriched endoribonuclease, ErEN. These data are consistent with ErEN being involved in the regulation of α-globin mRNA turnover, since expression of α-globin is erythroid specific. Figure 8.The endoribonuclease activity is enriched in erythroid cells. (A) An IVDA was carried out with mouse erythroid S130 extract (lanes 2–3) and non-erythroid S130 extract (lanes 4–9) with uniformly labeled αwt probe in the absence or presence of proteinase K as indicated. Reactions were carried out for 15 min in the presence of oligo(dC). Labeling is as described in the legend to Figure 1. (B) IVDAs were carried out with human erythroid S130 extract (lanes 2–3) and non-erythroid S130 extract (lanes 4–9) as described above. Download figure Download PowerPoint We determined whether the coordinately expressed β-globin mRNA is also regulated by ErEN. The 3′-UTR of β-globin contains the major stability determinant of this RNA (Russell and Liebhaber, 1996). Use of the β-globin 3′-UTR in an IVDA with MEL extract revealed several low abundance intermediate bands (Figure 9, lane 5). The same intermediates are also detected with HeLa S130 extract (Figure 9, lane 6). These data demonstrate that the intermediates are not the products of ErEN, otherwise extract from the erythroid and non-erythroid cells would yield distinct products as is observed with the α-globin 3′-UTR (Figure 9, lanes 2 and 3). Therefore, ErEN can target α-globin mRNA specifically but not β-globin mRNA. Figure 9.The endoribonuclease activity is α-globin mRNA specific. IVDA reactions using uniformly labeled α-globin (lanes 1–3) or β-globin (lanes 4–6) 3′-UTRs were carried out in MEL S130 extract (lanes 2 and 5) or HeLa S130 extract (lanes 3 and 6) in the presence of oligo(dC) and EDTA for 15 min. Labeling is as described in the legend to Figure 1. Download figure Download PowerPoint The murine α-globin mRNA is cleaved by an endoribonuclease Comparison of the human α-globin (hα) ERS sequence with the corresponding region in the murine α-globin (mα) reveals an overall 60% identity, with the highest degree of conservation at the cleavage site. To test whether endoribonuclease cleavage occurs within the α-globin mRNA of other species, we determined whether the mα-3′-UTR is also targeted by an endoribonuclease. An IVDA using the 96 nucleotide mα-3′-UTR probe was carried out with MEL S130 extract. The input mα-3′-UTR probe should be cleaved in half to produce two 48 nucleotide intermediates if the position of cleavage is coincident with the hα-3′-UTR. As shown in Figure 10A, use of the mα-3′-UTR in this assay produces several bands within this size range (compare lanes 1 and 2). Wang and Liebhaber (1996) have demonstrated that the mα-3′-UTR forms an mRNA stability complex. Surprisingly, this complex is distinct from the hα-complex and contains a prominent poly(CU)-binding activity rather than a poly(C)-binding activity (Wang and Liebhaber, 1996). As shown in lane 4, addition of oligo(dCT), which competes for the mα-complex, accentuated the prevalence of the putative 48 nucleotide endoribonuclease cleavage products, while addition of a control oligonucleotide had no effect (Figure 10A, compare lanes 3 and 4). Furthermore, this activity was enriched in erythroid cells relative to non-erythroid HeLa cells even in the presence of oligo(dCT) competitor (Figure 10A, compare lanes 4 and 5). As a further confirmation that the mα-3′-UTR is targeted by an endoribonuclease activity, a second probe that contains an additional 48 nucleotides of polylinker sequence added to the 3′ terminus of the RNA was used. Cleavage of this RNA would produce the 48 nucleotide 5′ fragment as above and a 96 nucleotide 3′ fragment. As shown in Figure 10B, both the 5′ and 3′ products were detected when uniformly labeled RNA was incubated with MEL S130 extract (lane 2). Only the 5′ fragment was detected with the 5′-end-labeled RNA (Figure 10B, lane 4), and only the 3′ fragment was detected with the 3′-end-labeled RNA (lane 6). These data collectively suggest that an ErEN activity also cleaves the mα-3′-UTR in a r" @default.
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• W2003334953 date "2000-01-17" @default.
• W2003334953 modified "2023-10-17" @default.
• W2003334953 title "Identification of an erythroid-enriched endoribonuclease activity involved in specific mRNA cleavage" @default.
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