FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003402857> ?p ?o ?g. }

• W2003402857 endingPage "1462" @default.
• W2003402857 startingPage "1451" @default.
• W2003402857 abstract "Abstract Objective To examine the roles of STATs 1 and 3 in CCL2 production in human osteoblastic cells and their influences on arthritis development. Methods The expression of CCL2 in primary human osteoblasts and U2OS human osteoblastic cells was examined by Northern blotting and enzyme‐linked immunosorbent assay. The roles of STAT‐1/3 and c‐Fos were assessed using short hairpin RNAs (shRNA) to silence their functions. Serine phosphorylation of STATs was assessed by Western blotting. Promoter activities of c‐Fos and CCL2 were assessed by chloramphenicol acetyltransferase and luciferase assays, respectively. Interactions of STAT‐1, STAT‐3, and c‐Fos with DNA were evaluated by electrophoretic mobility shift assay (EMSA) and immunoprecipitation. The effect of the JAK inhibitor AG‐490 on collagen‐induced arthritis (CIA) in rats was examined using immunohistochemistry. Results Oncostatin M (OSM) stimulated CCL2 expression in primary human osteoblasts and U2OS cells. In U2OS cells, STAT‐1 and STAT‐3 were involved in OSM‐stimulated CCL2 expression, and both the phosphatidylinositol 3‐kinase/Akt and MEK/ERK pathways were implicated in the activation of these STATs. STAT‐1 and STAT‐3 modulated the expression of c‐Fos and directly transactivated the CCL2 promoter. Moreover, EMSA showed formation of a DNA–protein complex containing STAT‐1, STAT‐3, and interestingly, c‐Fos. Immunoprecipitation confirmed the binding between c‐Fos and STAT‐1/3. Reporter assay revealed synergistic attenuation of CCL2 promoter activity by shRNA targeting of STAT‐1, STAT‐3, and c‐Fos. AG‐490 suppressed OSM‐stimulated activation of STAT‐1/3 and synthesis of CCL2 in vitro and diminished the severity of CIA and the number of CCL2‐synthesizing osteoblasts in vivo. Conclusion These findings show that multiple levels of STAT‐1/3 signaling modulate OSM‐stimulated CCL2 expression in human osteoblastic cells. Clinically, this pathway may be related to the pathogenesis of arthritis." @default.
• W2003402857 created "2016-06-24" @default.
• W2003402857 creator A5019561937 @default.
• W2003402857 creator A5023242114 @default.
• W2003402857 creator A5033189282 @default.
• W2003402857 creator A5036665472 @default.
• W2003402857 creator A5051348233 @default.
• W2003402857 creator A5051794947 @default.
• W2003402857 creator A5082827000 @default.
• W2003402857 creator A5089889016 @default.
• W2003402857 date "2009-04-29" @default.
• W2003402857 modified "2023-10-18" @default.
• W2003402857 title "Oncostatin M-induced CCL2 transcription in osteoblastic cells is mediated by multiple levels of STAT-1 and STAT-3 signaling: An implication for the pathogenesis of arthritis" @default.
• W2003402857 cites W127609430 @default.
• W2003402857 cites W1495596401 @default.
• W2003402857 cites W1543853846 @default.
• W2003402857 cites W1885439970 @default.
• W2003402857 cites W1898100628 @default.
• W2003402857 cites W1970704050 @default.
• W2003402857 cites W1975375148 @default.
• W2003402857 cites W1985889851 @default.
• W2003402857 cites W1991838517 @default.
• W2003402857 cites W1993034428 @default.
• W2003402857 cites W2003862811 @default.
• W2003402857 cites W2005376689 @default.
• W2003402857 cites W2014197880 @default.
• W2003402857 cites W2014531020 @default.
• W2003402857 cites W2022633707 @default.
• W2003402857 cites W2029463219 @default.
• W2003402857 cites W2030865745 @default.
• W2003402857 cites W2041766211 @default.
• W2003402857 cites W2043188148 @default.
• W2003402857 cites W2049770523 @default.
• W2003402857 cites W2052990948 @default.
• W2003402857 cites W2074206746 @default.
• W2003402857 cites W2078562739 @default.
• W2003402857 cites W2078789063 @default.
• W2003402857 cites W2085039873 @default.
• W2003402857 cites W2094111001 @default.
• W2003402857 cites W2104979180 @default.
• W2003402857 cites W2122887431 @default.
• W2003402857 cites W2127048642 @default.
• W2003402857 cites W2129362876 @default.
• W2003402857 cites W2129585918 @default.
• W2003402857 cites W2140786721 @default.
• W2003402857 cites W2141254587 @default.
• W2003402857 cites W2152519759 @default.
• W2003402857 cites W2169068585 @default.
• W2003402857 cites W2176220887 @default.
• W2003402857 cites W4313310033 @default.
• W2003402857 cites W4313342412 @default.
• W2003402857 doi "https://doi.org/10.1002/art.24452" @default.
• W2003402857 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19404962" @default.
• W2003402857 hasPublicationYear "2009" @default.
• W2003402857 type Work @default.
• W2003402857 sameAs 2003402857 @default.
• W2003402857 citedByCount "36" @default.
• W2003402857 countsByYear W20034028572012 @default.
• W2003402857 countsByYear W20034028572013 @default.
• W2003402857 countsByYear W20034028572014 @default.
• W2003402857 countsByYear W20034028572015 @default.
• W2003402857 countsByYear W20034028572016 @default.
• W2003402857 countsByYear W20034028572017 @default.
• W2003402857 countsByYear W20034028572018 @default.
• W2003402857 countsByYear W20034028572019 @default.
• W2003402857 countsByYear W20034028572020 @default.
• W2003402857 countsByYear W20034028572021 @default.
• W2003402857 countsByYear W20034028572023 @default.
• W2003402857 crossrefType "journal-article" @default.
• W2003402857 hasAuthorship W2003402857A5019561937 @default.
• W2003402857 hasAuthorship W2003402857A5023242114 @default.
• W2003402857 hasAuthorship W2003402857A5033189282 @default.
• W2003402857 hasAuthorship W2003402857A5036665472 @default.
• W2003402857 hasAuthorship W2003402857A5051348233 @default.
• W2003402857 hasAuthorship W2003402857A5051794947 @default.
• W2003402857 hasAuthorship W2003402857A5082827000 @default.
• W2003402857 hasAuthorship W2003402857A5089889016 @default.
• W2003402857 hasBestOaLocation W20034028571 @default.
• W2003402857 hasConcept C104317684 @default.
• W2003402857 hasConcept C11988809 @default.
• W2003402857 hasConcept C145430368 @default.
• W2003402857 hasConcept C153911025 @default.
• W2003402857 hasConcept C157333092 @default.
• W2003402857 hasConcept C171958077 @default.
• W2003402857 hasConcept C173396325 @default.
• W2003402857 hasConcept C183978625 @default.
• W2003402857 hasConcept C185592680 @default.
• W2003402857 hasConcept C190232843 @default.
• W2003402857 hasConcept C190283241 @default.
• W2003402857 hasConcept C203014093 @default.
• W2003402857 hasConcept C2777003663 @default.
• W2003402857 hasConcept C2777667943 @default.
• W2003402857 hasConcept C2778277574 @default.
• W2003402857 hasConcept C2778690821 @default.
• W2003402857 hasConcept C2778923194 @default.
• W2003402857 hasConcept C42362537 @default.
• W2003402857 hasConcept C502942594 @default.
• W2003402857 hasConcept C55493867 @default.

• next