FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003382035> ?p ?o ?g. }

• W2003382035 endingPage "6" @default.
• W2003382035 startingPage "1" @default.
• W2003382035 abstract "Interference with the export of cell surface receptors can be performed through co-expression of specific affinity molecules designed for entrapment in the endoplasmic reticulum during the export process. We describe the investigation of a small (6 kDa) non-immunoglobulin-based HER2 receptor binding affibody molecule (ZHER2:00477), for use in affinity mediated entrapment of the HER2 receptor in the ER. Constructs encoding ZHER2:00477 or a control affibody protein, with or without ER-retention peptide extensions (KDEL), were expressed in the HER2 over-expressing cell line SKOV-3. Intracellular expression of the full-length affibody constructs could be confirmed by probing cell extracts by Western blotting. Confocal immunofluorescence microscopy experiments showed extensive co-localization of the HER2 receptor and ZHER2:00477-KDEL in the ER, whereas the use of a KDEL-extended control affibody molecule resulted in distinct and separate signals from cell surface-localized HER2 receptor and ER-localized affibody protein. This indicated a capability of the ZHER2:00477-KDEL fusion protein to functionally interfere with the export process of HER2 receptor in a specific manner. Using flow cytometry and cell proliferation analyses, it could be shown that expression of the ZHER2:00477-KDEL fusion construct in the SKOV-3 cell line resulted both in a marked reduction in cell surface level of HER2 receptors and that the cell population doubling time was significantly increased. Expression of the ZHER2:00477-KDEL fusion protein in additional cell lines of different origin and with different expression levels of endogenous HER2 receptor compared to SKOV-3, also resulted in depletion of the cell surface levels of HER2 receptor. This indicated upon a general ability of the ZHER2:00477-KDEL fusion protein to functionally interfere with the export process of HER2." @default.
• W2003382035 created "2016-06-24" @default.
• W2003382035 creator A5037092264 @default.
• W2003382035 creator A5052101351 @default.
• W2003382035 creator A5060045564 @default.
• W2003382035 creator A5074744219 @default.
• W2003382035 creator A5080139001 @default.
• W2003382035 date "2008-09-01" @default.
• W2003382035 modified "2023-09-30" @default.
• W2003382035 title "Affinity-based entrapment of the HER2 receptor in the endoplasmic reticulum using an affibody molecule" @default.
• W2003382035 cites W1490624051 @default.
• W2003382035 cites W1516684622 @default.
• W2003382035 cites W1567848155 @default.
• W2003382035 cites W1963903021 @default.
• W2003382035 cites W1966720839 @default.
• W2003382035 cites W1975348357 @default.
• W2003382035 cites W1985312565 @default.
• W2003382035 cites W2008024194 @default.
• W2003382035 cites W2012536704 @default.
• W2003382035 cites W2026333877 @default.
• W2003382035 cites W2029788603 @default.
• W2003382035 cites W2035758624 @default.
• W2003382035 cites W2040142253 @default.
• W2003382035 cites W2045586192 @default.
• W2003382035 cites W2046276878 @default.
• W2003382035 cites W2057520802 @default.
• W2003382035 cites W2064974685 @default.
• W2003382035 cites W2095156669 @default.
• W2003382035 cites W2099330397 @default.
• W2003382035 cites W2101803677 @default.
• W2003382035 cites W2103719918 @default.
• W2003382035 cites W2108796583 @default.
• W2003382035 cites W2116286338 @default.
• W2003382035 cites W2116361590 @default.
• W2003382035 cites W2128130662 @default.
• W2003382035 cites W2129475622 @default.
• W2003382035 cites W2134450909 @default.
• W2003382035 cites W2141393790 @default.
• W2003382035 cites W2145835877 @default.
• W2003382035 cites W2171223865 @default.
• W2003382035 cites W4237567990 @default.
• W2003382035 doi "https://doi.org/10.1016/j.jim.2008.06.005" @default.
• W2003382035 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18671978" @default.
• W2003382035 hasPublicationYear "2008" @default.
• W2003382035 type Work @default.
• W2003382035 sameAs 2003382035 @default.
• W2003382035 citedByCount "8" @default.
• W2003382035 countsByYear W20033820352012 @default.
• W2003382035 countsByYear W20033820352020 @default.
• W2003382035 crossrefType "journal-article" @default.
• W2003382035 hasAuthorship W2003382035A5037092264 @default.
• W2003382035 hasAuthorship W2003382035A5052101351 @default.
• W2003382035 hasAuthorship W2003382035A5060045564 @default.
• W2003382035 hasAuthorship W2003382035A5074744219 @default.
• W2003382035 hasAuthorship W2003382035A5080139001 @default.
• W2003382035 hasConcept C104317684 @default.
• W2003382035 hasConcept C119062480 @default.
• W2003382035 hasConcept C123894998 @default.
• W2003382035 hasConcept C143065580 @default.
• W2003382035 hasConcept C153911025 @default.
• W2003382035 hasConcept C158617107 @default.
• W2003382035 hasConcept C170493617 @default.
• W2003382035 hasConcept C185592680 @default.
• W2003382035 hasConcept C2778043179 @default.
• W2003382035 hasConcept C2779377555 @default.
• W2003382035 hasConcept C40767141 @default.
• W2003382035 hasConcept C55493867 @default.
• W2003382035 hasConcept C86803240 @default.
• W2003382035 hasConcept C95444343 @default.
• W2003382035 hasConceptScore W2003382035C104317684 @default.
• W2003382035 hasConceptScore W2003382035C119062480 @default.
• W2003382035 hasConceptScore W2003382035C123894998 @default.
• W2003382035 hasConceptScore W2003382035C143065580 @default.
• W2003382035 hasConceptScore W2003382035C153911025 @default.
• W2003382035 hasConceptScore W2003382035C158617107 @default.
• W2003382035 hasConceptScore W2003382035C170493617 @default.
• W2003382035 hasConceptScore W2003382035C185592680 @default.
• W2003382035 hasConceptScore W2003382035C2778043179 @default.
• W2003382035 hasConceptScore W2003382035C2779377555 @default.
• W2003382035 hasConceptScore W2003382035C40767141 @default.
• W2003382035 hasConceptScore W2003382035C55493867 @default.
• W2003382035 hasConceptScore W2003382035C86803240 @default.
• W2003382035 hasConceptScore W2003382035C95444343 @default.
• W2003382035 hasIssue "1-2" @default.
• W2003382035 hasLocation W20033820351 @default.
• W2003382035 hasLocation W20033820352 @default.
• W2003382035 hasOpenAccess W2003382035 @default.
• W2003382035 hasPrimaryLocation W20033820351 @default.
• W2003382035 hasRelatedWork W1986883096 @default.
• W2003382035 hasRelatedWork W2020699245 @default.
• W2003382035 hasRelatedWork W2030463413 @default.
• W2003382035 hasRelatedWork W2039787380 @default.
• W2003382035 hasRelatedWork W2063014503 @default.
• W2003382035 hasRelatedWork W2145835877 @default.
• W2003382035 hasRelatedWork W2157001035 @default.
• W2003382035 hasRelatedWork W3174109921 @default.
• W2003382035 hasRelatedWork W3210076298 @default.
• W2003382035 hasRelatedWork W4248142390 @default.

• next