Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003441429> ?p ?o ?g. }
- W2003441429 endingPage "853" @default.
- W2003441429 startingPage "845" @default.
- W2003441429 abstract "Presence in glioblastomas of cancer cells with normal neural stem cell (NSC) properties, tumor initiating capacity, and resistance to current therapies suggests that glioblastoma stem‐like cells (GSCs) play central roles in glioblastoma development. We cultured human GSCs endowed with all features of tumor stem cells, including tumor initiation after xenograft and radio‐chemoresistance. We established proteomes from four GSC cultures and their corresponding whole tumor tissues (TTs) and from human NSCs. Two‐dimensional difference gel electrophoresis and tandem mass spectrometry revealed a twofold increase of hepatoma‐derived growth factor (HDGF) in GSCs as compared to TTs and NSCs. Western blot analysis confirmed HDGF overexpression in GSCs as well as its presence in GSC‐conditioned medium, while, in contrast, no HDGF was detected in NSC secretome. At the functional level, GSC‐conditioned medium induced migration of human cerebral endothelial cells that can be blocked by anti‐HDGF antibodies. In vivo, GSC‐conditioned medium induced neoangiogenesis, whereas HDGF‐targeting siRNAs abrogated this effect. Altogether, our results identify a novel candidate, by which GSCs can support neoangiogenesis, a high‐grade glioma hallmark. Our strategy illustrates the usefulness of comparative proteomic analysis to decipher molecular pathways, which underlie GSC properties. Disclosure of potential conflicts of interest is found at the end of this article." @default.
- W2003441429 created "2016-06-24" @default.
- W2003441429 creator A5017113646 @default.
- W2003441429 creator A5018387769 @default.
- W2003441429 creator A5022317593 @default.
- W2003441429 creator A5029335720 @default.
- W2003441429 creator A5037724430 @default.
- W2003441429 creator A5042534464 @default.
- W2003441429 creator A5043560581 @default.
- W2003441429 creator A5059755071 @default.
- W2003441429 creator A5060230145 @default.
- W2003441429 creator A5068555614 @default.
- W2003441429 creator A5077011745 @default.
- W2003441429 creator A5014967891 @default.
- W2003441429 date "2012-04-09" @default.
- W2003441429 modified "2023-10-05" @default.
- W2003441429 title "Differential Proteomic Analysis of Human Glioblastoma and Neural Stem Cells Reveals HDGF as a Novel Angiogenic Secreted Factor" @default.
- W2003441429 cites W1546669442 @default.
- W2003441429 cites W1736470562 @default.
- W2003441429 cites W1845986149 @default.
- W2003441429 cites W1892965527 @default.
- W2003441429 cites W1971992755 @default.
- W2003441429 cites W1976488065 @default.
- W2003441429 cites W1978041336 @default.
- W2003441429 cites W1979747387 @default.
- W2003441429 cites W1980139116 @default.
- W2003441429 cites W1981705266 @default.
- W2003441429 cites W1985483310 @default.
- W2003441429 cites W1987144487 @default.
- W2003441429 cites W1995054026 @default.
- W2003441429 cites W1995864540 @default.
- W2003441429 cites W1999553354 @default.
- W2003441429 cites W2002208297 @default.
- W2003441429 cites W2007888447 @default.
- W2003441429 cites W2016080154 @default.
- W2003441429 cites W2018671779 @default.
- W2003441429 cites W2021916242 @default.
- W2003441429 cites W2025972963 @default.
- W2003441429 cites W2026036433 @default.
- W2003441429 cites W2028943826 @default.
- W2003441429 cites W2029622598 @default.
- W2003441429 cites W2033469836 @default.
- W2003441429 cites W2038516559 @default.
- W2003441429 cites W2039588396 @default.
- W2003441429 cites W2040354740 @default.
- W2003441429 cites W2047394764 @default.
- W2003441429 cites W2048244201 @default.
- W2003441429 cites W2048281866 @default.
- W2003441429 cites W2050106965 @default.
- W2003441429 cites W2054235977 @default.
- W2003441429 cites W2057179701 @default.
- W2003441429 cites W2057484801 @default.
- W2003441429 cites W2059712501 @default.
- W2003441429 cites W2060551076 @default.
- W2003441429 cites W2066715837 @default.
- W2003441429 cites W2068229533 @default.
- W2003441429 cites W2068262573 @default.
- W2003441429 cites W2071547624 @default.
- W2003441429 cites W2072424849 @default.
- W2003441429 cites W2073180939 @default.
- W2003441429 cites W2073759231 @default.
- W2003441429 cites W2082210456 @default.
- W2003441429 cites W2089273979 @default.
- W2003441429 cites W2093797379 @default.
- W2003441429 cites W2096287682 @default.
- W2003441429 cites W2105279254 @default.
- W2003441429 cites W2106675906 @default.
- W2003441429 cites W2107798346 @default.
- W2003441429 cites W2111375311 @default.
- W2003441429 cites W2113942802 @default.
- W2003441429 cites W2121927401 @default.
- W2003441429 cites W2122532981 @default.
- W2003441429 cites W2131883841 @default.
- W2003441429 cites W2133117291 @default.
- W2003441429 cites W2134400415 @default.
- W2003441429 cites W2138879032 @default.
- W2003441429 cites W2141600996 @default.
- W2003441429 cites W2141796595 @default.
- W2003441429 cites W2142079389 @default.
- W2003441429 cites W2144867791 @default.
- W2003441429 cites W2144977640 @default.
- W2003441429 cites W2146369144 @default.
- W2003441429 cites W2147593703 @default.
- W2003441429 cites W2151272215 @default.
- W2003441429 cites W2152387543 @default.
- W2003441429 cites W2153931867 @default.
- W2003441429 cites W2159409883 @default.
- W2003441429 cites W2160120650 @default.
- W2003441429 cites W2160237840 @default.
- W2003441429 cites W2160382843 @default.
- W2003441429 cites W2161289668 @default.
- W2003441429 cites W2163226383 @default.
- W2003441429 cites W2165357348 @default.
- W2003441429 cites W2166184689 @default.
- W2003441429 cites W2166556978 @default.
- W2003441429 cites W2168414172 @default.
- W2003441429 cites W2906916700 @default.
- W2003441429 doi "https://doi.org/10.1002/stem.1062" @default.